Product Info Summary
SKU: | A09186-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Carbonic Anhydrase 13/CA13 Antibody Picoband™
View all Carbonic Anhydrase XIII/CA13 Antibodies
SKU/Catalog Number
A09186-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Carbonic Anhydrase 13/CA13 Antibody Picoband™ catalog # A09186-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Carbonic Anhydrase 13/CA13 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09186-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase 13/CA13, which shares 85% and 55% amino acid (aa) sequence identity with mouse and rat Carbonic Anhydrase 13/CA13, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09186-3 is reactive to CA13 in Human, Mouse, Rat
Applications
A09186-3 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
29 kDa
Calculated molecular weight
29.443kDa
Background of Carbonic Anhydrase XIII/CA13
Carbonic anhydrase 13 is a protein that in humans is encoded by the CA13 gene. Carbonic anhydrases (CAs) are a family of zinc metalloenzymes that catalyze the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Carbonic Anhydrase 13/CA13 using anti-Carbonic Anhydrase 13/CA13 antibody (A09186-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: rat intestines tissue lysates,
Lane 7: mouse NTH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 13/CA13 antigen affinity purified polyclonal antibody (Catalog # A09186-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 13/CA13 at approximately 29KD. The expected band size for Carbonic Anhydrase 13/CA13 is at 29KD.
Click image to see more details
Figure 2. IHC analysis of Carbonic Anhydrase 13/CA13 using anti-Carbonic Anhydrase 13/CA13 antibody (A09186-3).
Carbonic Anhydrase 13/CA13 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Carbonic Anhydrase 13/CA13 Antibody (A09186-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Carbonic Anhydrase 13/CA13 using anti-Carbonic Anhydrase 13/CA13 antibody (A09186-3).
Carbonic Anhydrase 13/CA13 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Carbonic Anhydrase 13/CA13 Antibody (A09186-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Carbonic Anhydrase 13/CA13 using anti-Carbonic Anhydrase 13/CA13 antibody (A09186-3).
Carbonic Anhydrase 13/CA13 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Carbonic Anhydrase 13/CA13 Antibody (A09186-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Carbonic Anhydrase 13/CA13 using anti-Carbonic Anhydrase 13/CA13 antibody (A09186-3).
Carbonic Anhydrase 13/CA13 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Carbonic Anhydrase 13/CA13 Antibody (A09186-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of Carbonic Anhydrase 13/CA13 using anti-Carbonic Anhydrase 13/CA13 antibody (A09186-3).
Carbonic Anhydrase 13/CA13 was detected in immunocytochemical section of Hep cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Carbonic Anhydrase 13/CA13 Antibody (A09186-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. Flow Cytometry analysis of CACO-2 cells using anti-Carbonic Anhydrase 13/CA13 antibody (A09186-3).
Overlay histogram showing CACO-2 cells stained with A09186-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Carbonic Anhydrase 13/CA13 Antibody (A09186-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CA13 (Source: Uniprot.org, NCBI)
Gene Name
CA13
Full Name
Carbonic anhydrase 13
Weight
29.443kDa
Superfamily
alpha-carbonic anhydrase family
Alternative Names
CA13; carbonic anhydrase 13; Carbonic Anhydrase XIII; carbonic anhydrase XIIICarbonate dehydratase XIII; CAXIII; CA-XIII; EC 4.2.1.1; FLJ37995; MGC59868 CA13 CAXIII carbonic anhydrase 13 carbonic anhydrase 13|CA-XIII|carbonate dehydratase XIII|carbonic anhydrase XIII
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CA13, check out the CA13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CA13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Carbonic Anhydrase 13/CA13 Antibody Picoband™ (A09186-3)
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