Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11)

SKU MA1012
Size 100μg/vial
Reactivity Bovine, Human
Clonality Monoclonal
Clone Number 15F11
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, ICC, WB

Overview

Product Name Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11)
SKU/Catalog Number MA1012
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Catenin gamma (Plakoglobin), junction plakoglobin ( JUP) detection. Tested with WB, IHC-F, ICC in Human;bovine. No cross reactivity with other proteins.
Cite This Product Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1012)
Host Mouse
Immunogen Recombinant chicken plakoglobin.
Reactivity Bovine, Human

Assay Details

Assay Dilutions Overview

Immunocytochemistry , 1μg/ml, Human, bovine, -
Immunohistochemistry(Frozen Section), 4μg/ml, Human, bovine, -
Western blot, 2μg/ml, Human, bovine

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Images And Assay Conditions

/antibody/ma1012 1 WB anti catenin gamma plakoglobin antibody monoclonal 15f11.jpg

Anti-Catenin gamma(Plakoglobin) antibody (monoclonal), MA1012, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: HT1080 Cell Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q6P0K8
Gene Name Jup
Protein Name Junction plakoglobin
Alternative Names Junction plakoglobin;Jup;
Subcellular Localization Cell junction, adherens junction . Cell junction, desmosome . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Cytoplasmic in a soluble and membrane-associated form. .
Molecular Weight 81801 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). .
Background Junction Plakoglobin(JUP) ,also knows as catenin gamma, is a major cytoplasmic protein that occurs in a soluble and a membrane-associated form and is the only known constituent common to the submembranous plaques of both kinds of adhering junctions, the desmosomes and the intermediate junctions. It is a component of the cadherin-catenin complex, which is predominantly localized where actin filaments anchor in adherens junctions of epithelial cells. The human plakoglobin gene localizes on chromosome 17q21. Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin.

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Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to gamma catenin antibody, plakoglobin antibody