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Product Info Summary
| SKU: | PA1302-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Caspase-3 (P10)/CASP3 Antibody Picoband®
SKU/Catalog Number
PA1302-1
BA3592 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-3 (P10)/CASP3 Antibody catalog # PA1302-1. Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-3 (P10)/CASP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1302-1)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-3(p10).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1302-1 is reactive to CASP3 in Human
Observed Molecular Weight
51 kDa
Calculated molecular weight
31.6 kDa
Background of CASP3
Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1302-1 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry , 0.5-1μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Cell, SMMC Cell
IHC: Human Intestinal Cancer Tissue, Human Lung Cancer Tissue
ICC: A549 cell
Validation Images & Assay Conditions
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Anti-Caspase-3(P10), PA1302-1, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-Caspase-3(P10), PA1302-1, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
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Anti-Caspase-3(P10), PA1302-1, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Effects of ZXC on the mRNA levels of the Bcl-2/Bax ratio (A) , caspase-3 (B) , nuclear factor (NF)-кB (C) , and p38 (D) in the prefrontal cortex of ischemia-reperfusion injury rats. The data are expressed as mean ± standard deviation (n = 3). I-30, ischemia for 30 min; I-90, ischemia for 90 min; I-90+R-30, ischemia for 90 min, then reperfusion for 30 min; I-90+R-180, ischemia for 90 min, then reperfusion for 180 min. * P < 0.05 vs. sham group; # P < 0.05 vs. model group; ## P < 0.01 vs. model group.
Index in PubMed under a CC BY license. PMID: 31611791
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Western blotting results for Sham, Model and ZXC groups (A) . Effects of ZXC on the protein expressions Bcl-2 (B) , Bax (C) , Caspase-3 (D) , NF-кB (E) , and p38 (F) in brain tissue of ischemia-reperfusion injury rats induced by MCAO. The data are expressed as mean ± standard deviation (n = 4). I-30, ischemia for 30 min; I-90, ischemia for 90 min; I-90+R-30, ischemia for 90 min, then reperfusion for 30 min. *P < 0.05 vs. sham group; # P < 0.05 vs. model group.
Index in PubMed under a CC BY license. PMID: 31611791
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ICC analysis of Caspase-3(P10) using anti-Caspase-3(P10) antibody (PA1302-1).
Caspase-3(P10) was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Caspase-3(P10) Antibody (PA1302-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of caspase-3 (P10)/CASP3 using anti-caspase-3 (P10)/CASP3 antibody (PA1302-1).
caspase-3 (P10)/CASP3 was detected in a rat spinal cord. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with peroxidase blocker. The tissue section was then incubated with 2 μg/ml anti-caspase-3 (P10)/CASP3 antibody (PA1302-1) at 1:200 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated at 1:500 for 1h at room temperature. The tissue section was developed using fluorescent microscope.
Specific Publications For Anti-Caspase-3 (P10)/CASP3 Antibody Picoband® (PA1302-1)
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Customer Reviews
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1 Reviews For Anti-Caspase-3 (P10)/CASP3 Antibody Picoband®
Excellent Caspase-3 Antibody from Boster Bio--Mostafa Gabr, Duke University Hospital, Neurosurgery, Postdoctoral Researcher
Excellent
Source: Biocompare.com
| SKU | PA1302-1 |
|---|---|
| Application | Immunohistochemistry |
| Sample | Rat spinal cord |
| Detection | Fluorescent microscope |
"I purchased this product to stain rat spinal cords exposed to injury. The injured spinal cords showed apoptotic cells obviously. Injured spinal cord sample showed high apoptosis compared to control (uninjured) spinal cord. Excellent, reliable product and easy to use."
Mostafa Gabr
Verified customer
Submitted 2019-05-21
Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
17 Customer Q&As for Anti-Caspase-3 (P10)/CASP3 Antibody Picoband®
Question
I see that the anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-22
Question
We have observed staining in human cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-Caspase-3 (P10)/CASP3 antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
From what I have seen in literature cervix carcinoma erythroleukemia does express CASP3. From what I have seen in Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-14
Question
We need to test anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 on human jejunal mucosa for research purposes, then I may be interested in using anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The products we sell, including anti-Caspase-3 (P10)/CASP3 antibody PA1302-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-06
Question
Is a blocking peptide available for product anti-Caspase-3 (P10)/CASP3 antibody (PA1302-1)?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
We do provide the blocking peptide for product anti-Caspase-3 (P10)/CASP3 antibody (PA1302-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-25
Question
I was wanting to use your anti-Caspase-3 (P10)/CASP3 antibody for ICC for human jejunal mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human jejunal mucosa identification?
S. Johnson
Verified customer
Asked: 2019-10-15
Answer
It shows on the product datasheet, PA1302-1 anti-Caspase-3 (P10)/CASP3 antibody has been validated for IHC-P, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human jejunal mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-15
Question
Can you help my question with product PA1302-1, anti-Caspase-3 (P10)/CASP3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1302-1 anti-Caspase-3 (P10)/CASP3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-04
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for jejunal mucosa using anti-Caspase-3 (P10)/CASP3 antibody PA1302-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-03
Question
Will anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 work for ICC with jejunal mucosa?
K. Miller
Verified customer
Asked: 2018-10-31
Answer
According to the expression profile of jejunal mucosa, CASP3 is highly expressed in jejunal mucosa. So, it is likely that anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 will work for ICC with jejunal mucosa.
Boster Scientific Support
Answered: 2018-10-31
Question
We are currently using anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
The anti-Caspase-3 (P10)/CASP3 antibody (PA1302-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-12
Question
Is this PA1302-1 anti-Caspase-3 (P10)/CASP3 antibody reactive to the isotypes of CASP3?
F. Roberts
Verified customer
Asked: 2017-07-25
Answer
The immunogen of PA1302-1 anti-Caspase-3 (P10)/CASP3 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-3(P10)(220-236aa CAMLKQYADKLEFMHIL). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-25
Question
I was wanting to use using your anti-Caspase-3 (P10)/CASP3 antibody for response to glucose studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
V. Zhao
Verified customer
Asked: 2016-12-02
Answer
Thank you for your inquiry. This PA1302-1 anti-Caspase-3 (P10)/CASP3 antibody is validated on intestinal cancer tissue, hela cell lysate, smmc cell lysate, lung cancer tissue. It is guaranteed to work for IHC-P, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-12-02
Question
Would PA1302-1 anti-Caspase-3 (P10)/CASP3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Baker
Verified customer
Asked: 2015-04-15
Answer
It shows on the product datasheet, PA1302-1 anti-Caspase-3 (P10)/CASP3 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-04-15
Question
See below the WB image, lot number and protocol we used for jejunal mucosa using anti-Caspase-3 (P10)/CASP3 antibody PA1302-1. Please let me know if you require anything else.
G. Jones
Verified customer
Asked: 2015-01-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-12
Question
Is there a BSA free version of anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 available?
C. Gonzalez
Verified customer
Asked: 2013-12-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-12-04
Question
Will anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 work on dog ICC with tongue?
M. Li
Verified customer
Asked: 2013-11-05
Answer
Our lab technicians have not tested anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Caspase-3 (P10)/CASP3 antibody PA1302-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog tongue in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-05
Question
We ordered your anti-Caspase-3 (P10)/CASP3 antibody for WB on jejunal mucosa in the past. I am using human, and I plan to use the antibody for ICC next. I am interested in examining jejunal mucosa as well as t-cell in our next experiment. Could give a recommendation on which antibody would work the best for ICC?
P. Jones
Verified customer
Asked: 2013-09-23
Answer
I looked at the website and datasheets of our anti-Caspase-3 (P10)/CASP3 antibody and it appears that PA1302-1 has been tested on human in both WB and ICC. Thus PA1302-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-09-23
Question
My boss were happy with the WB result of your anti-Caspase-3 (P10)/CASP3 antibody. However we have been able to see positive staining in cervix carcinoma erythroleukemia cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP3 supposed to be expressed?
T. Lewis
Verified customer
Asked: 2013-07-04
Answer
Based on literature, cervix carcinoma erythroleukemia does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-07-04
