Product Info Summary
SKU: | M00080 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00080
BM4619 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody catalog # M00080. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00080)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GEO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Caspase-9
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00080 is reactive to CASP9 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
46 kDa
Calculated molecular weight
46281 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00080 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human K562 whole cell, human SiHa whole cell, human HEL whole cell, human Jurkat whole cell, human Jurkat whole cell, mouse NIH/3T3 whole cell, mouse NIH/3T3 whole cell
IHC: human liver tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Caspase-9 using anti-Caspase-9 antibody (M00080).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-9 antigen affinity purified monoclonal antibody (Catalog # M00080) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-9 at approximately 46 kDa. The expected band size for Caspase-9 is at 46 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human liver, using Caspase-9 Antibody.
Click image to see more details
Immunofluorescent analysis of HeLa cells treated with staurosporine, using Caspase-9 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CASP9 (Source: Uniprot.org, NCBI)
Gene Name
CASP9
Full Name
Caspase-9
Weight
46281 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-9;CASP-9;3.4.22.62;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6;Caspase-9 subunit p35;Caspase-9 subunit p10;CASP9;MCH6; CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 caspase 9 caspase-9|ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9, apoptosis-related cysteine peptidase|protein phosphatase 1, regulatory subunit 56
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP9, check out the CASP9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody (M00080)
Hello CJ!
M00080 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line
In vivo?and?in vitro?evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer
Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.
Dai C, Li J, Tang S, Li J, Xiao X. Antimicrob Agents Chemother. 2014 Jul;58(7):4075-85. Doi: 10.1128/Aac.00070-14. Epub 2014 May 5. Colistin-Induced Nephrotoxicity In Mice Involves The Mitochondrial, Death Receptor, And Endoplasmic Reticulum Pathw...
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody
Question
We have observed staining in mouse cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-Caspase-9 Rabbit Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
From literature cervix carcinoma erythroleukemia does express CASP9. From Uniprot.org, CASP9 is expressed in adrenal tissue, t-cell, stomach cancer, kidney, eye lymph, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP9 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, and Lymph, Pubmed ID: 15489334
Kidney, Pubmed ID: 14702039
Stomach cancer, Pubmed ID: 9890966
T-cell, Pubmed ID: 8900201
Boster Scientific Support
Answered: 2020-01-20
Question
I was wanting to use your anti-Caspase-9 Rabbit Monoclonal antibody for WB for human adrenal tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adrenal tissue identification?
S. Bhatt
Verified customer
Asked: 2020-01-17
Answer
You can see on the product datasheet, M00080 anti-Caspase-9 Rabbit Monoclonal antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human adrenal tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-17
Question
We are currently using anti-Caspase-9 Rabbit Monoclonal antibody M00080 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
The anti-Caspase-9 Rabbit Monoclonal antibody (M00080) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-15
Question
I am interested in to test anti-Caspase-9 Rabbit Monoclonal antibody M00080 on human adrenal tissue for research purposes, then I may be interested in using anti-Caspase-9 Rabbit Monoclonal antibody M00080 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
The products we sell, including anti-Caspase-9 Rabbit Monoclonal antibody M00080, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-28
Question
Would anti-Caspase-9 Rabbit Monoclonal antibody M00080 work for WB with adrenal tissue?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
According to the expression profile of adrenal tissue, CASP9 is highly expressed in adrenal tissue. So, it is likely that anti-Caspase-9 Rabbit Monoclonal antibody M00080 will work for WB with adrenal tissue.
Boster Scientific Support
Answered: 2019-10-10
Question
Is there a BSA free version of anti-Caspase-9 Rabbit Monoclonal antibody M00080 available?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-9 Rabbit Monoclonal antibody M00080 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal tissue using anti-Caspase-9 Rabbit Monoclonal antibody M00080. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-26
Question
My question regarding product M00080, anti-Caspase-9 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00080 anti-Caspase-9 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-27
Question
I see that the anti-Caspase-9 Rabbit Monoclonal antibody M00080 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-17
Question
Is a blocking peptide available for product anti-Caspase-9 Rabbit Monoclonal antibody (M00080)?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
We do provide the blocking peptide for product anti-Caspase-9 Rabbit Monoclonal antibody (M00080). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-13
Question
See attached the WB image, lot number and protocol we used for adrenal tissue using anti-Caspase-9 Rabbit Monoclonal antibody M00080. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-28
Question
Our lab want to know about using your anti-Caspase-9 Rabbit Monoclonal antibody for cellular response to dexamethasone stimulus studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
K. Dhar
Verified customer
Asked: 2015-01-22
Answer
I appreciate your inquiry. This M00080 anti-Caspase-9 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-01-22
Question
Is this M00080 anti-Caspase-9 Rabbit Monoclonal antibody reactive to the isotypes of CASP9?
A. Krishna
Verified customer
Asked: 2014-08-18
Answer
The immunogen of M00080 anti-Caspase-9 Rabbit Monoclonal antibody is A synthesized peptide derived from human Caspase-9 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-08-18
Question
Will M00080 anti-Caspase-9 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Anderson
Verified customer
Asked: 2014-06-26
Answer
It shows on the product datasheet, M00080 anti-Caspase-9 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-06-26