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Product Info Summary
| SKU: | M00894-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-CALR Antibody (Center)
SKU/Catalog Number
M00894-1
Size
50 µl
Description
Boster Bio Anti-CALR Antibody (Center) (Catalog # M00894-1). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CALR Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00894-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2143CT58.2.1
Isotype
IgG1,κ
Immunogen
This CALR antibody is generated from a mouse immunized with a recombinant protein from human CALR.
Reactive Species
M00894-1 is reactive to CALR in Human, Mouse, Rat
Calculated molecular weight
48.1 kDa
Background of CALR
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00894-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
Validation Images & Assay Conditions
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All lanes : Anti-CALR Antibody (Center) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: HeLa whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: SH-SY5Y whole cell lysate
Lane 6: K562 whole cell lysate
Lane 7: NIH/3T3 whole cell lysate Lane 8: C6 whole cell lysate Lane 9: CHO whole cell lysate Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 55 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-CALR Antibody (Center) at dilution + Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 48 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-CALR Antibody (Center) at dilution
Lane 1:HepG2 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: SH-SY5Y whole cell lysate
Lane 6: K562 whole cell lysate
Lane 7: NIH/3T3 whole cell lysate
Lane 8: C6 whole cell lysate
Lane 9: CHO whole cell lysat
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 48 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-CALR Antibody (Center) (M00894-1)
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