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Product Info Summary
|Reactive Species:||Bovine, Human|
|Application:||IHC, ICC, WB|
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Anti-Catenin Gamma (Plakoglobin) Jup Antibody (Monoclonal, 15F11)
Boster Bio Anti-Catenin Gamma (Plakoglobin) Jup Antibody (Monoclonal, 15F11) catalog # MA1012. Tested in IHC, ICC, WB applications. This antibody reacts with Bovine, Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Catenin Gamma (Plakoglobin) Jup Antibody (Monoclonal, 15F11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1012)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Recombinant chicken plakoglobin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
MA1012 is reactive to Jup in Bovine, Human
MA1012 is guaranteed for IHC, ICC, WB Boster Guarantee
Background of gamma Catenin
Junction Plakoglobin (JUP) ,also knows as catenin gamma, is a major cytoplasmic protein that occurs in a soluble and a membrane-associated form and is the only known constituent common to the submembranous plaques of both kinds of adhering junctions, the desmosomes and the intermediate junctions. It is a component of the cadherin-catenin complex, which is predominantly localized where actin filaments anchor in adherens junctions of epithelial cells. The human plakoglobin gene localizes on chromosome 17q21. Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, bovine, -
Immunohistochemistry (Frozen Section), 4μg/ml, Human, bovine, -
Western blot, 1μg/ml, Human, bovine
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Catenin gamma(Plakoglobin) using anti-Catenin gamma(Plakoglobin) antibody (MA1012).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human CACO-2 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human 293T whole cell lysates,
Lane 8: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Catenin gamma(Plakoglobin) antigen affinity purified monoclonal antibody (Catalog # MA1012) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Catenin gamma(Plakoglobin) at approximately 82 kDa. The expected band size for Catenin gamma(Plakoglobin) is at 82 kDa.
Gene/Protein Information For Jup (Source: Uniprot.org, NCBI)
catenin (cadherin-associated protein), gamma (80kD); catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; Desmoplakin III; desmoplakin-3; DP3CTNNG; DPIII; gamma-catenin; junction plakoglobin; PDGBARVD12; PKGB Jup|junction plakoglobin|junction plakoglobin|gamma-catenin (plakoglobin)*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Jup, check out the Jup Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Jup: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
Specific Publications For Anti-Catenin Gamma (Plakoglobin) Jup Antibody (Monoclonal, 15F11) (MA1012)
No publications found for MA1012
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