Product Info Summary
| SKU: | A13821 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Caskin-2 CASKIN2 Antibody
SKU/Catalog Number
A13821
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Caskin-2 CASKIN2 Antibody (Catalog # A13821). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
CASKIN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Caskin-2 CASKIN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13821)
Host
Rabbit
Contents
CASKIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CASKIN2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human CASKIN2. The immunogen is located within amino acids 380 - 430 of CASKIN2.
Cross-reactivity
CASKIN2 antibody is predicted to not cross-react with other CASKIN family members. Three isoforms of CASKIN2 are known to exist; this antibody will not detect the shortest isoform.
Reactive Species
A13821 is reactive to CASKIN2 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
126.8 kDa
Background of CASKIN2
Calcium/calmodulin-dependent serine protein kinase (CASK) is a conserved multi-domain scaffolding protein involved in brain development, synapse formation, and establishment of cell polarity. CASKINs (CASK interacting protein ) interact with CASK and is thought to play a role in CASK function, specifically by coupling CASK to distinct downstream effectors. CASKIN2 is a multidomain protein containing six N-terminal ankyrin repeats, one SH3 domain, and two sterile alpha motif domains followed by a long proline-rich sequence and a short conserved C-terminal domain. It is expressed ubiquitously with highest levels present in fetal and adult liver tissue.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13821 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CASKIN2 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.
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Immunocytochemistry of CASKIN2 in HeLa cells with CASKIN2 antibody at 2.5 μg/mL.
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Immunofluorescence of CASKIN2 in HeLa cells with CASKIN2 antibody at 20 μg/mL.
Specific Publications For Anti-Caskin-2 CASKIN2 Antibody (A13821)
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