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Product Info Summary
| SKU: | A00483 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD110 MPL Antibody
SKU/Catalog Number
A00483
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD110 MPL Antibody catalog # A00483. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD110 MPL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00483)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Thrombopoietin receptor at AA range: 321-370
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00483 is reactive to MPL in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
71.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00483 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
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Western Blot analysis of MCF7 cells using CD110 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-CD110 MPL Antibody (A00483)
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7 Customer Q&As for Anti-CD110 MPL Antibody
Question
Can you help my question with product A00483, anti-CD110 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00483 anti-CD110 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-19
Question
Here is the WB image, lot number and protocol we used for dorsolateral prefrontal cortex using anti-CD110 antibody A00483. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-05
Question
Will anti-CD110 antibody A00483 work for IHC with dorsolateral prefrontal cortex?
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
According to the expression profile of dorsolateral prefrontal cortex, MPL is highly expressed in dorsolateral prefrontal cortex. So, it is likely that anti-CD110 antibody A00483 will work for IHC with dorsolateral prefrontal cortex.
Boster Scientific Support
Answered: 2018-01-08
Question
I was wanting to use to test anti-CD110 antibody A00483 on human dorsolateral prefrontal cortex for research purposes, then I may be interested in using anti-CD110 antibody A00483 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
The products we sell, including anti-CD110 antibody A00483, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-08
Question
Do you have a BSA free version of anti-CD110 antibody A00483 available?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD110 antibody A00483 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for dorsolateral prefrontal cortex using anti-CD110 antibody A00483. Let me know if you need anything else.
K. Dhar
Verified customer
Asked: 2015-03-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-03-30
Question
We are currently using anti-CD110 antibody A00483 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
M. Roberts
Verified customer
Asked: 2014-06-06
Answer
The anti-CD110 antibody (A00483) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-06
