Anti-CD234 ACKR1 Antibody

DARC antibody

Boster Bio Anti-CD234 ACKR1 Antibody (Catalog# A30786). Tested in IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Product Info Summary

SKU: A30786
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC

Product Name

Anti-CD234 ACKR1 Antibody

View all DARC Antibodies

SKU/Catalog Number

A30786

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CD234 ACKR1 Antibody (Catalog# A30786). Tested in IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD234 ACKR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30786)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human DARC. Immunogen sequence location: 18264

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30786 is reactive to ACKR1 in Human, Mouse, Rat

Applications

A30786 is guaranteed for ELISA, IF, ICC Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

35.553kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For ACKR1 (Source: Uniprot.org, NCBI)

Gene Name

ACKR1

Full Name

Atypical chemokine receptor 1

Weight

35.553kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

ACKR1; CCBP1; CD234 antigen; CD234; DARC; Dfy; Duffy Antigen; Duffy antigen/chemokine receptor; Duffy blood group antigen; Duffy blood group, chemokine receptor; Fy Antigen; Fy glycoprotein; FYDuffy blood group; Glycoprotein D; GPD; GPDWBCQ1; GpFy; Plasmodium vivax receptor ACKR1 CCBP1, CD234, DARC, DARC/ACKR1, Dfy, FY, GPD, GpFy, WBCQ1 atypical chemokine receptor 1 (Duffy blood group) atypical chemokine receptor 1|Duffy antigen chemokine receptor|Duffy blood group antigen|Duffy blood group system protein|Duffy blood group, chemokine receptor|Fy glycoprotein|glycoprotein D|plasmodium vivax receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACKR1, check out the ACKR1 Infographic

ACKR1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACKR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-CD234 ACKR1 Antibody

Question

Is a blocking peptide available for product anti-CD234 antibody (A30786)?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

We do provide the blocking peptide for product anti-CD234 antibody (A30786). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-12

Question

We need to test anti-CD234 antibody A30786 on human bone marrow for research purposes, then I may be interested in using anti-CD234 antibody A30786 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Wu

Verified customer

Asked: 2019-10-22

Answer

The products we sell, including anti-CD234 antibody A30786, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-22

Question

Would anti-CD234 antibody A30786 work for IF with bone marrow?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

According to the expression profile of bone marrow, ACKR1 is highly expressed in bone marrow. So, it is likely that anti-CD234 antibody A30786 will work for IF with bone marrow.

Boster Scientific Support

Answered: 2019-07-01

Question

We are currently using anti-CD234 antibody A30786 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

The anti-CD234 antibody (A30786) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-03-28

Question

Would A30786 anti-CD234 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Collins

Verified customer

Asked: 2018-08-23

Answer

It shows on the product datasheet, A30786 anti-CD234 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-23

Question

I was wanting to use your anti-CD234 antibody for IF for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?

Verified Customer

Verified customer

Asked: 2017-11-14

Answer

It shows on the product datasheet, A30786 anti-CD234 antibody has been tested for IF on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-14

Question

Would anti-CD234 antibody A30786 work on horse IF with lung?

M. Lewis

Verified customer

Asked: 2016-06-24

Answer

Our lab technicians have not validated anti-CD234 antibody A30786 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CD234 antibody A30786 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse lung in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-06-24

Order DetailsPrice
A30786

100 μL

$335

Free Secondary Antibody

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A30786
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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