Anti-NCKAP1 Antibody Picoband®

NCKAP1 antibody

Boster Bio Anti-NCKAP1 Antibody Picoband® catalog # A09238-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A09238-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-NCKAP1 Antibody Picoband®

View all NCKAP1 Antibodies

SKU/Catalog Number

A09238-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NCKAP1 Antibody Picoband® catalog # A09238-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NCKAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09238-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NCKAP1 recombinant protein (Position: S2-K201). Human NCKAP1 shares 98.5% and 98% amino acid (aa) sequence identity with mouse and rat NCKAP1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09238-1 is reactive to NCKAP1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

129 kDa

Calculated molecular weight

128790 MW

Background of NCKAP1

Nck-associated protein 1 is a protein that in humans is encoded by the NCKAP1 gene. By somatic cell hybrid analysis, the NCKAP1 gene was mapped to chromosome 2. NCKAP1 is a predicted type II transmembrane protein, with a C-terminal transmembrane domain. It was demonstrated that suppression of NCKAP1 expression using an antisense NCKAP1 oligonucleotide induced apoptosis of neuronal cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09238-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: rat brain tissue, mouse brain tissue, MCF-7 whole Cell, 293T whole cell

Validation Images & Assay Conditions

Gene/Protein Information For NCKAP1 (Source: Uniprot.org, NCBI)

Gene Name

NCKAP1

Full Name

Nck-associated protein 1

Weight

128790 MW

Superfamily

HEM-1/HEM-2 family

Alternative Names

Nck-associated protein 1;NAP 1;Membrane-associated protein HEM-2;p125Nap1;NCKAP1;HEM2, KIAA0587, NAP1; NCKAP1 HEM2, NAP1, NAP125, p125Nap1 NCK associated protein 1 nck-associated protein 1|membrane-associated protein HEM-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NCKAP1, check out the NCKAP1 Infographic

NCKAP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NCKAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-NCKAP1 Antibody Picoband®

Question

We are currently using anti-NCKAP1 antibody A09238-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

J. Baker

Verified customer

Asked: 2020-01-03

Answer

The anti-NCKAP1 antibody (A09238-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-03

Question

We need to test anti-NCKAP1 antibody A09238-1 on rat brain for research purposes, then I may be interested in using anti-NCKAP1 antibody A09238-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

The products we sell, including anti-NCKAP1 antibody A09238-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-31

Question

See attached the WB image, lot number and protocol we used for brain using anti-NCKAP1 antibody A09238-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-22

Question

Would anti-NCKAP1 antibody A09238-1 work for WB with brain?

B. Mitchell

Verified customer

Asked: 2018-10-05

Answer

According to the expression profile of brain, NCKAP1 is highly expressed in brain. So, it is likely that anti-NCKAP1 antibody A09238-1 will work for WB with brain.

Boster Scientific Support

Answered: 2018-10-05

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-NCKAP1 antibody A09238-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-05-11

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-11

Order DetailsPrice
A09238-1

100μg

$370
A09238-1-10ug

10μg sample (liquid)

$99
A09238-1-Biotin

100 μg Biotin conjugated

$570
A09238-1-Cy3

100 μg Cy3 conjugated

$570
A09238-1-Dylight488

100 μg Dylight488 conjugated

$570
A09238-1-Dylight550

100 μg Dylight550 conjugated

$570
A09238-1-Dylight594

100 μg Dylight594 conjugated

$570
A09238-1-FITC

100 μg FITC conjugated

$570
A09238-1-HRP

100 μg HRP conjugated

$570
A09238-1-APC

100 μg APC conjugated

$670
A09238-1-PE

100 μg PE conjugated

$670
A09238-1-iFluor647

100 μg iFluor647 conjugated

$670
A09238-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A09238-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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