Anti-CD11c ITGAX Rabbit Monoclonal Antibody

CD11c antibody

Boster Bio Anti-CD11c ITGAX Rabbit Monoclonal Antibody catalog # M00357-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Product Info Summary

SKU: M00357-1
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-CD11c ITGAX Rabbit Monoclonal Antibody

View all CD11c Antibodies

SKU/Catalog Number

M00357-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD11c ITGAX Rabbit Monoclonal Antibody catalog # M00357-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD11c ITGAX Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00357-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BCE-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD11c

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00357-1 is reactive to ITGAX in Human

Applications

M00357-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

127.829kDa

Background of CD11c

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ITGAX (Source: Uniprot.org, NCBI)

Gene Name

ITGAX

Full Name

Integrin alpha-X

Weight

127.829kDa

Superfamily

integrin alpha chain family

Alternative Names

95 alpha chain; 95; 95, alpha subunit; CD11 antigen-like family member C; CD11c antigen; CD11c; CD11Cleu M5, alpha subunit; Integrin alpha X; integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAX; Leu M5; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150; leukocyte surface antigen p150; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; p150,95 alpha; SLEB6 ITGAX CD11C, SLEB6 integrin subunit alpha X integrin alpha-X|CD11 antigen-like family member C|complement component 3 receptor 4 subunit|integrin alpha X|integrin, alpha X (antigen CD11C (p150), alpha polypeptide)|integrin, alpha X (complement component 3 receptor 4 subunit)|leu M5, alpha subunit|leukocyte adhesion glycoprotein p150,95 alpha chain|leukocyte adhesion receptor p150,95|leukocyte surface antigen p150,95, alpha subunit|myeloid membrane antigen, alpha subunit|p150 95 integrin alpha chain

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ITGAX, check out the ITGAX Infographic

ITGAX infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITGAX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-CD11c ITGAX Rabbit Monoclonal Antibody

Question

My boss were happy with the WB result of your anti-CD11c Rabbit Monoclonal antibody. However we have observed positive staining in liver membrane using this antibody. Is that expected? Could you tell me where is ITGAX supposed to be expressed?

G. Baker

Verified customer

Asked: 2020-03-09

Answer

Based on literature, liver does express ITGAX. Generally ITGAX expresses in membrane. Regarding which tissues have ITGAX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2020-03-09

Question

We bought anti-CD11c Rabbit Monoclonal antibody for IP on liver a few months ago. I am using human, and We want to use the antibody for IHC next. We want examining liver as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

C. Zhao

Verified customer

Asked: 2020-02-24

Answer

I have checked the website and datasheets of our anti-CD11c Rabbit Monoclonal antibody and it seems that M00357-1 has been validated on human in both IP and IHC. Thus M00357-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-24

Question

We are currently using anti-CD11c Rabbit Monoclonal antibody M00357-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

The anti-CD11c Rabbit Monoclonal antibody (M00357-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-21

Question

We have observed staining in human blood. Do you have any suggestions? Is anti-CD11c Rabbit Monoclonal antibody supposed to stain blood positively?

G. Miller

Verified customer

Asked: 2019-04-11

Answer

According to literature blood does express ITGAX. According to Uniprot.org, ITGAX is expressed in blood, liver, among other tissues. Regarding which tissues have ITGAX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2019-04-11

Order DetailsPrice
M00357-1

100μL

$370
M00357-1-30ul

30μL sample

$99
M00357-1-carrier-free

Carrier Free

$370

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M00357-1
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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