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CASP3 antibody (M00334-5) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 27 Publications

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Immunofluorescent analysis of Hela cells, using Caspase-3 Antibody .

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Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody

CASP3 antibody

27 Publications

Reactive Species	Host	Application
Human	Rabbit	Flow Cytometry, IP, IF, IHC, ICC, WB

Tested Applications

CASP3 antibody was positively detected by WB in:

human Hela whole cell, human Hela whole cell, human Jurkat whole cell, human Jurkat whole cell, human HepG2 whole cell, human 293T whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CASP3 antibody was positively detected by IHC in:

human tonsil tissue, human tonsil tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CASP3 antibody was positively detected by ICC/IF in:

Hela cell

Reactive Data

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IHC

Human

Tested

Recommend

Product Information

Product Name

Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody
View all CASP3 Antibodies

Form

Liquid

Uniprot

P42574

Gene ID

836

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

AOE-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Caspase-3

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

32 kDa

Calculated Molecular Weight

31.6 kDa

Cite This Product

Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-5)

Protocols

Western Blot protocol for CASP3 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CASP3 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CASP3 antibody

Review Protocol

Publications

Echinococcus granulosus-Induced Liver Damage Through Ferroptosis in Rat Model

Published: 2025-02-22

Journal: Cells

Application: WB

Impact Factor: 5.20

Species: Rat

PMID: 40072057

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Free Secondary Antibody

Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody

CASP3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Echinococcus granulosus-Induced Liver Damage Through Ferroptosis in Rat Model

Saroglitazar ameliorates 5- Fluorouracil-induced hepatorenal damage in rats

MicroRNA-138 promotes the progression of multiple myeloma through targeting paired PAX5

Parathyroid hormone (1–34) retards the lumbar facet joint degeneration and activates Wnt/β-catenin signaling pathway in ovariectomized rats

“Butterfly-effect” nano-platforms for cascade immune amplification and tumor clearance through multi-modal therapy and immune sensitization

Effects of alendronate on cartilage lesions and micro-architecture deterioration of subchondral bone in patellofemoral osteoarthritic ovariectomized rats with patella-baja

HNF1A induces glioblastoma by upregulating EPS8 and activating PI3K/AKT signaling pathway

Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway

Rutaecarpine Ameliorates Murine N-Methyl-N’-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis by Sonic Hedgehog Pathway

Effects of Cooling Interventions with Different Target Temperatures on Heat Stroke Rats

Effect of Diammonium Glycyrrhizinate in Improving Focal Cerebral Ischemia-Reperfusion Injury in Rats Through Multiple Mechanisms

Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways

Tetrandrine attenuates hyperoxia-induced lung injury in newborn rats via NF-κB p65 and ERK1/2 pathway inhibition

Sonodynamic therapy in atherosclerosis by curcumin nanosuspensions: Preparation design, efficacy evaluation, and mechanisms analysis

TBX3 deficiency accelerates apoptosis in cardiomyoblasts through regulation of P21 expression

RETRACTED ARTICLE: MicroRNA-93 regulates the neurological function, cerebral edema and neuronal apoptosis of rats with intracerebral hemorrhage through TLR4/NF-κB signaling pathway

miR‑210‑3p regulates the proliferation and apoptosis of non‑small cell lung cancer cells by targeting SIN3A

Molecular mechanisms underlying nickel nanoparticle induced rat Sertoli-germ cells apoptosis

Polyamine analogue QMA attenuated ischemic injury in MCAO rats via ERK and Akt activated Nrf2/HO-1 signaling pathway

Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway

Estrogen and propofol combination therapy inhibits endoplasmic reticulum stress and remarkably attenuates cerebral ischemia-reperfusion injury and OGD injury in hippocampus

Protective effects of extracts of Schisandra chinensis stems against acetaminophen-induced hepatotoxicity via regulation of MAPK and caspase-3 signaling pathways

Hepatoprotective effect of flavonoid-enriched fraction from Cyclocarya paliurus leaves on LPS/D-GalN-induced acute liver failure

MiR-429 regulates rat liver regeneration and hepatocyte proliferation by targeting JUN/MYC/BCL2/CCND1 signaling pathway

Bone marrow mesenchymal stem cell-derived exosomal miR-21 protects C-kit+ cardiac stem cells from oxidative injury through the PTEN/PI3K/Akt axis

Neuroprotective Properties of Picroside II in a Rat Model of Focal Cerebral Ischemia

Effects of Kang-Jia-Wan, a Chinese medicinal herb officinal, on apoptosis induction in goiter of rats

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