Anti-CD148 PTPRJ Antibody

Product Name

Anti-CD148 PTPRJ Antibody

View all DEP-1/CD148 Antibodies

SKU/Catalog Number

A02319

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CD148 PTPRJ Antibody catalog # A02319. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD148 PTPRJ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02319)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human CD148.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02319 is reactive to PTPRJ in Human

Applications

A02319 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

145.941kDa

Background of DEP-1/CD148

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.

XM Zhou, J. Biol. Chem., Oct 1988; 263: 15584 - 15589.
Felix B. M¨¹ller, Hum. Mol. Genet., Apr 2006; 15: 1133 - 1141.
J Reichelt, J. Cell Sci., Sep 1997; 110: 2175.
BP Korge, PNAS, Feb 1992; 89: 910.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:20000

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of PTPRJ using Anti-CD148 PTPRJ Antibody (A02319).

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver

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Figure 3. Immunohistochemistry validation of PTPRJ using Anti-CD148 PTPRJ Antibody (A02319).

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver

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Figure 4. Immunohistochemistry validation of PTPRJ using Anti-CD148 PTPRJ Antibody (A02319).

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Colon

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Figure 5. Immunohistochemistry validation of PTPRJ using Anti-CD148 PTPRJ Antibody (A02319).

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Colon

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Figure 1. Western blotting validation for Anti-CD148 PTPRJ Antibody A02319

Western Blot (WB) analysis of HeLa

Gene/Protein Information For PTPRJ (Source: Uniprot.org, NCBI)

Alternative Names

CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of PTPRJ CD148, DEP1, HPTPeta, R-PTP-ETA, SCC1 protein tyrosine phosphatase receptor type J receptor-type tyrosine-protein phosphatase eta|CD148 antigen|DEP-1|HPTP eta|R-PTP-J|density-enhanced phosphatase 1|human density enhanced phosphatase-1|protein tyrosine phosphatase, receptor type, J polypeptide|protein-tyrosine phosphatase eta|susceptibility to colon cancer 1, mouse, homolog of

For more info on PTPRJ, check out the PTPRJ Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTPRJ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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