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Product Info Summary
| SKU: | M03475 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Casein Kinase 2 beta Monoclonal Antibody
View all Casein Kinase 2 beta Antibodies
SKU/Catalog Number
M03475
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Casein Kinase 2 beta Monoclonal Antibody catalog # M03475. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Casein Kinase 2 beta Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03475)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEHG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Casein Kinase 2 beta Participates in Wnt signaling (By similarity) . Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03475 is reactive to CSNK2B in Human, Mouse, Rat
Applications
M03475 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
106 kDa
Calculated molecular weight
24.942kDa
Background of Casein Kinase 2 beta
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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Western blot analysis of Casein Kinase 2 beta expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CSNK2B (Source: Uniprot.org, NCBI)
Gene Name
CSNK2B
Full Name
Casein kinase II subunit beta
Weight
24.942kDa
Superfamily
casein kinase 2 subunit beta family
Alternative Names
Casein Kinase 2 beta; casein kinase 2, beta polypeptide; Casein kinase II beta subunit; casein kinase II subunit beta; CK II beta; CK2B; CK2N; CSK2B; CSNK2B; G5A; MGC138222; MGC138224; Phosvitin; Protein G5a CSNK2B CK2B, CK2N, CSK2B, Ckb1, Ckb2, G5A, POBINDS casein kinase 2 beta casein kinase II subunit beta|CK II beta|CSNK2B-LY6G5B-1072|CSNK2B-LY6G5B-1103|CSNK2B-LY6G5B-532|CSNK2B-LY6G5B-560|CSNK2B-LY6G5B-562|Casein kinase II beta subunit|alternative name: G5a, phosvitin|casein kinase 2, beta polypeptide|phosvitin|protein G5a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSNK2B, check out the CSNK2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSNK2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Casein Kinase 2 beta Monoclonal Antibody (M03475)
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No publications found for M03475
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Casein Kinase 2 beta Monoclonal Antibody
Question
We are currently using anti-Casein Kinase 2 beta Monoclonal antibody M03475 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
The anti-Casein Kinase 2 beta Monoclonal antibody (M03475) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-23
Question
Can you help my question with product M03475, anti-Casein Kinase 2 beta Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03475 anti-Casein Kinase 2 beta Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-26
Question
I was wanting to use your anti-Casein Kinase 2 beta Monoclonal antibody for WB for mouse right testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right testis identification?
M. Parker
Verified customer
Asked: 2018-09-05
Answer
As indicated on the product datasheet, M03475 anti-Casein Kinase 2 beta Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse right testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-05
Question
Is there a BSA free version of anti-Casein Kinase 2 beta Monoclonal antibody M03475 available?
L. Zhao
Verified customer
Asked: 2013-01-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Casein Kinase 2 beta Monoclonal antibody M03475 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-21
