Anti-CD14 Rabbit Monoclonal Antibody

Boster Bio Anti-CD14 Rabbit Monoclonal Antibody catalog # M00137. Tested in IF, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00137
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-CD14 Rabbit Monoclonal Antibody

See all CD14 products

SKU/Catalog Number

M00137

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD14 Rabbit Monoclonal Antibody catalog # M00137. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD14 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00137)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FID-3

Immunogen

A synthesized peptide derived from human CD14

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00137 is reactive to CD14 in Human

Applications

M00137 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For CD14 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08571

Gene ID

929

Gene Name

CD14

Full Name

Monocyte differentiation antigen CD14

Weight

40.076kDa

Alternative Names

CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein CD14 CD14 molecule monocyte differentiation antigen CD14|myeloid cell-specific leucine-rich glycoprotein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD14, check out the CD14 Infographic

CD14 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Anti-CD14 Rabbit Monoclonal Antibody

Question

Is this M00137 anti-CD14 Rabbit Monoclonal antibody reactive to the isotypes of CD14?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The immunogen of M00137 anti-CD14 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD14. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-31

Question

Our lab were well pleased with the WB result of your anti-CD14 Rabbit Monoclonal antibody. However we have seen positive staining in glioblastoma cell membrane using this antibody. Is that expected? Could you tell me where is CD14 supposed to be expressed?

S. Wu

Verified customer

Asked: 2019-12-23

Answer

According to literature, glioblastoma does express CD14. Generally CD14 expresses in cell membrane. Regarding which tissues have CD14 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Glioblastoma, Pubmed ID: 2779588
Liver, Pubmed ID: 19159218, 24275569
Lymphocyte, Pubmed ID: 2453848
Macrophage, Pubmed ID: 2472171
Plasma, Pubmed ID: 16335952
Promyelocytic leukemia, Pubmed ID: 18810425

Boster Scientific Support

Answered: 2019-12-23

Question

Our lab used your anti-CD14 Rabbit Monoclonal antibody for IF on lymphocyte in a previous project. I am using human, and I plan to use the antibody for WB next. I would like examining lymphocyte as well as macrophage in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

I have checked the website and datasheets of our anti-CD14 Rabbit Monoclonal antibody and I see that M00137 has been tested on human in both IF and WB. Thus M00137 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-08

Question

We are currently using anti-CD14 Rabbit Monoclonal antibody M00137 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

The anti-CD14 Rabbit Monoclonal antibody (M00137) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-06

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for promyelocytic leukemia using anti-CD14 Rabbit Monoclonal antibody M00137. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-04

Question

We have observed staining in human liver. Are there any suggestions? Is anti-CD14 Rabbit Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

Based on literature liver does express CD14. Based on Uniprot.org, CD14 is expressed in liver, lymphocyte, macrophage, promyelocytic leukemia, brain, glioblastoma, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have CD14 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Glioblastoma, Pubmed ID: 2779588
Liver, Pubmed ID: 19159218, 24275569
Lymphocyte, Pubmed ID: 2453848
Macrophage, Pubmed ID: 2472171
Plasma, Pubmed ID: 16335952
Promyelocytic leukemia, Pubmed ID: 18810425

Boster Scientific Support

Answered: 2019-06-11

Question

Do you have a BSA free version of anti-CD14 Rabbit Monoclonal antibody M00137 available?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD14 Rabbit Monoclonal antibody M00137 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-28

Question

Will M00137 anti-CD14 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

As indicated on the product datasheet, M00137 anti-CD14 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-14

Question

Will anti-CD14 Rabbit Monoclonal antibody M00137 work for IF with promyelocytic leukemia?

Verified Customer

Verified customer

Asked: 2018-10-12

Answer

According to the expression profile of promyelocytic leukemia, CD14 is highly expressed in promyelocytic leukemia. So, it is likely that anti-CD14 Rabbit Monoclonal antibody M00137 will work for IF with promyelocytic leukemia.

Boster Scientific Support

Answered: 2018-10-12

Question

I was wanting to use your anti-CD14 Rabbit Monoclonal antibody for IF for human promyelocytic leukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human promyelocytic leukemia identification?

Verified Customer

Verified customer

Asked: 2018-04-09

Answer

As indicated on the product datasheet, M00137 anti-CD14 Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human promyelocytic leukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-04-09

Question

Here is the WB image, lot number and protocol we used for promyelocytic leukemia using anti-CD14 Rabbit Monoclonal antibody M00137. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-07-31

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-31

Question

Is a blocking peptide available for product anti-CD14 Rabbit Monoclonal antibody (M00137)?

B. Moore

Verified customer

Asked: 2017-03-21

Answer

We do provide the blocking peptide for product anti-CD14 Rabbit Monoclonal antibody (M00137). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-03-21

Question

you antibody to test anti-CD14 Rabbit Monoclonal antibody M00137 on human promyelocytic leukemia for research purposes, then I may be interested in using anti-CD14 Rabbit Monoclonal antibody M00137 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Miller

Verified customer

Asked: 2016-03-24

Answer

The products we sell, including anti-CD14 Rabbit Monoclonal antibody M00137, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-03-24

Question

I see that the anti-CD14 Rabbit Monoclonal antibody M00137 works with IF, what is the protocol used to produce the result images on the product page?

S. Parker

Verified customer

Asked: 2015-06-04

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-06-04

Question

My question regarding product M00137, anti-CD14 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

C. Lewis

Verified customer

Asked: 2014-02-20

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00137 anti-CD14 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-02-20

Question

Does anti-CD14 Rabbit Monoclonal antibody M00137 work on bovine WB with brain?

E. Bhatt

Verified customer

Asked: 2013-08-21

Answer

Our lab technicians have not tested anti-CD14 Rabbit Monoclonal antibody M00137 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CD14 Rabbit Monoclonal antibody M00137 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-08-21

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M00137
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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