Product Info Summary
SKU: | M02591 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD13 ANPEP Rabbit Monoclonal Antibody
View all Aminopeptidase N/CD13 Antibodies
SKU/Catalog Number
M02591
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD13 ANPEP Rabbit Monoclonal Antibody catalog # M02591. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD13 ANPEP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02591)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GOF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD13
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M02591 is reactive to ANPEP in Human, Mouse, Rat
Applications
M02591 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
410 kDa
Calculated molecular weight
109.54kDa
Background of Aminopeptidase N/CD13
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:100-1:500
ICC/IF 1:100-1:500
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of CD13 expression in THP-1 cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human kidney, using CD13 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ANPEP (Source: Uniprot.org, NCBI)
Gene Name
ANPEP
Full Name
Aminopeptidase N
Weight
109.54kDa
Superfamily
peptidase M1 family
Alternative Names
alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD13 antigen; CD13; CD13APN; EC 3.4.11; EC 3.4.11.2; gp150; LAP1; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; p150; PEPN; PEPNhAPN ANPEP APN, CD13, GP150, LAP1, P150, PEPN alanyl aminopeptidase, membrane aminopeptidase N|AP-M|AP-N|alanyl (membrane) aminopeptidase|aminopeptidase M|hAPN|membrane alanyl aminopeptidase|microsomal aminopeptidase|myeloid plasma membrane glycoprotein CD13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANPEP, check out the ANPEP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANPEP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD13 ANPEP Rabbit Monoclonal Antibody (M02591)
Hello CJ!
M02591 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-CD13 ANPEP Rabbit Monoclonal Antibody
Question
Would anti-CD13 Rabbit Monoclonal antibody M02591 work on zebrafish IP with intestine?
J. Baker
Verified customer
Asked: 2020-04-02
Answer
Our lab technicians have not tested anti-CD13 Rabbit Monoclonal antibody M02591 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CD13 Rabbit Monoclonal antibody M02591 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish intestine in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-02
Question
Would M02591 anti-CD13 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
As indicated on the product datasheet, M02591 anti-CD13 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-02
Question
I see that the anti-CD13 Rabbit Monoclonal antibody M02591 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-16
Question
I am looking for to test anti-CD13 Rabbit Monoclonal antibody M02591 on mouse liver for research purposes, then I may be interested in using anti-CD13 Rabbit Monoclonal antibody M02591 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Moore
Verified customer
Asked: 2019-07-29
Answer
The products we sell, including anti-CD13 Rabbit Monoclonal antibody M02591, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-29
Question
Is there a BSA free version of anti-CD13 Rabbit Monoclonal antibody M02591 available?
B. Jackson
Verified customer
Asked: 2019-05-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD13 Rabbit Monoclonal antibody M02591 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-08
Question
My question regarding product M02591, anti-CD13 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02591 anti-CD13 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-05
Question
I have attached the WB image, lot number and protocol we used for liver using anti-CD13 Rabbit Monoclonal antibody M02591. Please let me know if you require anything else.
T. Banerjee
Verified customer
Asked: 2015-01-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-28