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- Table of Contents
ITGAM antibody (PB9140) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of THP-1 cells using anti-CD11b antibody (PB9140).
Overlay histogram showing THP-1 cells stained with PB9140 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11b Antibody (PB9140,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with PB9140 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11b Antibody (PB9140,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CD11b/ITGAM Antibody Picoband®
ITGAM antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
ITGAM antibody was positively detected by IHC in:
human tonsil tissue, mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ITGAM antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Recommend
Tested
Recommend
Mouse
Recommend
Tested
Recommend
Rat
Recommend
Tested
Recommend
Product Information
Product Name
Anti-CD11b/ITGAM Antibody Picoband®
View all ITGAM Antibodies
View all ITGAM Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
127.2 kDa
Background of ITGAM
Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.
Cite This Product
Anti-CD11b/ITGAM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9140)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
89%
Moderate Homology
Dog
81%
Moderate Homology
Rabbit
79%
Moderate Homology
Mouse
79%
Moderate Homology
Rat
79%
Moderate Homology
Cow
78%
Moderate Homology
Sheep
76%
Moderate Homology
Zebrafish
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for ITGAM antibody
Flow Cytometry (FC) protocol for ITGAM antibody
Publications
Application:
Species:
Regulatory effects of the long non‑coding RNA RP11‑543N12.1 and microRNA‑324‑3p axis on the neuronal apoptosis induced by the inflammatory reactions of microglia
Published: 2018-06-20
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: IHC
Impact Factor: 2.78
Species: Human
PMID: 29956723
Tanshinone IIA prevents the loss of nigrostriatal dopaminergic neurons by inhibiting NADPH oxidase and iNOS in the MPTP model of Parkinson's disease
Published: 2014-11-27
Journal: JOURNAL OF THE NEUROLOGICAL SCIENCES
Application: WB,IHC
Impact Factor: 2.26
Species: Mouse
PMID: 25491263
Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy
Published: 2014-11-01
Journal: Iranian Journal of Basic Medical Sciences
Application: IHC
Impact Factor: 0.60
Species: Rat
PMID: 25691929
The Neurovascular Protective Effects of Huperzine A on <smlcap>D</smlcap>-Galactose-Induced Inflammatory Damage in the Rat Hippocampus
Published: 2014-08-27
Journal: GERONTOLOGY
Application: WB
Impact Factor: 2.68
Species: Rat
PMID: 24969491
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Published: 2010-07-07
Journal: CELL STRESS & CHAPERONES
Application: IHC
Impact Factor: 2.17
Species: Rat
PMID: 20607472
Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation
Published: 2009-01-22
Journal: CELL BIOLOGY INTERNATIONAL
Application: IHC
Impact Factor: 1.62
Species: Rat
PMID: 19385038
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PB9140
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 24, 2026