|Validated Species:||Bovine, Human|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11)|
|Description||Mouse IgG monoclonal antibody for Catenin gamma (Plakoglobin), junction plakoglobin ( JUP) detection. Tested with WB, IHC-F, ICC in Human;bovine. No cross reactivity with other proteins.|
|Cite This Product||Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11) 15F11 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1012)|
|Replacement Item||This antibody may replace the following items: sc-83058|sc-98673|sc-271002 from Santa Cruz Biotechnology.|
|Validated Species||Bovine, Human|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant chicken plakoglobin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Junction plakoglobin|
|Molecular Weight||81801 MW|
|Protein Function||Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). .|
|Sequence Similarities||Belongs to the beta-catenin family.|
|Subcellular Localization||Cell junction, adherens junction . Cell junction, desmosome . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Cytoplasmic in a soluble and membrane-associated form. .|
|Alternative Names||Junction plakoglobin;Jup;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|catenins||
Background for Junction plakoglobin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 1μg/ml, Human, bovine, -|
Immunohistochemistry(Frozen Section), 4μg/ml, Human, bovine, -
Western blot, 2μg/ml, Human, bovine
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11) Images
Click the images to enlarge.
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