Skip to the beginning of the images gallery

CASP3 antibody (M00334-9) performs well in IP, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 35 Publications

Datasheet MSDS Request COA

m00334 9 casp3 primary antibodies ihc testing 2

View All Images (4)

IHC analysis of Caspase 3 using anti-Caspase 3 antibody (M00334-9).
Caspase 3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Caspase 3 Antibody (M00334-9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Designing a Western blot for CASP3? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the CASP3 Western Blot Guide

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-Caspase 3 Rabbit Monoclonal Antibody

CASP3 antibody

35 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IP, IHC, WB

Tested Applications

CASP3 antibody was positively detected by WB in:

Jurkat cell, NIH/3T3 cell, Rat brain, human Hela whole cell, human Hela whole cell, human Jurkat whole cell, human Jurkat whole cell, human HepG2 whole cell, human 293T whole cell, mouse NIH/3T3 whole cell, mouse NIH/3T3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CASP3 antibody was positively detected by IHC in:

human tonsil tissue, human tonsil tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Rat

Tested

Recommend

Human

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Caspase 3 Rabbit Monoclonal Antibody
View all CASP3 Antibodies

Form

Liquid

Uniprot

P42574

Gene ID

836

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

30C96

Isotype

IgG

Immunogen

A synthesized peptide derived from human Caspase 3

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

32 kDa

Calculated Molecular Weight

31.6 kDa

Cite This Product

Anti-Caspase 3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-9)

Protocols

Western Blot protocol for CASP3 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CASP3 antibody

Review Protocol

Publications

Astragaloside IV targets TUBB4B to inhibit proliferation and promote apoptosis of pituitary tumor cells via the STMN1/ERK pathway

Published: 2026-04-01

Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Application: WB

Impact Factor: 5.80

Species: Rat

PMID: 41930558

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-Caspase 3 Rabbit Monoclonal Antibody

CASP3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Astragaloside IV targets TUBB4B to inhibit proliferation and promote apoptosis of pituitary tumor cells via the STMN1/ERK pathway

Network Pharmacology and Untargeted Metabolomics Analysis of the Protective Mechanisms of Total Flavonoids from Chuju in Myocardial Ischemia-Reperfusion Injury

Promoting Drp1-Mediated Mitochondrial Division in Nickel Nanoparticles-Induced Reproductive Toxicity in GC-2 Cells

Investigation of the Effects of Saxagliptin in In Vitro and In Vivo Models of Diabetic Neuropathy

Smart biodegradable hydrogels incorporating a photoactive Ir(III) complex for NIR-triggered oral cancer treatment

Multi-omics analysis reveals classic decoction alleviates ulcerative colitis by modulating tryptophan metabolism, gut microbiota homeostasis, and mitochondrial Bax/Bcl-2 pathways

Preconditioning with acteoside ameliorates myocardial ischemia‑reperfusion injury by targeting HSP90AA1 and the PI3K/Akt signaling pathway

Parathyroid hormone (1–34) retards the lumbar facet joint degeneration and activates Wnt/β-catenin signaling pathway in ovariectomized rats

Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents

Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways

An experimental rat model of electric shock injury with isolated electric shock and water conduction: the histopathological changes on the skin and internal organs and the effect on biochemical parameters

MiR-153 downregulation alleviates PTSD-like behaviors and reduces cell apoptosis by upregulating the Sigma-1 receptor in the hippocampus of rats exposed to single-prolonged stress

Circular RNA Rbms1 inhibited the development of myocardial ischemia reperfusion injury by regulating miR-92a/BCL2L11 signaling pathway

Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line

CD4+T cells apoptosis and conversion of Th1/Th2-type cytokines promote the progress of sporotrichosis

Neuroprotective effect of intermittent hypobaric hypoxia preconditioning on cerebral ischemia/reperfusion in rats

Astragaloside IV Reduces Cerebral Ischemia/Reperfusion-Induced Blood-Brain Barrier Permeability in Rats by Inhibiting ER Stress-Mediated Apoptosis

All-Trans Retinoic Acid Prevented Vein Grafts Stenosis by Inhibiting Rb-E2F Mediated Cell Cycle Progression and KLF5-RARα Interaction in Human Vein Smooth Muscle Cells

Role of luteolin extracted from Clerodendrum cyrtophyllum Turcz leaves in protecting HepG2 cells from TBHP-induced oxidative stress and its cytotoxicity, genotoxicity

Inhibition Effect of Dictyophora Polysaccharides on Human Hepatocellular Carcinoma Cell Line HCC-LM3

SOX2-induced upregulation of lncRNA LINC01510 promotes papillary thyroid carcinoma progression by modulating miR-335/SHH and activating Hedgehog pathway

Paeonol protects mitochondrial injury and prevents pulmonary vascular remodeling in hypoxia

Protective Effect of Alendronate on Lumbar Facet Degeneration in Ovariectomized Rats

Inotodiol inhibits cells migration and invasion and induces apoptosis via p53-dependent pathway in HeLa cells

Roles of poly (ADP-ribose) polymerase 1 activation and cleavage in induction of multi-oocyte ovarian follicles in the mouse by 3-nitropropionic acid

Combination of BMSCs-laden acellular nerve xenografts transplantation and G-CSF administration promotes sciatic nerve regeneration

Selenium-Rich Diet Induces Myocardial Structural and Functional Abnormalities by Activating Caspase-9 and Caspase-3 in Gpx-1P198L-Overexpression Transgenic Mice

Perfluorooctanoic acid exposure during pregnancy alters the apoptosis of uterine cells in pregnant mice

The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways

Protective effects of extracts of Schisandra chinensis stems against acetaminophen-induced hepatotoxicity via regulation of MAPK and caspase-3 signaling pathways

Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis

Salmon Calcitonin Attenuates Degenerative Changes in Cartilage and Subchondral Bone in Lumbar Facet Joint in an Experimental Rat Model

Ginsenoside Rb1 inhibit apoptosis in rat model of Alzheimer’s disease induced by Aβ1-40

Intra-hydrogel culture prevents transformation of mesenchymal stem cells induced by monolayer expansion

Experimental study on the therapeutic effect and underlining mechanisms of positron in pancreatic cancer cells

Review

Have you used Anti-Caspase 3 Rabbit Monoclonal Antibody?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality