Product Info Summary
SKU: | A01083-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband™
View all Carbonic Anhydrase IX/CA9 Antibodies
SKU/Catalog Number
A01083-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband™ catalog # A01083-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01083-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human Carbonic Anhydrase 9/CA9 recombinant protein (Position: D146-D414).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01083-3 is reactive to CA9 in Human, Mouse
Applications
A01083-3 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Background of Carbonic Anhydrase IX/CA9
Carbonic anhydrase IX (CA9/CA IX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry, 1-3 μg/1x10^6 cells, Human, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions

Click image to see more details
Figure 1. Western blot analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 9/CA9 antigen affinity purified polyclonal antibody (Catalog # A01083-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 9/CA9 at approximately 54 kDa. The expected band size for Carbonic Anhydrase 9/CA9 is at 50 kDa.

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Figure 2. IHC analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3).
Carbonic Anhydrase 9/CA9 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3).
Carbonic Anhydrase 9/CA9 was detected in a paraffin-embedded section of human renal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Click image to see more details
Figure 4. IHC analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3).
Carbonic Anhydrase 9/CA9 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 5. Flow Cytometry analysis of U87 cells using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3).
Overlay histogram showing U87 cells stained with A01083-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 6. Flow Cytometry analysis of RAW264.7 cells using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3).
Overlay histogram showing RAW264.7 cells stained with A01083-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CA9 (Source: Uniprot.Org, NCBI)
Uniprot ID
Q16790
Gene ID
768
Gene Name
CA9
Full Name
Carbonic anhydrase 9
Weight
49.698kDa
Superfamily
alpha-carbonic anhydrase family
Alternative Names
CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250 CA9 CAIX, MN carbonic anhydrase 9 carbonic anhydrase 9|CA-IX|P54/58N|RCC-associated antigen G250|RCC-associated protein G250|carbonate dehydratase IX|carbonic anhydrase IX|carbonic dehydratase|membrane antigen MN|pMW1|renal cell carcinoma-associated antigen G250
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CA9, check out the CA9 Infographic

We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CA9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband™ (A01083-3)
No publications found for A01083-3
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