Anti-CD11b/ITGAM Antibody Picoband™

ITGAM antibody

Boster Bio Anti-CD11b/ITGAM Antibody Picoband™ catalog # A00144-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: A00144-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

Customers Who Bought This Also Bought

Product Name

Anti-CD11b/ITGAM Antibody Picoband™

View all ITGAM Antibodies

SKU/Catalog Number

A00144-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD11b/ITGAM Antibody Picoband™ catalog # A00144-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD11b/ITGAM Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00144-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human CD11b recombinant protein (Position: N129-E336).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00144-1 is reactive to ITGAM in Human, Mouse, Rat

Applications

A00144-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

170 kDa

Calculated molecular weight

127.179kDa

Background of ITGAM

Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or cluster of differentiation molecule 11B (CD11B). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)

Gene Name

ITGAM

Full Name

Integrin alpha-M

Weight

127.179kDa

Superfamily

integrin alpha chain family

Alternative Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC 1; MAC1; MAC-1; Mac1, alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha polypeptide; Mo1, alpha subunit; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6 ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 antigen-like family member B|CR-3 alpha chain|antigen CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage antigen alpha polypeptide|neutrophil adherence receptor alpha-M subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ITGAM, check out the ITGAM Infographic

ITGAM infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00144-1 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Promoted interaction of C/EBP%u03B1 with demethylated Cxcr3 gene promoter contributes to neuropathic pain in mice

Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats

Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)

Mu L, Jing C, Guo Z. Iran J Basic Med Sci. 2014 Nov;17(11):874-8. Expressions Of Cd11A, Cd11B, And Cd11C Integrin Proteins In Rats With Myocardial Hypertrophy.

Yuan Y, Su Z, Pu Y, Liu X, Chen J, Zhu F, Zhu Y, Zhang H, He C. Br J Pharmacol. 2012 May;166(2):749-63. Doi: 10.1111/J.1476-5381.2011.01804.X. Ethyl Pyruvate Promotes Spinal Cord Repair By Ameliorating The Glial Microenvironment.

Have you used Anti-CD11b/ITGAM Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-CD11b/ITGAM Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

17 Customer Q&As for Anti-CD11b/ITGAM Antibody Picoband™

Question

My question regarding product A00144-1, anti-CD11b/ITGAM antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00144-1 anti-CD11b/ITGAM antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-03

Question

We have observed staining in rat blood. What should we do? Is anti-CD11b/ITGAM antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

Based on literature blood does express ITGAM. Based on Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2020-01-28

Question

My lab would like using your anti-CD11b/ITGAM antibody for negative regulation of dopamine metabolic process studies. Has this antibody been tested with western blotting on mouse thymus tissue? We would like to see some validation images before ordering.

C. Banerjee

Verified customer

Asked: 2020-01-17

Answer

We appreciate your inquiry. This A00144-1 anti-CD11b/ITGAM antibody is validated on rat thymus tissue, spleen tissue, mouse thymus tissue. It is guaranteed to work for ELISA, Flow Cytometry, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-17

Question

Is there a BSA free version of anti-CD11b/ITGAM antibody A00144-1 available?

Verified Customer

Verified customer

Asked: 2019-12-26

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD11b/ITGAM antibody A00144-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-26

Question

I was wanting to use your anti-CD11b/ITGAM antibody for ELISA for rat bone marrow cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat bone marrow cell identification?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

It shows on the product datasheet, A00144-1 anti-CD11b/ITGAM antibody has been validated for ELISA, Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat bone marrow cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-24

Question

I see that the anti-CD11b/ITGAM antibody A00144-1 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-05

Question

Would anti-CD11b/ITGAM antibody A00144-1 work for ELISA with bone marrow cell?

Verified Customer

Verified customer

Asked: 2019-07-31

Answer

According to the expression profile of bone marrow cell, ITGAM is highly expressed in bone marrow cell. So, it is likely that anti-CD11b/ITGAM antibody A00144-1 will work for ELISA with bone marrow cell.

Boster Scientific Support

Answered: 2019-07-31

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow cell using anti-CD11b/ITGAM antibody A00144-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-18

Question

My team were satisfied with the WB result of your anti-CD11b/ITGAM antibody. However we have seen positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

From what I have seen in literature, liver does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2019-06-06

Question

We are currently using anti-CD11b/ITGAM antibody A00144-1 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

The anti-CD11b/ITGAM antibody (A00144-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-23

Question

Will A00144-1 anti-CD11b/ITGAM antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

You can see on the product datasheet, A00144-1 anti-CD11b/ITGAM antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-22

Question

Is PB9140 and A00144-1 suitable for IF application?

Verified customer

Asked: 2019-03-26

Answer

IF test results of the Anti-CD11b/ITGAM Antibody Picoband™ (PB9140) and Anti-CD11b/ITGAM Antibody Picoband™ (A00144-1) were not good. We do not recommend using the antibodies for IF application.

Boster Scientific Support

Answered: 2019-03-26

Question

Is this A00144-1 anti-CD11b/ITGAM antibody reactive to the isotypes of ITGAM?

S. Anderson

Verified customer

Asked: 2018-11-05

Answer

The immunogen of A00144-1 anti-CD11b/ITGAM antibody is E. coli-derived human CD11b recombinant protein (Position: N129-E336). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-11-05

Question

My lab would like to test anti-CD11b/ITGAM antibody A00144-1 on rat bone marrow cell for research purposes, then I may be interested in using anti-CD11b/ITGAM antibody A00144-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

D. Banerjee

Verified customer

Asked: 2018-01-30

Answer

The products we sell, including anti-CD11b/ITGAM antibody A00144-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-01-30

Question

We have tried in the past anti-CD11b/ITGAM antibody for IHC on bone marrow cell a few years ago. I am using rat, and We are going to use the antibody for WB next. Our lab want to know about examining bone marrow cell as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

S. Anderson

Verified customer

Asked: 2017-06-20

Answer

I looked at the website and datasheets of our anti-CD11b/ITGAM antibody and it appears that A00144-1 has been tested on rat in both IHC and WB. Thus A00144-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-06-20

Question

See attached the WB image, lot number and protocol we used for bone marrow cell using anti-CD11b/ITGAM antibody A00144-1. Please let me know if you require anything else.

J. Kulkarni

Verified customer

Asked: 2016-05-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-05-23

Question

Is a blocking peptide available for product anti-CD11b/ITGAM antibody (A00144-1)?

G. Baker

Verified customer

Asked: 2015-07-01

Answer

We do provide the blocking peptide for product anti-CD11b/ITGAM antibody (A00144-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-07-01

Order DetailsPrice
A00144-1

100μg

$370
A00144-1-10ug

10μg sample (liquid)

$99
A00144-1-Biotin

100 μg Biotin conjugated

$570
A00144-1-Cy3

100 μg Cy3 conjugated

$570
A00144-1-Dylight488

100 μg Dylight488 conjugated

$570
A00144-1-Dylight550

100 μg Dylight550 conjugated

$570
A00144-1-Dylight594

100 μg Dylight594 conjugated

$570
A00144-1-FITC

100 μg FITC conjugated

$570
A00144-1-HRP

100 μg HRP conjugated

$570
A00144-1-APC

100 μg APC conjugated

$670
A00144-1-PE

100 μg PE conjugated

$670
A00144-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A00144-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.