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- Table of Contents
Itgax antibody (A00357-3) performs well in ELISA, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IHC analysis of CD11c/Itgax using anti-CD11c/Itgax antibody (A00357-3).
CD11c/Itgax was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD11c/Itgax Antibody (A00357-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
CD11c/Itgax was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD11c/Itgax Antibody (A00357-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-CD11c/Itgax Antibody Picoband®
Itgax antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Itgax antibody was positively detected by WB in:
mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Itgax antibody was positively detected by IHC in:
mouse lung tissue, rat lung tissue, rat lymph node tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Mouse
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Product Information
Product Name
Anti-CD11c/Itgax Antibody Picoband®
View all ITGAX Antibodies
View all ITGAX Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD11c/Itgax recombinant protein (Position: P140-T342).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
127 kDa
Calculated Molecular Weight
129.2 kDa
Background of Itgax
CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a genethat encodes for CD11c. This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-CD11c/Itgax Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00357-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
82%
Moderate Homology
Dog
71%
Moderate Homology
Human
68%
Low homology
Monkey
68%
Low homology
Cow
67%
Low homology
Sheep
67%
Low homology
Rabbit
67%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Itgax antibody
Immunohistochemistry (IHC) protocol for Itgax antibody
Publications
Application:
Species:
High intake of n-6 polyunsaturated fatty acid exacerbates non-alcoholic steatohepatitis by the involvement of multiple metabolic pathways
Published: 2025-06-23
Journal: Frontiers in Nutrition
Application: IF
Impact Factor: 5.10
Species: Rat
PMID: 40626231
1,25-dihydroxyvitamin D3 improves non-alcoholic steatohepatitis phenotype in a diet-induced rat model
Published: 2025-03-21
Journal: Frontiers in Endocrinology
Application: IF
Impact Factor: 4.60
Species: Rat
PMID: 40190400
Dendritic cell-based biomimetic nanoparticles for foot-and-mouth disease induce robust cellular immunity
Published: 2024-09-25
Journal: ANTIVIRAL RESEARCH
Application: WB
Impact Factor: 4.50
Species: Mouse
PMID: 39332536
The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis
Published: 2023-03-07
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IHC
Impact Factor: 8.20
Species: Mouse
PMID: 36889619
Immunotherapy with Dendritic Cells Modified with Tumor-Associated Antigen Gene Demonstrates Enhanced Antitumor Effect Against Lung Cancer
Published: 2017-01-25
Journal: Translational Oncology
Application: IHC
Impact Factor: 3.03
Species: Mouse
PMID: 28129580
Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy
Published: 2014-11-01
Journal: Iranian Journal of Basic Medical Sciences
Application: IHC
Impact Factor: 0.60
Species: Rat
PMID: 25691929
S100+ cells: A new neuro-immune cross-talkers in lymph organs
Published: 2013-01-23
Journal: Scientific Reports
Application: IF
Impact Factor: 2.93
Species: Rat
PMID: 23346371
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Product Promise
We promise Anti-CD11c/Itgax Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00357-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026