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Product Info Summary
| SKU: | A00020 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-CD152 CTLA4 Antibody
SKU/Catalog Number
A00020
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD152 CTLA4 Antibody catalog # A00020. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD152 CTLA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00020)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Clone Number
1E12E1
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 41-90.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00020 is reactive to CTLA4 in Human
Applications
A00020 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
24.656kDa
Background of CTLA-4
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene (isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For CTLA4 (Source: Uniprot.org, NCBI)
Gene Name
CTLA4
Full Name
Cytotoxic T-lymphocyte protein 4
Weight
24.656kDa
Alternative Names
CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 CTLA4 ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12 cytotoxic T-lymphocyte associated protein 4 cytotoxic T-lymphocyte protein 4|celiac disease 3|cytotoxic T lymphocyte associated antigen 4 short spliced form|cytotoxic T-lymphocyte-associated serine esterase-4|insulin-dependent diabetes mellitus 12|ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTLA4, check out the CTLA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTLA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD152 CTLA4 Antibody (A00020)
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No publications found for A00020
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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14 Customer Q&As for Anti-CD152 CTLA4 Antibody
Question
I see that the anti-CD152 antibody A00020 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-18
Question
Is this A00020 anti-CD152 antibody reactive to the isotypes of CTLA4?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The immunogen of A00020 anti-CD152 antibody is Synthetic peptide from human protein at AA range: 41-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-22
Question
Is a blocking peptide available for product anti-CD152 antibody (A00020)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We do provide the blocking peptide for product anti-CD152 antibody (A00020). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-15
Question
We are interested in to test anti-CD152 antibody A00020 on human lymph node for research purposes, then I may be interested in using anti-CD152 antibody A00020 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
The products we sell, including anti-CD152 antibody A00020, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-12
Question
We are currently using anti-CD152 antibody A00020 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
R. Wu
Verified customer
Asked: 2019-07-03
Answer
The anti-CD152 antibody (A00020) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-03
Question
Does A00020 anti-CD152 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
As indicated on the product datasheet, A00020 anti-CD152 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-15
Question
Can you help my question with product A00020, anti-CD152 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
H. Wu
Verified customer
Asked: 2018-09-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00020 anti-CD152 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-14
Question
Does anti-CD152 antibody A00020 work for IHC with lymph node?
N. Wu
Verified customer
Asked: 2018-09-04
Answer
According to the expression profile of lymph node, CTLA4 is highly expressed in lymph node. So, it is likely that anti-CD152 antibody A00020 will work for IHC with lymph node.
Boster Scientific Support
Answered: 2018-09-04
Question
Do you have a BSA free version of anti-CD152 antibody A00020 available?
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD152 antibody A00020 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-21
Question
I was wanting to use your anti-CD152 antibody for IHC for human lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph node identification?
Verified Customer
Verified customer
Asked: 2018-03-21
Answer
It shows on the product datasheet, A00020 anti-CD152 antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph node in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-21
Question
We were happy with the WB result of your anti-CD152 antibody. However we have been able to see positive staining in lung cell membrane using this antibody. Is that expected? Could you tell me where is CTLA4 supposed to be expressed?
N. Kulkarni
Verified customer
Asked: 2018-01-23
Answer
From what I have seen in literature, lung does express CTLA4. Generally CTLA4 expresses in cell membrane. Regarding which tissues have CTLA4 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Lymph node, Pubmed ID: 10493833
Lymphocyte, Pubmed ID: 3220103
Boster Scientific Support
Answered: 2018-01-23
Question
We have been able to see staining in human lung. What should we do? Is anti-CD152 antibody supposed to stain lung positively?
O. Moore
Verified customer
Asked: 2017-03-28
Answer
From what I have seen in literature lung does express CTLA4. From what I have seen in Uniprot.org, CTLA4 is expressed in lymph node, lung, lymphocyte, among other tissues. Regarding which tissues have CTLA4 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Lymph node, Pubmed ID: 10493833
Lymphocyte, Pubmed ID: 3220103
Boster Scientific Support
Answered: 2017-03-28
Question
I have attached the WB image, lot number and protocol we used for lymph node using anti-CD152 antibody A00020. Please let me know if you require anything else.
B. Gonzalez
Verified customer
Asked: 2014-08-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph node using anti-CD152 antibody A00020. Let me know if you need anything else.
W. Parker
Verified customer
Asked: 2014-07-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-23
