Anti-Phospho-CaM Kinase II (Thr306) iucD Antibody

Product Name

Anti-Phospho-CaM Kinase II (Thr306) iucD Antibody

View all ACADM Antibodies

SKU/Catalog Number

P30406

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-CaM Kinase II (Thr306) iucD Antibody (Catalog # P30406). Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-CaM Kinase II (Thr306) iucD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30406)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr306 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P30406 is reactive to ACADM in Mouse, Rat

Applications

P30406 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

46.588kDa

Background of ACADM

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr-286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). CaMKIIα autophosphorylation at Thr-286 and Thr-305/Thr-306 has recently been shown to regulate kinase activity and modulate subcellular targeting and is critical for normal synaptic plasticity and learning and memory (Baucum et al., 2015).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa alpha-and the ~60 kDa beta-CaM Kinase II phosphorylated at Thr³⁰⁶ in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (gamma-Ptase, 800 units/1mg protein for 30 minutes).

Gene/Protein Information For ACADM (Source: Uniprot.org, NCBI)

Alternative Names

ACAD1; acyl-CoA dehydrogenase, C-4 to C-12 straight chain; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; EC 1.3.99; EC 1.3.99.3; FLJ18227; FLJ99884; MCADFLJ93013; MCADH; medium-chain specific acyl-CoA dehydrogenase, mitochondrial ACADM ACAD1, MCAD, MCADH acyl-CoA dehydrogenase medium chain medium-chain specific acyl-CoA dehydrogenase, mitochondrial|acyl-CoA dehydrogenase, C-4 to C-12 straight chain|acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain|medium-chain acyl-CoA dehydrogenase|testicular tissue protein Li 7

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