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Product Info Summary
| SKU: | A10218 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Chicken |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-CCDC55 NSRP1 Antibody
SKU/Catalog Number
A10218
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CCDC55 NSRP1 Antibody (Catalog # A10218). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CCDC55 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CCDC55 NSRP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10218)
Host
Chicken
Contents
CCDC55 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgY
Immunogen
CCDC55 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human CCDC55. The immunogen is located within amino acids 430 - 480 of CCDC55.
Cross-reactivity
At least three isoforms are known to exist; this antibody will detect all three.
Reactive Species
A10218 is reactive to NSRP1 in Human, Mouse, Rat
Calculated molecular weight
66.4 kDa
Background of NSRP1
CCDC55, also known as Nuclear speckle-related protein 70 (NSrp70), was initially identified by the NIH Mammalian Gene Collection. Nuclear speckles are known to be the storage sites of mRNA splicing regulators. CCDC55 has been suggested to modulate alternative pre-mRNA splicing in vivo. It co-localizes and physically interacts with the SR proteins SC35 and ASF/SF2, essential splicing factors which are required for constitutive splicing and can regulate alternative mRNA splicing. Loss of CCDC55 in mice leads to early embryonic lethality highlights the importance of the functional role of CCDC55.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10218 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CCDC55 antibody can be used for detection of CCDC55 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CCDC55 in human brain tissue lysate with CCDC55 antibody at (A) 0.5 and (B) 1 μg/mL
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Immunofluorescence of CCDC55 in mouse brain cells with CCDC55 antibody at 20 μg/mL.
Specific Publications For Anti-CCDC55 NSRP1 Antibody (A10218)
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