Anti-Caspase-8(p18)/CASP8 Antibody

Boster Bio Anti-Caspase-8 (p18)/CASP8 Antibody catalog # PA1735. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 16 publication(s).

Product Info Summary

SKU: PA1735
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Caspase-8(p18)/CASP8 Antibody

See all Caspase-8 products

SKU/Catalog Number

PA1735

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-8 (p18)/CASP8 Antibody catalog # PA1735. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-8(p18)/CASP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1735)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-8 (p18) (257-277aa NNHNFAKAREKVPKLHSIRDR).

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1735 is reactive to CASP8 in Human

Applications

PA1735 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CASP8 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q14790

Gene ID

841

Gene Name

CASP8

Full Name

Caspase-8

Weight

55.391kDa

Superfamily

peptidase C14A family

Alternative Names

AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CASP8, check out the CASP8 Infographic

CASP8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CASP8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1735 has been cited in 16 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (%u2212)-gossypol

Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL

Inhibitory activity of apogossypol in human prostate cancerin vitroandin vivo

Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir

Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (?)-gossypol

Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells

2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL

Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.

Lin Hd, He Cq, Luo Ql, Zhang Jl, Zeng Dx. Rheumatol Int. 2012 Mar;32(3):759-66. Doi: 10.1007/S00296-010-1629-5. Epub 2010 Dec 28. The Effect Of Low-Level Laser To Apoptosis Of Chondrocyte And Caspases Expression, Including Caspase-8 And Caspase-3 ...

Guo H, Luo Q, Zhang J, Lin H, Xia L, He C. Joint Bone Spine. 2011 Dec;78(6):604-10. Doi: 10.1016/J.Jbspin.2011.01.009. Epub 2011 Mar 11. Comparing Different Physical Factors On Serum Tnf-?? Levels, Chondrocyte Apoptosis, Caspase-3 And Caspase-8 Ex...

Zeng D, Luo Q, Lin H, Zhang J, He C. Rheumatol Int. 2012 Dec;32(12):3771-7. Doi: 10.1007/S00296-011-2196-0. Epub 2011 Dec 14. The Effect Of Therapeutic Ultrasound To Apoptosis Of Chondrocyte And Caspase-3 And Caspase-8 Expression In Rabbit Surgery...

Shan S, Li Z, Newton Ip, Zhao C, Li Z, Guo M. Toxicol Lett. 2014 Jun 5;227(2):129-38. Doi: 10.1016/J.Toxlet.2014.03.008. Epub 2014 Mar 28. A Novel Protein Extracted From Foxtail Millet Bran Displays Anti-Carcinogenic Effects In Human Colon Cancer ...

Zhan W, Hu X, Yi J, An Q, Huang X. Mol Med Rep. 2015 Jun;11(6):4142-8. Doi: 10.3892/Mmr.2015.3326. Epub 2015 Feb 10. Inhibitory Activity Of Apogossypol In Human Prostate Cancer In Vitro And In Vivo.

Yan Y, Liang B, Zhang J, Liu Y, Bu X. Mol Med Rep. 2015 Jan;11(1):317-26. Doi: 10.3892/Mmr.2014.2657. Epub 2014 Oct 15. Apoptotic Induction Of Lung Adenocarcinoma A549 Cells Infected By Recombinant Rvg Newcastle Disease Virus (Rl-Rvg) In Vitro.

Xia L, Luo Ql, Lin Hd, Zhang Jl, Guo H, He Cq. Rheumatol Int. 2012 Sep;32(9):2847-56. Doi: 10.1007/S00296-011-2080-Y. Epub 2011 Sep 1. The Effect Of Different Treatment Time Of Millimeter Wave On Chondrocyte Apoptosis, Caspase-3, Caspase-8, And Mm...

Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.

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17 Customer Q&As for Anti-Caspase-8(p18)/CASP8 Antibody

Question

Is this PA1735 anti-Caspase-8(p18)/CASP8 antibody reactive to the isotypes of CASP8?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

The immunogen of PA1735 anti-Caspase-8(p18)/CASP8 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-8 (p18) (257-277aa NNHNFAKAREKVPKLHSIRDR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-17

Question

We want using your anti-Caspase-8(p18)/CASP8 antibody for activation of cysteine-type endopeptidase activity involved in apoptotic process studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

We appreciate your inquiry. This PA1735 anti-Caspase-8(p18)/CASP8 antibody is validated on a549 whole cell lysate, hela whole cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-29

Question

Is a blocking peptide available for product anti-Caspase-8(p18)/CASP8 antibody (PA1735)?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

We do provide the blocking peptide for product anti-Caspase-8(p18)/CASP8 antibody (PA1735). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-15

Question

Would anti-Caspase-8(p18)/CASP8 antibody PA1735 work for IHC with t-cell?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

According to the expression profile of t-cell, CASP8 is highly expressed in t-cell. So, it is likely that anti-Caspase-8(p18)/CASP8 antibody PA1735 will work for IHC with t-cell.

Boster Scientific Support

Answered: 2019-07-17

Question

Will PA1735 anti-Caspase-8(p18)/CASP8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

You can see on the product datasheet, PA1735 anti-Caspase-8(p18)/CASP8 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-01

Question

I got a high background from the bands on my western blot gel. Here are the parameters that I used: - Protein loading 70 µg - SDS gel 10% - Electrophoresis 60V 60 min & 120V 95 min; - Blocking 5% milk RT 30 min - Primary antibody 1:2000 dilution & 4°C overnight incubation - Secondary antibody α-Rabbit –HRP RT incubation for 1 hour Please advise how we can lower the background.

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

Try to repeat your western blot with the following changes: - Protein loading 50 µg - Blocking 5% non-fat dry milk & incubate at 4°C for 2 hours with slow shaking - Primary antibody 1:4000 dilution Then the background of your gel should be reduced.

Boster Scientific Support

Answered: 2019-05-31

Question

We are currently using anti-Caspase-8(p18)/CASP8 antibody PA1735 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

The anti-Caspase-8(p18)/CASP8 antibody (PA1735) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-24

Question

We are interested in to test anti-Caspase-8(p18)/CASP8 antibody PA1735 on human t-cell for research purposes, then I may be interested in using anti-Caspase-8(p18)/CASP8 antibody PA1735 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

L. Carter

Verified customer

Asked: 2019-03-14

Answer

The products we sell, including anti-Caspase-8(p18)/CASP8 antibody PA1735, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-03-14

Question

I see that the anti-Caspase-8(p18)/CASP8 antibody PA1735 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-12-27

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-12-27

Question

My question regarding product PA1735, anti-Caspase-8(p18)/CASP8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Lewis

Verified customer

Asked: 2018-11-21

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1735 anti-Caspase-8(p18)/CASP8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-11-21

Question

We ordered your anti-Caspase-8(p18)/CASP8 antibody for IHC on leukocyte a few months ago. I am using human, and We are going to use the antibody for WB next. We need examining leukocyte as well as small intestine peyer's patch in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

I viewed the website and datasheets of our anti-Caspase-8(p18)/CASP8 antibody and I see that PA1735 has been validated on human in both IHC and WB. Thus PA1735 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-07-11

Question

Our team were content with the WB result of your anti-Caspase-8(p18)/CASP8 antibody. However we have been able to see positive staining in b-cell thymus cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP8 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-05-30

Answer

From what I have seen in literature, b-cell thymus does express CASP8. Generally CASP8 expresses in cytoplasm. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496

Boster Scientific Support

Answered: 2018-05-30

Question

Please see the WB image, lot number and protocol we used for t-cell using anti-Caspase-8(p18)/CASP8 antibody PA1735. Please let me know if you require anything else.

T. Huang

Verified customer

Asked: 2018-03-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-03-20

Question

I was wanting to use your anti-Caspase-8(p18)/CASP8 antibody for IHC for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?

Verified Customer

Verified customer

Asked: 2018-01-11

Answer

You can see on the product datasheet, PA1735 anti-Caspase-8(p18)/CASP8 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-11

Question

Is there a BSA free version of anti-Caspase-8(p18)/CASP8 antibody PA1735 available?

A. Wu

Verified customer

Asked: 2017-11-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-8(p18)/CASP8 antibody PA1735 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-11-17

Question

We have observed staining in human leukocyte. What should we do? Is anti-Caspase-8(p18)/CASP8 antibody supposed to stain leukocyte positively?

D. Jackson

Verified customer

Asked: 2014-12-25

Answer

Based on literature leukocyte does express CASP8. Based on Uniprot.org, CASP8 is expressed in small intestine peyer's patch, b-cell thymus, t-cell, leukocyte, among other tissues. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496

Boster Scientific Support

Answered: 2014-12-25

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Caspase-8(p18)/CASP8 antibody PA1735. Let me know if you need anything else.

J. Krishna

Verified customer

Asked: 2013-12-11

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-12-11

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PA1735
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