Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11)
|Ig Isotype||Mouse IgG1|
|Applications||IHC, ICC, WB|
|Product Name||Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Catenin gamma (Plakoglobin), junction plakoglobin ( JUP) detection. Tested with WB, IHC-F, ICC in Human;bovine. No cross reactivity with other proteins.|
|Cite This Product||Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1012)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Recombinant chicken plakoglobin.|
Assay Dilutions Overview
Immunocytochemistry , 1μg/ml, Human, bovine, -
Immunohistochemistry(Frozen Section), 4μg/ml, Human, bovine, -
Western blot, 2μg/ml, Human, bovine
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
Images And Assay Conditions
Anti-Catenin gamma(Plakoglobin) antibody (monoclonal), MA1012, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: HT1080 Cell Lysate
Protein Target Info (Source: Uniprot.org)
|Protein Name||Junction plakoglobin|
|Alternative Names||Junction plakoglobin;Jup;|
|Subcellular Localization||Cell junction, adherens junction . Cell junction, desmosome . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Cytoplasmic in a soluble and membrane-associated form. .|
|Molecular Weight||81801 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). .|
|Research Areas||Bovine, Human
*You can search these to find other products in these research areas.
|Background||Junction Plakoglobin(JUP) ,also knows as catenin gamma, is a major cytoplasmic protein that occurs in a soluble and a membrane-associated form and is the only known constituent common to the submembranous plaques of both kinds of adhering junctions, the desmosomes and the intermediate junctions. It is a component of the cadherin-catenin complex, which is predominantly localized where actin filaments anchor in adherens junctions of epithelial cells. The human plakoglobin gene localizes on chromosome 17q21. Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact firstname.lastname@example.org
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to email@example.com.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to gamma catenin antibody, plakoglobin antibody