Anti-CaV1.3/CACNA1D Antibody Picoband™

Product Name

Anti-CaV1.3/CACNA1D Antibody Picoband™

View all Cav1.3 Antibodies

SKU/Catalog Number

PB9329

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CaV1.3/CACNA1D Antibody Picoband™ catalog # PB9329. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CaV1.3/CACNA1D Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9329)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CaV1.3 recombinant protein (Position: M1-I180). Human CaV1.3 shares 99% and 98%amino acid (aa) sequence identity with mouse and rat CaV1.3, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9329 is reactive to CACNA1D in Human, Mouse, Rat

Applications

PB9329 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

245 kDa

Calculated molecular weight

245.141kDa

Background of Cav1.3

CACNA1D is also known as PASNA, SANDD or Cav1.3. Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CAV1.3 using anti-CAV1.3 antibody (PB9329).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate,
Lane 2: Mouse Brain Tissue Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV1.3 antigen affinity purified polyclonal antibody (Catalog # PB9329) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAV1.3 at approximately 245KD. The expected band size for CAV1.3 is at 245KD.

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Figure 2. Flow Cytometry analysis of U-87 cells using anti-CaV1.3 antibody (PB9329).
Overlay histogram showing U-87 cells stained with PB9329 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaV1.3 Antibody (PB9329,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CACNA1D (Source: Uniprot.org, NCBI)

Alternative Names

CACN4calcium channel, neuroendocrine/brain-type, alpha 1 subunit; CACNL1A2voltage-dependent L-type calcium channel subunit alpha-1D; Calcium channel, L type, alpha-1 polypeptide, isoform 2; calcium channel, voltage-dependent, L type, alpha 1D subunit; Cav1.3; CCHL1A2voltage-gated calcium channel alpha 1 subunit; L type, alpha-1 polypeptide; voltage-gated calcium channel alpha subunit Cav1.3; Voltage-gated calcium channel subunit alpha Cav1.3 CACNA1D CACH3, CACN4, CACNL1A2, CCHL1A2, Cav1.3, PASNA, SANDD calcium voltage-gated channel subunit alpha1 D voltage-dependent L-type calcium channel subunit alpha-1D|calcium channel, L type, alpha-1 polypeptide|calcium channel, neuroendocrine/brain-type, alpha 1 subunit|calcium channel, voltage-dependent, L type, alpha 1D subunit|voltage-gated calcium channel alpha 1 subunit|voltage-gated calcium channel alpha subunit Cav1.3

For more info on CACNA1D, check out the CACNA1D Infographic

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In this infographic, you will see the following information for CACNA1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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