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CASP3 antibody (M00334-2) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 39 Publications

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Designing a Western blot for CASP3? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the CASP3 Western Blot Guide

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Anti-Caspase-3 p12 CASP3 Rabbit Monoclonal Antibody

CASP3 antibody

39 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IP, IF, IHC, ICC, WB

Tested Applications

CASP3 antibody was positively detected by WB in:

human HepG2 whole cell, human HEL whole cell, human RT4 whole cell, human SH-SY5Y whole cell, rat lung tissue, rat RH35 whole cell, mouse lung tissue, mouse HEPA1-6 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CASP3 antibody was positively detected by IHC in:

human kidney tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Caspase-3 p12 CASP3 Rabbit Monoclonal Antibody
View all CASP3 Antibodies

Form

Liquid

Uniprot

P42574

Gene ID

836

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

GEA-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Caspase-3 p12

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

35 kDa

Calculated Molecular Weight

31.6 kDa

Cite This Product

Anti-Caspase-3 p12 CASP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-2)

Protocols

Western Blot protocol for CASP3 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CASP3 antibody

Review Protocol

Publications

Effects of interstitial photodynamic therapy on transplanted tumors of human lung adenocarcinoma A549 cells in nude mice

Published: 2026-07-10

Journal: LASERS IN MEDICAL SCIENCE

Application: WB

Impact Factor: 2.70

Species: Mouse

PMID: 42429993

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Free Secondary Antibody

Anti-Caspase-3 p12 CASP3 Rabbit Monoclonal Antibody

CASP3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Effects of interstitial photodynamic therapy on transplanted tumors of human lung adenocarcinoma A549 cells in nude mice

Reactive oxygen species drive wound epithelium and blastema formation in regenerating tail of Scincella tsinlingensis

Histologic and immunohistochemical changes in amputated and scarring lizard limb of Scincella tsinlingensis

Exosome-like nanovesicles from Dunaliella salina Efficient Sequential Co-Delivery of anti-PDL1 and miR-375 for enhancing gene/immune therapy

Construction of ROS-responsive cascade-targeted liposomes and investigation of their anti-AD effects

The long noncoding RNA RMRP-miR-3135a-SV2A axis promotes the development of hepatocellular carcinoma

Ketone body 3-hydroxybutyrate mitigates apoptosis and enhances osteogenesis in bone organoid construction via the cAMP/PKA/CREB signaling pathway

IL-37 Alleviates Sepsis-Induced Lung Injury by Inhibiting Inflammatory Response Through the TGF-β/Smad3 Pathway

Tetrachlorantraniliprole induces neurodevelopmental toxicity through oxidative stress-mediated apoptosis and dysregulation of Wnt signaling pathway

Halcinonide activates smoothened to ameliorate ischemic stroke injury

Knockdown of NDUFAF6 inhibits breast cancer progression via promoting mitophagy and apoptosis

Taraxasterol attenuates zearalenone-induced kidney damage in mice by modulating oxidative stress and endoplasmic reticulum stress

Astaxanthin inhibits apoptosis in a cell model of tauopathy by attenuating endoplasmic reticulum stress and unfolded protein response

Delay the progression of glucocorticoid-induced osteoporosis: Fraxin targets ferroptosis via the Nrf2/GPX4 pathway

Structural characteristics and anti-tumor effect of low molecular weight Dendrobium officinale polysaccharides by reconstructing tumor microenvironment

Parathyroid hormone (1–34) retards the lumbar facet joint degeneration and activates Wnt/β-catenin signaling pathway in ovariectomized rats

The Study of SRSF1 Regulates Abnormal Alternative Splicing of BCL2L11 and the Role in Refractory Acute Myeloid Leukemia

SHARPIN contributes to sevoflurane-induced neonatal neurotoxicity through up-regulating HMGB1 to repress M2 like-macrophage polarization

Selective bioaccumulation of polystyrene nanoplastics in fetal rat brain and damage to myelin development

Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study

Effects of alendronate on cartilage lesions and micro-architecture deterioration of subchondral bone in patellofemoral osteoarthritic ovariectomized rats with patella-baja

Cytisine–N-methylene-(5,7,4’-trihydroxy)- isoflavone ameliorates ischemic stroke-induced brain injury in mouse by regulating the oxidative stress and BDNF-Trkb/Akt pathway

Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway

A breakthrough in periodontitis treatment: Revealing the pharmacodynamic substances and mechanisms of Kouqiangjie formula

Canagliflozin ameliorates high glucose-induced apoptosis in NRK-52E cells via inhibiting oxidative stress and activating AMPK/mTOR-mediated autophagy

(2E)-1-(2,4,6-Trimethoxyphenyl)-3-(4-chlorophenyl)prop-2-en-1-one, a Chalcone Derivative, Promotes Apoptosis by Suppressing RAS-ERK and AKT/FOXO3a Pathways in Hepatocellular Carcinoma Cells

Hsa_circ_0001550 impairs decidualization by regulating the proliferation and apoptosis of endometrial stromal cells

Tanshinone IIA inhibits cardiomyocyte apoptosis and rescues cardiac function during doxorubicin-induced cardiotoxicity by activating the DAXX/MEK/ERK1/2 pathway

Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways

Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells

Antagonistic effect of nano-selenium on hepatocyte apoptosis induced by DEHP via PI3K/AKT pathway in chicken liver

Emodin Ameliorates Renal Damage and Podocyte Injury in a Rat Model of Diabetic Nephropathy via Regulating AMPK/mTOR-Mediated Autophagy Signaling Pathway

Highly Tumor-Specific and Long-Acting Iodine-131 Microbeads for Enhanced Treatment of Hepatocellular Carcinoma with Low-Dose Radio-Chemoembolization

Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism

Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice

WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects

Liraglutide prevents fast weight gain and β-cell dysfunction in male catch-up growth rats

A novel protein extracted from foxtail millet bran displays anti-carcinogenic effects in human colon cancer cells

BMP9-Induced Osteogenetic Differentiation and Bone Formation of Muscle-Derived Stem Cells

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