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- Table of Contents
CTSB antibody (A01456-3) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of SiHa cells using anti-Cathepsin B/CTSB antibody (A01456-3).
Overlay histogram showing SiHa cells stained with A01456-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cathepsin B/CTSB Antibody (A01456-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with A01456-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cathepsin B/CTSB Antibody (A01456-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cathepsin B/CTSB Antibody Picoband®
CTSB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
CTSB antibody was positively detected by WB in:
human U87 whole cell, human HepG2 whole cell, human A549 whole cell, rat brain tissue, rat liver tissue, mosue liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CTSB antibody was positively detected by IHC in:
human liver cancer tissue, human rectal cancer tissue, human thyroid cancer tissue, mouse liver tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CTSB antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Cathepsin B/CTSB Antibody Picoband®
View all CTSB Antibodies
View all CTSB Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cathepsin B/CTSB recombinant protein (Position: Q42-K339).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
23 kDa, 31 kDa
Calculated Molecular Weight
37.8 kDa
Background of CTSB
Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. And this gene is mapped to chromosome 8p22. The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.
Cite This Product
Anti-Cathepsin B/CTSB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01456-3)
Protocols
Western Blot protocol for CTSB antibody
Immunohistochemistry (IHC) protocol for CTSB antibody
Flow Cytometry (FC) protocol for CTSB antibody
Publications
Application:
Species:
Erythrabyssin II is identified as a novel late-stage autophagy inhibitor that reverses chemoresistance and promotes apoptosis in ovarian cancer
Published: 2025-06-11
Journal: iScience
Application: WB
Impact Factor: 4.10
Species: Human
Discovery of Highly Potent and Orally Bioavailable Histone Deacetylase 3 Inhibitors as Immunomodulators and Enhancers of DNA-Damage Response in Cancer Therapy
Published: 2025-01-28
Journal: JOURNAL OF MEDICINAL CHEMISTRY
Application: WB
Impact Factor: 6.80
Species: Mouse
PMID: 39873221
Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis
Published: 2024-02-26
Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
Application: WB
Impact Factor: 1.50
Species: Chicken
PMID: 38427138
The marine natural product, dicitrinone B, induces apoptosis through autophagy blockade in breast cancer
Published: 2022-09-02
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 5.31
Species: Human
PMID: 36052845
CTSB promotes porcine preadipocytes differentiation by degrading fibronectin and attenuating the Wnt/β-catenin signaling pathway
Published: 2014-05-31
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: IF,WB
Impact Factor: 2.39
Species: Pig
PMID: 24878992
The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function
Published: 2014-05-10
Journal: BIOMATERIALS
Application: WB
Impact Factor: 8.31
Species: Human
PMID: 24818882
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Product Promise
We promise Anti-Cathepsin B/CTSB Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01456-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026