Anti-CCDC134 Antibody

Product Name

Anti-CCDC134 Antibody

View all CCDC134 Antibodies

SKU/Catalog Number

A14619

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CCDC134 Antibody (Catalog # A14619). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CCDC134 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14619)

Host

Rabbit

Contents

CCDC134 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CCDC134 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CCDC134. The immunogen is located within the first 50 amino acids of CCDC134.

Reactive Species

A14619 is reactive to CCDC134 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

26.6 kDa

Background of CCDC134

The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. One such protein is CCDC134, a recently identified secretory protein that has been found to inhibit the transcriptional activity of the Elk1 protein. Overexpression CCDC134 also inhibited the phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while specific siRNA against CCDC134 activated Elk1 transcriptional activity and the phosphorylation of Erk and JNK/SAPK, suggesting a potential inhibiting role of CCDC134 in MAPK-mediated Elk1 transcription. CCDC134 is widely expressing in normal adult tissues, tumors, and cell lines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14619 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CCDC134 antibody can be used for detection of CCDC134 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CCDC134

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