NLRC4 Antibodies

AND NLRC4 ELISA kits, NLRC4 Proteins

Many biological assays use antibodies to detect NLRC4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with NLRC4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop NLRC4 antibodies...

We validate the specificity of these antibodies to NLRC4 by testing them on tissues known to express NLRC4 positively and negatively. Browse below to find the NLRC4 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for NLRC4? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the NLRC4 Western Blot Guide

NLRC4 Infographic by Boster Bio

All NLRC4 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-CARD12/NLRC4 Antibody Picoband®

2 Citations

Western blot analysis of CARD12 using anti-CARD12 antibody (PB9673)

Human, Mouse, Rat

IF, ICC, WB

$370
Cat # PB9673
100 μg/vial

Anti-Ipaf NLRC4 Antibody

Western blot analysis of Ipaf in human PBL lysate with Ipaf antibody at 0

Immunocytochemistry of Ipaf in THP-1 cells with Ipaf antibody at 10 μg/mL

Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 μg/mL

Human

ELISA, IF, ICC, WB

$481
Cat # A00525
0.1 mg

CARD12 (NLRC4) (NM_021209) Human Over-expression Lysate

Human

$628
Cat # LS058484
20 µg, 100 µg

NLRC4 Overview

Facts about NLR family CARD domain-containing protein 4.

NLRC4 3D structure. Source: alphafold

NLR family CARD domain-containing protein 4 (NLRC4)

Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and reacts by building an inflammasome complex that boosts caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).

Gene Information

Human
Gene Name:	NLRC4
Uniprot:	Q9NPP4
Entrez:	58484

Family

Belongs to:

No superfamily

Alternative Names

CARD12leucine rich repeat and CARD domaincontaining 4; caspase recruitment domain family, member 12; Caspase recruitment domain-containing protein 12; Clan protein; CLAN1ipaf; CLANCARD, LRR, and NACHT-containing protein; CLR2.1; Ice protease-activating factor; Ipaf; NLR family, CARD domain containing 4

Molecular Weight

Mass (kDA):
116.159 kDA

Genomic Context


Human

Location:	2p22.3
Sequence:	2; NC_000002.12 (32224449..32265743, complement)

Tissue Specificity

Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.

Subcellular Localizations

Cytoplasm. Cytoplasm, cytosol. Cytoplasmic filaments.

Diseases

Inflammation
Salmonella Infections
Legionnaires' Disease
Bacterial Infections
Innate Immune Response
Infective Disorder
Malignant Neoplasms
Pneumonia
Neoplasms
Pseudomonas Infections
Shigella Infections
Permissiveness, Biological Function
Disease Caused By Shigella Flexneri

Inflammatory Response
Cell Invasion
Gram-negative Bacterial Infections
Autoimmune Diseases
Peritonitis
Colitis
Cell Transformation, Neoplastic

Interacting Proteins

CASP1

Pathways

Secretion
Cell Death
Pyroptosis
Immune Response
Pathogenesis
Innate Immune Response
Virulence
Inflammatory Response
Cytokine Production
Rna Interference
Autophagy
Transport
Programmed Cell Death

Phagosome Maturation
Epithelial Cell Proliferation
Cell Proliferation
Dna Repair
Tissue Homeostasis
Gene Silencing
Phagosome Acidification

PTMs

Cleavage
Phosphorylation

Nitrosylation
Ubiquitination

Research Areas

Apoptosis
Immunology
Innate Immunity

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NLRC4

References

PMID: 11374873 by Geddes B.J., et al. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis.

PMID: 11472070 by Damiano J.S., et al. CLAN, a novel human CED-4-like gene.