Anti-Caspase-3 (P10)/CASP3 Antibody

Rating:
100% of 100

SKU PA1302-1
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC-P, ICC, WB

Overview

Product Name Anti-Caspase-3 (P10)/CASP3 Antibody
SKU/Catalog Number PA1302-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Caspase-3(CASP3) detection. Tested with WB, IHC-P, ICC in Human.
Cite This Product Anti-Caspase-3 (P10)/CASP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1302-1)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-3(P10)(220-236aa CAMLKQYADKLEFMHIL).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Images And Assay Conditions

Anti-Caspase-3(P10), PA1302-1, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Anti-Caspase-3(P10), PA1302-1, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate

Anti-Caspase-3(P10), PA1302-1, IHC(P)
IHC(P): Human Lung Cancer Tissue

Figure 4. ICC analysis of Caspase-3(P10) using anti-Caspase-3(P10) antibody (PA1302-1).
Caspase-3(P10) was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Caspase-3(P10) Antibody (PA1302-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P42574
Gene Name CASP3
Protein Name Caspase-3
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Alternative Names Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1;Caspase-3 subunit p17;Caspase-3 subunit p12;CASP3;CPP32;
Subcellular Localization Cytoplasm.
Molecular Weight 31608 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. .
Research Areas Human

*You can search these to find other products in these research areas.
Background Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Polyclonal antibody for CASPASE 3/CASP3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CASPASE 3/CASP3 information: Molecular Weight: 31608 MW; Subcellular Localization: Cytoplasm; Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
In stock
Order Product
PA1302-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting_box_image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Publications

miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit
Antitumor effects of Laminaria extract fucoxanthin on lung cancer
Recombinant Newcastle disease virus expressing human IFN%u2010%u03BB1 (rL%u2010hIFN%u2010%u03BB1)%u2010induced apoptosis of A549 cells is connected to endoplasmic reticulum stress %u2026
Maternal separation exacerbates Alzheimer's disease-like behavioral and pathological changes in adult APPswe/PS1dE9 mice
MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/%u03B2-catenin signaling
In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line
Inhibitory effects of vitamin E on osteocyte apoptosis and DNA oxidative damage in bone marrow hemopoietic cells at early stage of steroid-induced femoral %u2026
Myricetin exhibits anti-glioma potential by inducing mitochondrial-mediated apoptosis, cell cycle arrest, inhibition of cell migration and ROS generation
Copper ion attenuated the antiproliferative activity of di-2-pyridylhydrazone dithiocarbamate derivative; however, there was a lack of correlation between ROS %u2026
Reactive oxygen species mediate isoalantolactone-induced apoptosis in human prostate cancer cells
Diabetes Inhibits Cerebral Ischemia-Induced Astrocyte Activation - an Observation in the Cingulate Cortex
Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils
Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure
Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir
A novel fibroblast growth factor receptor 1 inhibitor protects against cartilage degradation in a murine model of osteoarthritis
Optimal concentration and time window for proliferation and differentiation of neural stem cells from embryonic cerebral cortex: 5% oxygen preconditioning for 72 hours
Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning
Liraglutide prevents fast weight gain and ?-cell dysfunction in male catch-up growth rats
Nutlin-3 downregulates p53 phosphorylation on serine392and induces apoptosis in hepatocellular carcinoma cells
In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures
Enhanced anti-apoptosis and gut epithelium protection function of acidic fibroblast growth factor after cancelling of its mitogenic activity
Heme oxygenase-1 induction by hemin protects liver cells from ischemia/reperfusion injury in cirrhotic rats
Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells
Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway
The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia
HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma
Nerve growth factor reduces apoptotic cell death in rat facial motor neurons after facial nerve injury
Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction
In vivo?and?in vitro?evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer
Powerful inner/outer controlled multi-target magnetic nanoparticle drug carrier prepared by liquid photo-immobilization
Qi QM, Xue YC, Lv J, Sun D, Du JX, Cai SQ, Li YH, Gu TC, Wang MB. Oncol Lett. 2018 May;15(5):6400-6408. doi: 10.3892/ol.2018.8177. Epub 2018 Mar 5. Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochond...
An J, Liu T, She R, Wu Q, Tian J, Shi R, Hao W, Ren X, Yang Y, Lu Y, Yang Y, Wu Y. Oncotarget. 2017 Dec 17;9(4):4475-4484. doi: 10.18632/oncotarget.23381. eCollection 2018 Jan 12. Replication of hepatitis E virus in the ovary and promotion of oocy...
Sun X, Cui M, Wang D, Guo B, Zhang L. Oncol Lett. 2018 Apr;15(4):5744-5750. doi: 10.3892/ol.2018.8071. Epub 2018 Feb 16. Tumor necrosis factor-related apoptosis inducing ligand overexpression and Taxol treatment suppresses the growth of cervical c...
Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...
Qian C,, Gong L, Yang Z, Chen W, Chen Y, Xu Z, Wu B, Tang C, Gao F, Zeng W,. Bmc Cardiovasc Disord. 2015 Jun 26;15:59. Doi: 10.1186/S12872-015-0046-9. Diastolic Dysfunction In Spontaneous Type 2 Diabetes Rhesus Monkeys: A Study Using Echocardiogra...
Feng H, Yin Sh, Tang Az, Tan Sh. Neurochem Res. 2011 Jun;36(6):1108-15. Doi: 10.1007/S11064-011-0455-9. Epub 2011 Mar 31. Salicylate Initiates Apoptosis In The Spiral Ganglion Neuron Of Guinea Pig Cochlea By Activating Caspase-3.
Liang J, Chen H, Pan W, Xu C. Mol Med Rep. 2012 Jun;5(6):1419-22. Doi: 10.3892/Mmr.2012.854. Epub 2012 Apr 2. Puerarin Inhibits Caspase-3 Expression In Osteoblasts Of Diabetic Rats.
Jia Y, Lin J, Mi Y, Zhang C. Reprod Toxicol. 2011 May;31(4):477-85. Doi: 10.1016/J.Reprotox.2010.12.057. Epub 2011 Jan 22. Quercetin Attenuates Cadmium-Induced Oxidative Damage And Apoptosis In Granulosa Cells From Chicken Ovarian Follicles.
Zhang T, Su J, Wang K, Zhu T, Li X. Neurosci Lett. 2014 Sep 5;579:12-7. Doi: 10.1016/J.Neulet.2014.07.005. Epub 2014 Jul 12. Ursolic Acid Reduces Oxidative Stress To Alleviate Early Brain Injury Following Experimental Subarachnoid Hemorrhage.
Li Ys, Chen Bf, Li Xj, Zhang Wk, Tang Hb. Plos One. 2014 Sep 24;9(9):E107361. Doi: 10.1371/Journal.Pone.0107361. Ecollection 2014. Cytotoxicity Of Polyaniline Nanomaterial On Rat Celiac Macrophages In Vitro.
Zhang L, Yu S, Duan Z, Wang Q, Tian G, Tian Y, Zhao W, Wang H, Zhang C, Guo S, Liu Q, He G, Bian T, Chang J, Jin X, Cui D. Int J Mol Med. 2014 Jan;33(1):117-27. Doi: 10.3892/Ijmm.2013.1542. Epub 2013 Oct 30. Treatment Of Liver Cancer In Mice By Th...
Hu K, Xie Yy, Zhang C, Ouyang Ds, Long Hy, Sun Dn, Long Ll, Feng L, Li Y, Xiao B. Bmc Neurosci. 2012 Sep 22;13:115. Doi: 10.1186/1471-2202-13-115. Microrna Expression Profile Of The Hippocampus In A Rat Model Of Temporal Lobe Epilepsy And Mir-34A-...
Liu Nb, Peng T, Pan C, Yao Yy, Shen B, Leng J. World J Gastroenterol. 2005 Oct 28;11(40):6281-7. Overexpression Of Cyclooxygenase-2 In Human Hepg2, Bel-7402 And Smmc-7721 Hepatoma Cell Lines And Mechanism Of Cyclooxygenase-2 Selective Inhibitor Ce...
Li Z, Shao S, Xie S, Jiao F, Ma Y, Shi S. Ir J Med Sci. 2010 Jun;179(2):217-23. Doi: 10.1007/S11845-009-0418-1. Epub 2009 Dec 20. Silencing Of Ct120 By Antisense Oligonucleotides Could Inhibit The Lung Cancer Cells Growth.
Feng H, Yin Sh, Tang Az, Cai Hw, Chen P, Tan Sh, Xie Lh. Neurosci Lett. 2010 Jul 19;479(1):34-9. Doi: 10.1016/J.Neulet.2010.05.023. Epub 2010 May 15. Caspase-3 Activation In The Guinea Pig Cochlea Exposed To Salicylate.
Zhang S, Li H, Yang Sj. Acta Pharmacol Sin. 2010 Jun;31(6):671-8. Doi: 10.1038/Aps.2010.45. Epub 2010 May 10. Tribulosin Protects Rat Hearts From Ischemia/Reperfusion Injury.
Sun Mm, Li Jf, Guo Ll, Xiao Ht, Dong L, Wang F, Huang Fb, Cao D, Qin T, Yin Xh, Li Jm, Wang Sl. Oncogene. 2014 Apr 17;33(16):2075-86. Doi: 10.1038/Onc.2013.165. Epub 2013 May 13. Tgf-??1 Suppression Of Microrna-450B-5P Expression: A Novel Mechanis...
Oztanir Mn, Ciftci O, Cetin A, Aladag Ma. Neurol Sci. 2014 Sep;35(9):1393-9. Doi: 10.1007/S10072-014-1725-5. Epub 2014 Mar 28. Hesperidin Attenuates Oxidative And Neuronal Damage Caused By Global Cerebral Ischemia/Reperfusion In A C57Bl/J6 Mouse M...
Ciftci O, Oztanir Mn, Cetin A. Neurochem Res. 2014 Sep;39(9):1717-23. Doi: 10.1007/S11064-014-1365-4. Epub 2014 Jun 28. Neuroprotective Effects Of ??-Myrcene Following Global Cerebral Ischemia/Reperfusion-Mediated Oxidative And Neuronal Damage In ...
Chen Y, Li D, Xu Y, Zhang Y, Tao L, Li S, Jiang Y, Shen X. Evid Based Complement Alternat Med. 2014;2014:956824. Doi: 10.1155/2014/956824. Epub 2014 Dec 23. Essential Oils From Fructus A. Zerumbet Protect Human Aortic Endothelial Cells From Apopto...
Zhou X, Zhang Y, Li Y, Hao X, Liu X, Wang Y. Cancers (Basel). 2012 Dec 4;4(4):1318-32. Doi: 10.3390/Cancers4041318. Azithromycin Synergistically Enhances Anti-Proliferative Activity Of Vincristine In Cervical And Gastric Cancer Cells.
Li Q, Li Z, Xu Xy, Guo Yl, Du F. Int J Mol Sci. 2010 Nov 16;11(11):4580-90. Doi: 10.3390/Ijms11114580. Neuroprotective Properties Of Picroside Ii In A Rat Model Of Focal Cerebral Ischemia.
Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.
Shu Hf, Yu Sx, Zhang Cq, Liu Sy, Wu Kf, Zang Zl, Yang H, Zhou Sw, Yin Q. Brain Dev. 2013 Mar;35(3):252-60. Doi: 10.1016/J.Braindev.2012.04.007. Epub 2012 May 28. Expression Of Trpv1 In Cortical Lesions From Patients With Tuberous Sclerosis Complex...
Wang Xf, Qu Xq, Zhang Tt, Zhang Jf. Exp Ther Med. 2015 Apr;9(4):1283-1291. Epub 2015 Feb 6. Testosterone Suppresses Ventricular Remodeling And Improves Left Ventricular Function In Rats Following Myocardial Infarction.
Li M, Peng J, Song Y, Liang H, Mei Y, Fang Y. J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):746-9. Doi: 10.1007/S11596-012-1028-0. Epub 2012 Oct 18. Electro-Acupuncture Combined With Transcranial Magnetic Stimulation Improves Learning And ...
Wu Z, Wang L, Hou S. Neural Regen Res. 2012 Jul 5;7(19):1493-7. Doi: 10.3969/J.Issn.1673-5374.2012.19.008. Alpha B-Crystallin Improved Survival Of Retinal Ganglion Cells In A Rat Model Of Acute Ocular Hypertension.
Chen A, Dang T, Wang S, Tang N, Liu Y, Wu W. Sci China Life Sci. 2014 Jul;57(7):698-709. Doi: 10.1007/S11427-014-4680-8. Epub 2014 Jun 16. The In Vitro And In Vivo Anti-Tumor Effects Of Mtx-Fe3O 4-Plla-Peg-Plla Microspheres Prepared By Suspension-...
Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L. Neural Regen Res. 2014 Apr 15;9(8):837-44. Doi: 10.4103/1673-5374.131600. Apoptosis Is An Obstacle To The Differentiation Of Adipose-Derived Stromal Cells Into Astrocytes.
Chen S, Peng H, Rowat A, Gao F, Zhang Z, Wang P, Zhang W, Wang X, Qu L. Brain Res. 2015 Aug 27;1618:205-11. Doi: 10.1016/J.Brainres.2015.05.027. Epub 2015 May 30. The Effect Of Concentration And Duration Of Normobaric Oxygen In Reducing Caspase-3 ...
Dai C, Li J, Tang S, Li J, Xiao X. Antimicrob Agents Chemother. 2014 Jul;58(7):4075-85. Doi: 10.1128/Aac.00070-14. Epub 2014 May 5. Colistin-Induced Nephrotoxicity In Mice Involves The Mitochondrial, Death Receptor, And Endoplasmic Reticulum Pathw...
Ji J, Yan X, Li Z, Lai Z, Liu J. Biomed Pharmacother. 2015 Feb;69:311-6. Doi: 10.1016/J.Biopha.2014.12.020. Epub 2014 Dec 19. Therapeutic Effects Of Intrathecal Versus Intravenous Monosialoganglioside Against Bupivacaine-Induced Spinal Neurotoxici...
Lin Hd, He Cq, Luo Ql, Zhang Jl, Zeng Dx. Rheumatol Int. 2012 Mar;32(3):759-66. Doi: 10.1007/S00296-010-1629-5. Epub 2010 Dec 28. The Effect Of Low-Level Laser To Apoptosis Of Chondrocyte And Caspases Expression, Including Caspase-8 And Caspase-3 ...
Guo H, Luo Q, Zhang J, Lin H, Xia L, He C. Joint Bone Spine. 2011 Dec;78(6):604-10. Doi: 10.1016/J.Jbspin.2011.01.009. Epub 2011 Mar 11. Comparing Different Physical Factors On Serum Tnf-?? Levels, Chondrocyte Apoptosis, Caspase-3 And Caspase-8 Ex...
Zeng D, Luo Q, Lin H, Zhang J, He C. Rheumatol Int. 2012 Dec;32(12):3771-7. Doi: 10.1007/S00296-011-2196-0. Epub 2011 Dec 14. The Effect Of Therapeutic Ultrasound To Apoptosis Of Chondrocyte And Caspase-3 And Caspase-8 Expression In Rabbit Surgery...
Shan S, Li Z, Newton Ip, Zhao C, Li Z, Guo M. Toxicol Lett. 2014 Jun 5;227(2):129-38. Doi: 10.1016/J.Toxlet.2014.03.008. Epub 2014 Mar 28. A Novel Protein Extracted From Foxtail Millet Bran Displays Anti-Carcinogenic Effects In Human Colon Cancer ...
Zhao X, Chen Q, Li Y, Tang H, Liu W, Yang X. Eur J Pharm Biopharm. 2015 Jun;93:27-36. Doi: 10.1016/J.Ejpb.2015.03.003. Epub 2015 Mar 11. Doxorubicin And Curcumin Co-Delivery By Lipid Nanoparticles For Enhanced Treatment Of Diethylnitrosamine-Induc...
Lin S, Lin K, Li W, Zhou X, Huang T. Endocrine. 2010 Jun;37(3):460-6. Doi: 10.1007/S12020-010-9337-1. Epub 2010 Apr 21. Maternal Diabetes Increases Apoptosis In Mice Oocytes, Not 2-Cell Embryos.
Du F, Ding Y, Zou J, Li Z, Tian J, She R, Wang D, Wang H, Lv D, Chang L. Plos One. 2015 May 22;10(5):E0127047. Doi: 10.1371/Journal.Pone.0127047. Ecollection 2015. Morphology And Molecular Mechanisms Of Hepatic Injury In Rats Under Simulated Weigh...
Li Tt, Zhang Jf, Fei Sj, Zhu Sp, Zhu Jz, Qiao X, Liu Zb. Acta Pharmacol Sin. 2014 Feb;35(2):185-94. Doi: 10.1038/Aps.2013.140. Epub 2013 Dec 23. Glutamate Microinjection Into The Hypothalamic Paraventricular Nucleus Attenuates Ulcerative Colitis I...
Li Wz, Li Wp, Huang Dk, Kan Hw, Wang X, Wu Wy, Yin Yy, Yao Yy. Behav Brain Res. 2012 Feb 1;227(1):142-9. Doi: 10.1016/J.Bbr.2011.10.038. Epub 2011 Oct 29. Dexamethasone And A????????-?????? Accelerate Learning And Memory Impairments Due To Elevate...
Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...
Chu Sh, Ma Yb, Feng Df, Zhang H, Qiu Jh, Zhu Za. Oncol Lett. 2011 Nov;2(6):1139-1142. Epub 2011 Aug 4. Elevated Expression Of Solute Carrier Family 22 Member 18 Increases The Sensitivity Of U251 Glioma Cells To Bcnu.
Zhao B, Li X. Oncol Rep. 2014 Jun;31(6):2769-75. Doi: 10.3892/Or.2014.3126. Epub 2014 Apr 3. Altholactone Induces Reactive Oxygen Species-Mediated Apoptosis In Bladder Cancer T24 Cells Through Mitochondrial Dysfunction, Mapk-P38 Activation And Akt...
Zhan W, Hu X, Yi J, An Q, Huang X. Mol Med Rep. 2015 Jun;11(6):4142-8. Doi: 10.3892/Mmr.2015.3326. Epub 2015 Feb 10. Inhibitory Activity Of Apogossypol In Human Prostate Cancer In Vitro And In Vivo.
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
Wang Jm, Xiao Bl, Zheng Jw, Chen Hb, Zou Sq. World J Gastroenterol. 2007 Jun 21;13(23):3171-5. Effect Of Targeted Magnetic Nanoparticles Containing 5-Fu On Expression Of Bcl-2, Bax And Caspase 3 In Nude Mice With Transplanted Human Liver Cancer.
Meng Qt, Xia Zy, Luo T, Cao C, Zhao B, Wu Y, Wu X, Chen X. Saudi Med J. 2012 Feb;33(2):139-45. Ligustrazine Attenuates Acute Lung Injury Induced By Blunt Chest Trauma.
Zhou Y, Li W, Xu Q, Huang Y. J Exp Clin Cancer Res. 2010 Oct 4;29:131. Doi: 10.1186/1756-9966-29-131. Elevated Expression Of Dickkopf-1 Increases The Sensitivity Of Human Glioma Cell Line Shg44 To Bcnu.
Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Int J Oncol. 2012 Apr;40(4):1153-61. Doi: 10.3892/Ijo.2011.1277. Epub 2011 Nov 30. Magnolol, A Natural Compound, Induces Apoptosis Of Sgc-7901 Human Gastric Adenocarcinoma Cells Vi...
Peng X, Chen K, Chen J, Fang J, Cui H, Zuo Z, Deng J, Chen Z, Geng Y, Lai W. Environ Toxicol. 2015 Mar 4. Doi: 10.1002/Tox.22120. [Epub Ahead Of Print] Aflatoxin B1 Affects Apoptosis And Expression Of Bax, Bcl-2, And Caspase-3 In Thymus And Bursa ...
Chen J, Li X, Qiu J, You H, Zhang Q, Dong G, Zuo Y. Cell Biochem Biophys. 2013;67(3):1015-9. Doi: 10.1007/S12013-013-9597-5. Kinetics Of Apoptosis And Expression Of Apoptosis-Related Proteins In Rat Ca3 Hippocampus Cells After Experimental Diffuse...
Liu Y, Zhou Y, Wang Y, Geng D, Liu J. Obes Surg. 2011 Sep;21(9):1424-31. Doi: 10.1007/S11695-011-0388-Z. Roux-En-Y Gastric Bypass-Induced Improvement Of Glucose Tolerance And Insulin Resistance In Type 2 Diabetic Rats Are Mediated By Glucagon-Like...
Yang Y, Li Zh, Liu H, Shi Wd, Zhang J. Chin J Integr Med. 2015 Jun;21(6):423-30. Doi: 10.1007/S11655-014-1752-3. Epub 2014 May 10. Inhibitory Effect Of Tetramethylpyrazine Preconditioning On Overload Training-Induced Myocardial Apoptosis In Rats.
Song Gh, Wang Rl, Chen Zy, Zhang B, Wang Hl, Liu Ml, Gao Jp, Yan Xy. J Physiol Biochem. 2014 Sep;70(3):761-8. Doi: 10.1007/S13105-014-0344-1. Epub 2014 Jul 30. Toxic Effects Of Sodium Fluoride On Cell Proliferation And Apoptosis Of Leydig Cells Fr...
Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J, Geng Y, Lai W. Food Chem Toxicol. 2013 Sep;59:446-54. Doi: 10.1016/J.Fct.2013.06.032. Epub 2013 Jun 28. Protective Role Of Sodium Selenite On Histopathological Lesions, D...
Mao Z, Song Z, Li G, Lv W, Zhao X, Li B, Feng X, Chen Y. Neural Regen Res. 2013 Jan 15;8(2):133-42. Doi: 10.3969/J.Issn.1673-5374.2013.02.005. 8-Hydroxy-2-(Di-N-Propylamino)Tetralin Intervenes With Neural Cell Apoptosis Following Diffuse Axonal In...
Yan Y, Liang B, Zhang J, Liu Y, Bu X. Mol Med Rep. 2015 Jan;11(1):317-26. Doi: 10.3892/Mmr.2014.2657. Epub 2014 Oct 15. Apoptotic Induction Of Lung Adenocarcinoma A549 Cells Infected By Recombinant Rvg Newcastle Disease Virus (Rl-Rvg) In Vitro.
Li X, Wang L, Wang Z. Phys Med. 2015 Jun;31(4):352-9. Doi: 10.1016/J.Ejmp.2015.02.004. Epub 2015 Feb 19. Radioprotective Activity Of Neutral Polysaccharides Isolated From The Fruiting Bodies Of Hohenbuehelia Serotina.
Wei T, Tian W, Yan H, Shao G, Xie G. Cell Mol Neurobiol. 2014 Nov;34(8):1165-73. Doi: 10.1007/S10571-014-0091-4. Epub 2014 Aug 22. Protective Effects Of Phillyrin On H2O 2-Induced Oxidative Stress And Apoptosis In Pc12 Cells.
Ding H, Wang H, Zhao Y, Sun D, Zhai X. Cell Mol Neurobiol. 2015 Jul;35(5):623-32. Doi: 10.1007/S10571-015-0156-Z. Epub 2015 Jan 18. Protective Effects Of Baicalin On A??????????????-Induced Learning And Memory Deficit, Oxidative Stress, And Apopto...
Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...
Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...
Tian W, Wei T, Li B, Wang Z, Zhang N, Xie G. Plos One. 2014 May 6;9(5):E96775. Doi: 10.1371/Journal.Pone.0096775. Ecollection 2014. Pathway Of Programmed Cell Death And Oxidative Stress Induced By ??-Hydroxybutyrate In Dairy Cow Abomasum Smooth Mu...
Dong Hy, Wang Zf. Chin J Cancer Res. 2014 Apr;26(2):200-7. Doi: 10.3978/J.Issn.1000-9604.2014.04.07. Antitumor Effects Of Artesunate On Human Breast Carcinoma Mcf-7 Cells And Igf-Ir Expression In Nude Mice Xenografts.
Huang Kf, Huang Xp, Xiao Gq, Yang Hy, Lin Js, Diao Y. Biomed Pharmacother. 2014 May;68(4):455-61. Doi: 10.1016/J.Biopha.2014.03.005. Epub 2014 Mar 18. Kallistatin, A Novel Anti-Angiogenesis Agent, Inhibits Angiogenesis Via Inhibition Of The Nf-??b...
Sun Y, Yang J, Wang Lz, Sun Lr, Dong Q. Hum Exp Toxicol. 2014 Aug;33(8):855-62. Doi: 10.1177/0960327113511475. Epub 2013 Nov 25. Crocin Attenuates Cisplatin-Induced Liver Injury In The Mice.
Fu Y, Zhao Z, Wu Y, Wu K, Xu X, Liu Y, Tong C. J Ethnopharmacol. 2012 Jan 6;139(1):26-33. Doi: 10.1016/J.Jep.2011.08.077. Epub 2011 Nov 10. Therapeutic Mechanisms Of Tongmai Dasheng Tablet On Tripterygium Glycosides Induced Rat Model For Premature...
Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.
Xia L, Luo Ql, Lin Hd, Zhang Jl, Guo H, He Cq. Rheumatol Int. 2012 Sep;32(9):2847-56. Doi: 10.1007/S00296-011-2080-Y. Epub 2011 Sep 1. The Effect Of Different Treatment Time Of Millimeter Wave On Chondrocyte Apoptosis, Caspase-3, Caspase-8, And Mm...
Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.
Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.
Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...
Ran Yh, Wang H. J Zhejiang Univ Sci B. 2011 Oct;12(10):835-45. Doi: 10.1631/Jzus.B1100067. Iptakalim, An Atp-Sensitive Potassium Channel Opener, Confers Neuroprotection Against Cerebral Ischemia/Reperfusion Injury In Rats By Protecting Neurovascul...

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to caspase 3 antibody, casp3 antibody, caspase3 antibody
Write a review for PA1302-1