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Product Info Summary
| SKU: | AZQ6NVI6 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish CCT5 Antibody Picoband®
SKU/Catalog Number
AZQ6NVI6
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish CCT5 Antibody Picoband® catalog # AZQ6NVI6. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20 ℃ for one year from date of receipt. After reconstitution, at 4 ℃ for one month. It can also be aliquotted and stored frozen at -20 ℃ for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish CCT5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6NVI6)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish CCT5 recombinant protein (Position: S442-D541).
Reactive Species
AZQ6NVI6 is reactive to CCT5 in Zebrafish
Observed Molecular Weight
59 kDa
Background of CCT5
CCT5 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ6NVI6 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Positive Control
WB: zebrafish head tissue, zebrafish embryo tissue
Validation Images & Assay Conditions
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Western blot analysis of Zebrafish CCT5 using anti-Zebrafish CCT5 antibody (AZQ6NVI6).
Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Zebrafish head tissue lysates,
Lane 2: Zebrafish embyro tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Zebrafish CCT5 antigen affinity purified polyclonal antibody (AZQ6NVI6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Zebrafish CCT5 at approximately 59 kDa. The expected band size for Zebrafish CCT5 is at 59 kDa.
Specific Publications For Anti-Zebrafish CCT5 Antibody Picoband® (AZQ6NVI6)
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