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Product Info Summary
| SKU: | PB9331 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Caspase-8/CASP8 Antibody Picoband®
SKU/Catalog Number
PB9331
PB0284 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-8/CASP8 Antibody Picoband® catalog # PB9331. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-8/CASP8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9331)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Caspase 8 recombinant protein (Position: S388-P480). Mouse Caspase 8 shares 83% amino acid (aa) sequence identity with human Caspase 8.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9331 is reactive to Casp8 in Mouse
Observed Molecular Weight
55 kDa
Calculated molecular weight
55.4 kDa
Background of Casp8
CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9331 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Spleen Tissue, Mouse Thymus Tissue, Mouse Kidney Tissue, Mouse Lung Tissue, HEPA Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Caspase 8 Picoband antibody, PB9331, Western blotting
All lanes: Anti Caspase 8 (PB9331) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Thymus Tissue Lysate at 50ug
Lane 3: Mouse Kidney Tissue Lysate at 50ug
Lane 4: Mouse Lung Tissue Lysate at 50ug
Lane 5: HEPA Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
Specific Publications For Anti-Caspase-8/CASP8 Antibody Picoband® (PB9331)
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Customer Q&As
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15 Customer Q&As for Anti-Caspase-8/CASP8 Antibody Picoband®
Question
Does PB9331 anti-Caspase-8/CASP8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
It shows on the product datasheet, PB9331 anti-Caspase-8/CASP8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-01
Question
Will anti-Caspase-8/CASP8 antibody PB9331 work for WB with b-cell thymus?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
According to the expression profile of b-cell thymus, CASP8 is highly expressed in b-cell thymus. So, it is likely that anti-Caspase-8/CASP8 antibody PB9331 will work for WB with b-cell thymus.
Boster Scientific Support
Answered: 2019-11-25
Question
I see that the anti-Caspase-8/CASP8 antibody PB9331 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell thymus using anti-Caspase-8/CASP8 antibody PB9331. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-04
Question
I am interested in using your anti-Caspase-8/CASP8 antibody for positive regulation of neuron death studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
We appreciate your inquiry. This PB9331 anti-Caspase-8/CASP8 antibody is tested on mouse thymus tissue, spleen tissue, tissue lysate, kidney tissue, lung tissue, hepa cell lysate. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-31
Question
Our lab want to know about to test anti-Caspase-8/CASP8 antibody PB9331 on mouse b-cell thymus for research purposes, then I may be interested in using anti-Caspase-8/CASP8 antibody PB9331 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The products we sell, including anti-Caspase-8/CASP8 antibody PB9331, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-29
Question
Is this PB9331 anti-Caspase-8/CASP8 antibody reactive to the isotypes of CASP8?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
The immunogen of PB9331 anti-Caspase-8/CASP8 antibody is E.coli-derived mouse Caspase 8 recombinant protein (Position: S388-P480). Mouse Caspase 8 shares 83% amino acid (aa) sequence identity with human Caspase 8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-30
Question
We are currently using anti-Caspase-8/CASP8 antibody PB9331 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
The anti-Caspase-8/CASP8 antibody (PB9331) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-17
Question
Please see the WB image, lot number and protocol we used for b-cell thymus using anti-Caspase-8/CASP8 antibody PB9331. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-25
Question
My boss were well pleased with the WB result of your anti-Caspase-8/CASP8 antibody. However we have been able to see positive staining in b-cell thymus cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP8 supposed to be expressed?
A. Jones
Verified customer
Asked: 2018-04-24
Answer
According to literature, b-cell thymus does express CASP8. Generally CASP8 expresses in cytoplasm. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2018-04-24
Question
We have seen staining in mouse leukocyte. What should we do? Is anti-Caspase-8/CASP8 antibody supposed to stain leukocyte positively?
G. Yang
Verified customer
Asked: 2018-01-19
Answer
From literature leukocyte does express CASP8. From Uniprot.org, CASP8 is expressed in small intestine peyer's patch, b-cell thymus, t-cell, leukocyte, among other tissues. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2018-01-19
Question
Do you have a BSA free version of anti-Caspase-8/CASP8 antibody PB9331 available?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-8/CASP8 antibody PB9331 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-01
Question
I was wanting to use your anti-Caspase-8/CASP8 antibody for WB for mouse b-cell thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse b-cell thymus identification?
M. Taylor
Verified customer
Asked: 2016-07-12
Answer
As indicated on the product datasheet, PB9331 anti-Caspase-8/CASP8 antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse b-cell thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-07-12
Question
Is a blocking peptide available for product anti-Caspase-8/CASP8 antibody (PB9331)?
R. Edwards
Verified customer
Asked: 2014-12-08
Answer
We do provide the blocking peptide for product anti-Caspase-8/CASP8 antibody (PB9331). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-12-08
Question
I have a question about product PB9331, anti-Caspase-8/CASP8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Carter
Verified customer
Asked: 2014-02-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9331 anti-Caspase-8/CASP8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-02-24
