Anti-CARD8 Antibody

Product Name

Anti-CARD8 Antibody

View all CARD8 Antibodies

SKU/Catalog Number

A03404-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CARD8 Antibody (Catalog # A03404-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CARD8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03404-1)

Host

Rabbit

Contents

CARD8 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8. The immunogen is located within the last 50 amino acids of CARD8.

Reactive Species

A03404-1 is reactive to CARD8 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

48.9 kDa

Background of CARD8

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-κB activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03404-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CARD8 antibody can be used for detection of CARD8 by Western blot at 1 -2 μg/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CARD8

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