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Anti-Caspase-8/CASP8 Antibody Picoband™
Boster Bio Anti-Caspase-8/CASP8 Antibody Picoband™ catalog # PB9331. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-8/CASP8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9331)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived mouse Caspase 8 recombinant protein (Position: S388-P480). Mouse Caspase 8 shares 83% amino acid (aa) sequence identity with human Caspase 8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
PB9331 is reactive to Casp8 in Mouse
PB9331 is guaranteed for WB Boster Guarantee
Background of Caspase-8
CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Anti-Caspase 8 Picoband antibody, PB9331, Western blotting
All lanes: Anti Caspase 8 (PB9331) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Thymus Tissue Lysate at 50ug
Lane 3: Mouse Kidney Tissue Lysate at 50ug
Lane 4: Mouse Lung Tissue Lysate at 50ug
Lane 5: HEPA Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
Gene/Protein Information For Casp8 (Source: Uniprot.org, NCBI)
peptidase C14A family
AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy Casp8|CASP-8, Casp, FLI, FLICE, MAC, MACH, Mch5|caspase 8|caspase-8|Fas-linked ICE-like protease*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Casp8, check out the Casp8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Casp8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
PB9331 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (%u2212)-gossypol
Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL
Inhibitory activity of apogossypol in human prostate cancerin vitroandin vivo
Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (?)-gossypol
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL
Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.
Lin Hd, He Cq, Luo Ql, Zhang Jl, Zeng Dx. Rheumatol Int. 2012 Mar;32(3):759-66. Doi: 10.1007/S00296-010-1629-5. Epub 2010 Dec 28. The Effect Of Low-Level Laser To Apoptosis Of Chondrocyte And Caspases Expression, Including Caspase-8 And Caspase-3 ...
Guo H, Luo Q, Zhang J, Lin H, Xia L, He C. Joint Bone Spine. 2011 Dec;78(6):604-10. Doi: 10.1016/J.Jbspin.2011.01.009. Epub 2011 Mar 11. Comparing Different Physical Factors On Serum Tnf-?? Levels, Chondrocyte Apoptosis, Caspase-3 And Caspase-8 Ex...
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