Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody

Caspase-3 antibody

Boster Bio Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody catalog # M00334-5. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: M00334-5
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody

View all Caspase-3 Antibodies

SKU/Catalog Number

M00334-5

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody catalog # M00334-5. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-5)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOE-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Caspase-3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00334-5 is reactive to CASP3 in Human

Applications

M00334-5 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

32 kDa

Calculated molecular weight

31.608kDa

Background of Caspase-3

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CASP3 (Source: Uniprot.org, NCBI)

Gene Name

CASP3

Full Name

Caspase-3

Weight

31.608kDa

Superfamily

peptidase C14A family

Alternative Names

Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CASP3, check out the CASP3 Infographic

CASP3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CASP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00334-5 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
M00334-5 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:200

Gang Li,Jing Zhou,Mengyu Sun,Juren Cen,Jing Xu,Role of luteolin extracted from Clerodendrum cyrtophyllum Turcz leaves in protecting HepG2 cells from TBHP-induced oxidative stress and its cytotoxicity, genotoxicity,Journal of Functional Foods, Volume 74, 2
Species: Human
M00334-5 usage in article: APP:WB, SAMPLE:HEPG-2 CELL, DILUTION:1:2000

Yu Y,Wang Y,Fei X,Song Z,Xie F,Yang F,Liu X,Xu Z,Wang G.All-Trans Retinoic Acid Prevented Vein Grafts Stenosis by Inhibiting Rb-E2F Mediated Cell Cycle Progression and KLF5-RARα Interaction in Human Vein Smooth Muscle Cells.Cardiovasc Drugs Ther.2020 Oct
Species: Human,New Zealand White Rabbit
M00334-5 usage in article: APP:WB, SAMPLE:HUVMSCS, DILUTION:1:1000

Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
M00334-5 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:1000

Yue W,Cunlin G,Lu H,Yuanqing Z,Yanjun T,Qiong W. Neuroprotective effect of intermittent hypobaric hypoxia preconditioning on cerebral ischemia/reperfusion in rats. Int J Clin Exp Pathol.2020 Nov 1;13(11):2860-2869.PMID:33284899;PMCID:PMC7716138.
Species: Rat
M00334-5 usage in article: APP:WB, SAMPLE:NEURONS, DILUTION:1:1500

Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
M00334-5 usage in article: APP:WB, SAMPLE:A431 CELLS, DILUTION:NA

Maternal separation exacerbates Alzheimer's disease-like behavioral and pathological changes in adult APPswe/PS1dE9 mice

MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/%u03B2-catenin signaling

Diabetes Inhibits Cerebral Ischemia-Induced Astrocyte Activation - an Observation in the Cingulate Cortex

Optimal concentration and time window for proliferation and differentiation of neural stem cells from embryonic cerebral cortex: 5% oxygen preconditioning for 72 hours

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15 Customer Q&As for Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody

Question

Does anti-Caspase-3 Rabbit Monoclonal antibody M00334-5 work for IHC with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

According to the expression profile of cervix carcinoma erythroleukemia, CASP3 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Caspase-3 Rabbit Monoclonal antibody M00334-5 will work for IHC with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2020-04-23

Question

We are interested in to test anti-Caspase-3 Rabbit Monoclonal antibody M00334-5 on rat cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Caspase-3 Rabbit Monoclonal antibody M00334-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

The products we sell, including anti-Caspase-3 Rabbit Monoclonal antibody M00334-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-23

Question

We are currently using anti-Caspase-3 Rabbit Monoclonal antibody M00334-5 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

The anti-Caspase-3 Rabbit Monoclonal antibody (M00334-5) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-13

Question

Is a blocking peptide available for product anti-Caspase-3 Rabbit Monoclonal antibody (M00334-5)?

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

We do provide the blocking peptide for product anti-Caspase-3 Rabbit Monoclonal antibody (M00334-5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-07

Question

Will M00334-5 anti-Caspase-3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

As indicated on the product datasheet, M00334-5 anti-Caspase-3 Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-13

Question

Is there a BSA free version of anti-Caspase-3 Rabbit Monoclonal antibody M00334-5 available?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-3 Rabbit Monoclonal antibody M00334-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-01

Question

I was wanting to use your anti-Caspase-3 Rabbit Monoclonal antibody for IHC for rat cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

As indicated on the product datasheet, M00334-5 anti-Caspase-3 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-27

Question

I have a question about product M00334-5, anti-Caspase-3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00334-5 anti-Caspase-3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-26

Question

Is this M00334-5 anti-Caspase-3 Rabbit Monoclonal antibody reactive to the isotypes of CASP3?

S. Banerjee

Verified customer

Asked: 2019-05-20

Answer

The immunogen of M00334-5 anti-Caspase-3 Rabbit Monoclonal antibody is A synthesized peptide derived from human Caspase-3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-20

Question

We ordered your anti-Caspase-3 Rabbit Monoclonal antibody for IF on lymph in the past. I am using human, and We intend to use the antibody for WB next. My lab would like examining lymph as well as tongue in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-09-25

Answer

I have checked the website and datasheets of our anti-Caspase-3 Rabbit Monoclonal antibody and it appears that M00334-5 has been validated on human in both IF and WB. Thus M00334-5 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-09-25

Question

See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Caspase-3 Rabbit Monoclonal antibody M00334-5. Please let me know if you require anything else.

B. Patel

Verified customer

Asked: 2018-07-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-16

Question

We have observed staining in rat cervix carcinoma erythroleukemia. What should we do? Is anti-Caspase-3 Rabbit Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?

A. Carter

Verified customer

Asked: 2018-05-17

Answer

From literature cervix carcinoma erythroleukemia does express CASP3. From Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-05-17

Question

I see that the anti-Caspase-3 Rabbit Monoclonal antibody M00334-5 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-08-01

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-08-01

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Caspase-3 Rabbit Monoclonal antibody M00334-5. Let me know if you need anything else.

M. Wu

Verified customer

Asked: 2015-08-21

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-08-21

Question

My boss were content with the WB result of your anti-Caspase-3 Rabbit Monoclonal antibody. However we have seen positive staining in t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP3 supposed to be expressed?

B. Yang

Verified customer

Asked: 2014-07-28

Answer

Based on literature, t-cell does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2014-07-28

Order DetailsPrice
M00334-5

100μL

$370
M00334-5-30ul

30μL sample

$99
M00334-5-carrier-free

Carrier Free

$370

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M00334-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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