Click image to see more details
Product Info Summary
| SKU: | A09238-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NCKAP1 Antibody Picoband®
SKU/Catalog Number
A09238-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NCKAP1 Antibody Picoband® catalog # A09238-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NCKAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09238-1)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NCKAP1 recombinant protein (Position: S2-K201). Human NCKAP1 shares 98.5% and 98% amino acid (aa) sequence identity with mouse and rat NCKAP1, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09238-1 is reactive to NCKAP1 in Human, Mouse, Rat
Observed Molecular Weight
129 kDa
Calculated molecular weight
128.8 kDa
Background of NCKAP1
Nck-associated protein 1 is a protein that in humans is encoded by the NCKAP1 gene. By somatic cell hybrid analysis, the NCKAP1 gene was mapped to chromosome 2. NCKAP1 is a predicted type II transmembrane protein, with a C-terminal transmembrane domain. It was demonstrated that suppression of NCKAP1 expression using an antisense NCKAP1 oligonucleotide induced apoptosis of neuronal cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09238-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue, MCF-7 whole Cell, 293T whole cell
Validation Images & Assay Conditions
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Western blot analysis of NCKAP1 using anti-NCKAP1 antibody (A09238-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: MCF-7 whole Cell lysates,
Lane 4: 293T whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCKAP1 antigen affinity purified polyclonal antibody (Catalog # A09238-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCKAP1 at approximately 129KD. The expected band size for NCKAP1 is at 129KD.
Specific Publications For Anti-NCKAP1 Antibody Picoband® (A09238-1)
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Customer Q&As
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5 Customer Q&As for Anti-NCKAP1 Antibody Picoband®
Question
We are currently using anti-NCKAP1 antibody A09238-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
J. Baker
Verified customer
Asked: 2020-01-03
Answer
The anti-NCKAP1 antibody (A09238-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-03
Question
We need to test anti-NCKAP1 antibody A09238-1 on rat brain for research purposes, then I may be interested in using anti-NCKAP1 antibody A09238-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The products we sell, including anti-NCKAP1 antibody A09238-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-31
Question
See attached the WB image, lot number and protocol we used for brain using anti-NCKAP1 antibody A09238-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-22
Question
Would anti-NCKAP1 antibody A09238-1 work for WB with brain?
B. Mitchell
Verified customer
Asked: 2018-10-05
Answer
According to the expression profile of brain, NCKAP1 is highly expressed in brain. So, it is likely that anti-NCKAP1 antibody A09238-1 will work for WB with brain.
Boster Scientific Support
Answered: 2018-10-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-NCKAP1 antibody A09238-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-11
