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Product Info Summary
| SKU: | M00357-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD11c ITGAX Rabbit Monoclonal Antibody
SKU/Catalog Number
M00357-1
BM4090 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD11c ITGAX Rabbit Monoclonal Antibody catalog # M00357-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11c ITGAX Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00357-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BCE-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD11c
Reactive Species
M00357-1 is reactive to ITGAX in Human
Observed Molecular Weight
128 kDa
Calculated molecular weight
127.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00357-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: THP-1 cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Western blot analysis of CD11c expression in THP-1 cell lysate treated with TPA.
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IHC analysis of CD11c using anti-CD11c antibody (M00357-1).
CD11c was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD11c Antibody (M00357-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of ITGAX using anti-ITGAX antibody (M00357-1).
ITGAX was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ITGAX Antibody (M00357-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-CD11c ITGAX Rabbit Monoclonal Antibody (M00357-1)
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Customer Q&As
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4 Customer Q&As for Anti-CD11c ITGAX Rabbit Monoclonal Antibody
Question
My boss were happy with the WB result of your anti-CD11c Rabbit Monoclonal antibody. However we have observed positive staining in liver membrane using this antibody. Is that expected? Could you tell me where is ITGAX supposed to be expressed?
G. Baker
Verified customer
Asked: 2020-03-09
Answer
Based on literature, liver does express ITGAX. Generally ITGAX expresses in membrane. Regarding which tissues have ITGAX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2020-03-09
Question
We bought anti-CD11c Rabbit Monoclonal antibody for IP on liver a few months ago. I am using human, and We want to use the antibody for IHC next. We want examining liver as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
C. Zhao
Verified customer
Asked: 2020-02-24
Answer
I have checked the website and datasheets of our anti-CD11c Rabbit Monoclonal antibody and it seems that M00357-1 has been validated on human in both IP and IHC. Thus M00357-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-24
Question
We are currently using anti-CD11c Rabbit Monoclonal antibody M00357-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
The anti-CD11c Rabbit Monoclonal antibody (M00357-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-21
Question
We have observed staining in human blood. Do you have any suggestions? Is anti-CD11c Rabbit Monoclonal antibody supposed to stain blood positively?
G. Miller
Verified customer
Asked: 2019-04-11
Answer
According to literature blood does express ITGAX. According to Uniprot.org, ITGAX is expressed in blood, liver, among other tissues. Regarding which tissues have ITGAX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-04-11
