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Product Info Summary
| SKU: | M02190-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-Caspase-10 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02190-1
BM4811 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Caspase-10 Rabbit Monoclonal Antibody catalog # M02190-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02190-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ICH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Caspase-10
Reactive Species
M02190-1 is reactive to CASP10 in Human
Observed Molecular Weight
59 kDa
Calculated molecular weight
59.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02190-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Caspase-10 expression in Jurkat cell lysate.
Specific Publications For Anti-Caspase-10 Rabbit Monoclonal Antibody (M02190-1)
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Customer Reviews
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6 Customer Q&As for Anti-Caspase-10 Rabbit Monoclonal Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen thymus using anti-Caspase-10 Rabbit Monoclonal antibody M02190-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
See below the WB image, lot number and protocol we used for spleen thymus using anti-Caspase-10 Rabbit Monoclonal antibody M02190-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-02
Question
I was wanting to use your anti-Caspase-10 Rabbit Monoclonal antibody for WB for human spleen thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human spleen thymus identification?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
You can see on the product datasheet, M02190-1 anti-Caspase-10 Rabbit Monoclonal antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-17
Question
Is this M02190-1 anti-Caspase-10 Rabbit Monoclonal antibody reactive to the isotypes of CASP10?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
The immunogen of M02190-1 anti-Caspase-10 Rabbit Monoclonal antibody is A synthesized peptide derived from human Caspase-10. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-23
Question
I see that the anti-Caspase-10 Rabbit Monoclonal antibody M02190-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-04-12
Question
I have a question about product M02190-1, anti-Caspase-10 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Miller
Verified customer
Asked: 2014-07-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02190-1 anti-Caspase-10 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-07-23
