Anti-Caspase-8 (P18)/CASP8 Antibody

Boster Bio Anti-Caspase-8 (P18)/CASP8 Antibody catalog # PA1308. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 17 publication(s).

Product Info Summary

SKU: PA1308
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Caspase-8 (P18)/CASP8 Antibody

See all Caspase-8 products

SKU/Catalog Number

PA1308

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-8 (P18)/CASP8 Antibody catalog # PA1308. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-8 (P18)/CASP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1308)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Caspase-8 (P18) (240-259aa DKVYQMKSKPRGYCLIINNH), different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1308 is reactive to CASP8 in Human, Mouse, Rat

Applications

PA1308 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For CASP8 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q14790

Gene ID

841

Gene Name

CASP8

Full Name

Caspase-8

Weight

55.391kDa

Superfamily

peptidase C14A family

Alternative Names

AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CASP8, check out the CASP8 Infographic

CASP8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CASP8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1308 has been cited in 17 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhou L,Yu W,Jayabalan DS,Niesvizky R,Jaffrey SR,Huang X,Xu G.Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function.Front Cell Dev Biol.2020 Dec 17;8:605989.doi:10.3389/f
Species: Human
PA1308 usage in article: APP:WB, SAMPLE:HEK293 CELL AND HELA CELL, DILUTION:NA

Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (%u2212)-gossypol

Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL

Inhibitory activity of apogossypol in human prostate cancerin vitroandin vivo

Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir

Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (?)-gossypol

Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells

2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL

Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.

Lin Hd, He Cq, Luo Ql, Zhang Jl, Zeng Dx. Rheumatol Int. 2012 Mar;32(3):759-66. Doi: 10.1007/S00296-010-1629-5. Epub 2010 Dec 28. The Effect Of Low-Level Laser To Apoptosis Of Chondrocyte And Caspases Expression, Including Caspase-8 And Caspase-3 ...

Guo H, Luo Q, Zhang J, Lin H, Xia L, He C. Joint Bone Spine. 2011 Dec;78(6):604-10. Doi: 10.1016/J.Jbspin.2011.01.009. Epub 2011 Mar 11. Comparing Different Physical Factors On Serum Tnf-?? Levels, Chondrocyte Apoptosis, Caspase-3 And Caspase-8 Ex...

Zeng D, Luo Q, Lin H, Zhang J, He C. Rheumatol Int. 2012 Dec;32(12):3771-7. Doi: 10.1007/S00296-011-2196-0. Epub 2011 Dec 14. The Effect Of Therapeutic Ultrasound To Apoptosis Of Chondrocyte And Caspase-3 And Caspase-8 Expression In Rabbit Surgery...

Shan S, Li Z, Newton Ip, Zhao C, Li Z, Guo M. Toxicol Lett. 2014 Jun 5;227(2):129-38. Doi: 10.1016/J.Toxlet.2014.03.008. Epub 2014 Mar 28. A Novel Protein Extracted From Foxtail Millet Bran Displays Anti-Carcinogenic Effects In Human Colon Cancer ...

Zhan W, Hu X, Yi J, An Q, Huang X. Mol Med Rep. 2015 Jun;11(6):4142-8. Doi: 10.3892/Mmr.2015.3326. Epub 2015 Feb 10. Inhibitory Activity Of Apogossypol In Human Prostate Cancer In Vitro And In Vivo.

Yan Y, Liang B, Zhang J, Liu Y, Bu X. Mol Med Rep. 2015 Jan;11(1):317-26. Doi: 10.3892/Mmr.2014.2657. Epub 2014 Oct 15. Apoptotic Induction Of Lung Adenocarcinoma A549 Cells Infected By Recombinant Rvg Newcastle Disease Virus (Rl-Rvg) In Vitro.

Xia L, Luo Ql, Lin Hd, Zhang Jl, Guo H, He Cq. Rheumatol Int. 2012 Sep;32(9):2847-56. Doi: 10.1007/S00296-011-2080-Y. Epub 2011 Sep 1. The Effect Of Different Treatment Time Of Millimeter Wave On Chondrocyte Apoptosis, Caspase-3, Caspase-8, And Mm...

Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.

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16 Customer Q&As for Anti-Caspase-8 (P18)/CASP8 Antibody

Question

Is this PA1308 anti-Caspase-8 (P18)/CASP8 antibody reactive to the isotypes of CASP8?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

The immunogen of PA1308 anti-Caspase-8 (P18)/CASP8 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Caspase-8(P18)(240-259aa DKVYQMKSKPRGYCLIINNH), different from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-10

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Caspase-8 (P18)/CASP8 antibody PA1308. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-24

Question

We have observed staining in human leukocyte. Do you have any suggestions? Is anti-Caspase-8 (P18)/CASP8 antibody supposed to stain leukocyte positively?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

According to literature leukocyte does express CASP8. According to Uniprot.org, CASP8 is expressed in small intestine peyer's patch, b-cell thymus, t-cell, leukocyte, among other tissues. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496

Boster Scientific Support

Answered: 2019-12-30

Question

I have a question about product PA1308, anti-Caspase-8 (P18)/CASP8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1308 anti-Caspase-8 (P18)/CASP8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-14

Question

I was wanting to use your anti-Caspase-8 (P18)/CASP8 antibody for WB for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

You can see on the product datasheet, PA1308 anti-Caspase-8 (P18)/CASP8 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-30

Question

Is a blocking peptide available for product anti-Caspase-8 (P18)/CASP8 antibody (PA1308)?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

We do provide the blocking peptide for product anti-Caspase-8 (P18)/CASP8 antibody (PA1308). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-23

Question

I see that the anti-Caspase-8 (P18)/CASP8 antibody PA1308 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-25

Question

Does PA1308 anti-Caspase-8 (P18)/CASP8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Jha

Verified customer

Asked: 2018-12-18

Answer

As indicated on the product datasheet, PA1308 anti-Caspase-8 (P18)/CASP8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-18

Question

Here is the WB image, lot number and protocol we used for t-cell using anti-Caspase-8 (P18)/CASP8 antibody PA1308. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-03-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-03-12

Question

Does anti-Caspase-8 (P18)/CASP8 antibody PA1308 work for WB with t-cell?

Verified Customer

Verified customer

Asked: 2017-10-16

Answer

According to the expression profile of t-cell, CASP8 is highly expressed in t-cell. So, it is likely that anti-Caspase-8 (P18)/CASP8 antibody PA1308 will work for WB with t-cell.

Boster Scientific Support

Answered: 2017-10-16

Question

Our lab were happy with the WB result of your anti-Caspase-8 (P18)/CASP8 antibody. However we have observed positive staining in t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP8 supposed to be expressed?

J. Banerjee

Verified customer

Asked: 2017-06-27

Answer

From what I have seen in literature, t-cell does express CASP8. Generally CASP8 expresses in cytoplasm. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496

Boster Scientific Support

Answered: 2017-06-27

Question

I am interested in using your anti-Caspase-8 (P18)/CASP8 antibody for apoptotic cleavage of cellular proteins studies. Has this antibody been tested with western blotting on liver tissue? We would like to see some validation images before ordering.

R. Johnson

Verified customer

Asked: 2015-11-09

Answer

Thanks for your inquiry. This PA1308 anti-Caspase-8 (P18)/CASP8 antibody is tested on rat thymus tissue, tissue lysate, liver tissue, hela whole cell lysate, smmc whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-11-09

Question

Will anti-Caspase-8 (P18)/CASP8 antibody PA1308 work on feline WB with b-cell thymus?

J. Jones

Verified customer

Asked: 2015-05-25

Answer

Our lab technicians have not tested anti-Caspase-8 (P18)/CASP8 antibody PA1308 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Caspase-8 (P18)/CASP8 antibody PA1308 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline b-cell thymus in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-05-25

Question

Is there a BSA free version of anti-Caspase-8 (P18)/CASP8 antibody PA1308 available?

P. Miller

Verified customer

Asked: 2014-06-02

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-8 (P18)/CASP8 antibody PA1308 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-06-02

Question

We need to test anti-Caspase-8 (P18)/CASP8 antibody PA1308 on human t-cell for research purposes, then I may be interested in using anti-Caspase-8 (P18)/CASP8 antibody PA1308 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

R. Krishna

Verified customer

Asked: 2013-05-03

Answer

The products we sell, including anti-Caspase-8 (P18)/CASP8 antibody PA1308, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-05-03

Question

We are currently using anti-Caspase-8 (P18)/CASP8 antibody PA1308 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

V. Williams

Verified customer

Asked: 2013-04-03

Answer

The anti-Caspase-8 (P18)/CASP8 antibody (PA1308) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-04-03

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PA1308
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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