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Product Info Summary
| SKU: | M01788 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD166 Monoclonal Antibody
SKU/Catalog Number
M01788
BM5440 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD166 Monoclonal Antibody catalog # M01788. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD166 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01788)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADGA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD166
Reactive Species
M01788 is reactive to ALCAM in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
65.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01788 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:40
FC 1:60
Positive Control
WB: Daudi cell
Validation Images & Assay Conditions
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Western blot analysis of CD166 expression in Daudi cell lysate.
Specific Publications For Anti-CD166 Monoclonal Antibody (M01788)
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3 Customer Q&As for Anti-CD166 Monoclonal Antibody
Question
Will anti-CD166 Monoclonal antibody M01788 work for IF with lung placenta?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
According to the expression profile of lung placenta, ALCAM is highly expressed in lung placenta. So, it is likely that anti-CD166 Monoclonal antibody M01788 will work for IF with lung placenta.
Boster Scientific Support
Answered: 2020-04-27
Question
Does anti-CD166 Monoclonal antibody M01788 work on feline IP with plasma?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Our lab technicians have not validated anti-CD166 Monoclonal antibody M01788 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CD166 Monoclonal antibody M01788 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline plasma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-27
Question
Would M01788 anti-CD166 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Lewis
Verified customer
Asked: 2014-04-16
Answer
It shows on the product datasheet, M01788 anti-CD166 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-04-16
