Anti-Ccl21a Antibody Picoband™

Product Name

Anti-Ccl21a Antibody Picoband™

View all CCL21/6Ckine Antibodies

SKU/Catalog Number

A33349

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ccl21a Antibody Picoband™ catalog # A33349. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Ccl21a Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33349)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Ccl21a recombinant protein (Position: S24-G133).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A33349 is reactive to Ccl21a in Mouse, Rat

Applications

A33349 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

14 kDa

Calculated molecular weight

14377 MW

Background of CCL21/6Ckine

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. IHC analysis of Ccl21a using anti-Ccl21a antibody (A33349).
Ccl21a was detected in a paraffin-embedded section of mouse lung tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ccl21a Antibody (A33349) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Click image to see more details

Figure 2. IHC analysis of Ccl21a using anti-Ccl21a antibody (A33349).
Ccl21a was detected in a paraffin-embedded section of rat lung tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ccl21a Antibody (A33349) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For Ccl21a (Source: Uniprot.org, NCBI)

Alternative Names

6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4 Ccl21a|6CKBAC, 6CKBAC2, 6Cki, 6Ckine, ALP, AW987545, CKb9, Exodus, Gm1987, S, SCYA21a, SLC, Scya, Scya2, Scya21, Scya21b, Tca, Tca4, plt|chemokine (C-C motif) ligand 21A (serine)|C-C motif chemokine 21a|CC chemokine 6Ckine-ser|CCL21-Ser|beta-chemokine exodus-2|exodus-2|small inducible cytokine A21a|thymus-derived chemotactic agent 4

For more info on Ccl21a, check out the Ccl21a Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ccl21a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Ccl21a Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis