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Product Info Summary
| SKU: | A08439 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-CCDC22 Antibody
SKU/Catalog Number
A08439
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CCDC22 Antibody (Catalog # A08439). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CCDC22 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CCDC22 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08439)
Host
Rabbit
Contents
CCDC22 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CCDC22 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CCDC22. The immunogen is located within amino acids 530 - 580 of CCDC22.
Cross-reactivity
At least three isoforms of CCDC22 are known to exist; this antibody will detect the two largest isoforms.
Reactive Species
A08439 is reactive to CCDC22 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
70.8 kDa
Background of CCDC22
CCDC22 is a recently identified coiled-coil domain-containing protein that has been shown to bind copines, which are calcium-dependent, membrane-binding proteins that may function in calcium signaling. In rat, CCDC22 has been observed to localize in multiple regions of the brain, including the prefrontal and somatosensory cortex, dentate gyrus and thalamus, and in the ipsilateral motor neurons of the spinal cord after sciatic nerve transection suggesting that it may play a role in neuronal injury response. The human CCDC22 gene has been identified as a novel candidate gene for syndromic X-linked intellectual disability (XLID).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08439 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CCDC22 antibody can be used for detection of CCDC22 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CCDC22 in 293 cell lysate with CCDC22 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of CCDC22 in human brain tissue with CCDC22 antibody at 20 μg/mL.
Specific Publications For Anti-CCDC22 Antibody (A08439)
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