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Product Info Summary
| SKU: | PA2057 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD244 Antibody
View all 2B4/CD244/SLAMF4 Antibodies
SKU/Catalog Number
PA2057
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD244 Antibody catalog # PA2057. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD244 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2057)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD244.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2057 is reactive to CD244 in Human
Applications
PA2057 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
41.616kDa
Background of 2B4/CD244/SLAMF4
CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al. (1999) mapped the 2B4 gene to 1q22. Suzuki et al. (2008) identified a functional single-nucleotide polymorphism (SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al. (1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al. (1998) demonstrated that CD48 binds to 2B4. Watzl et al. (2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-CD244 antibody, PA2057, Western blotting
All lanes: Anti CD244 (PA2057) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
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Anti-CD244 antibody, PA2057, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info & Infographic
Gene/Protein Information For CD244 (Source: Uniprot.org, NCBI)
Gene Name
CD244
Full Name
Natural killer cell receptor 2B4
Weight
41.616kDa
Alternative Names
2B4; CD244 antigen; CD244 molecule, natural killer cell receptor 2B4; CD244; h2B4; NAIL; NAILNK cell activation-inducing ligand; natural killer cell receptor 2B4; NKR2B4; NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4; Nmrk; SLAMF4; SLAMF4NK cell activation inducing ligand NAIL CD244 2B4, NAIL, NKR2B4, Nmrk, SLAMF4 CD244 molecule natural killer cell receptor 2B4|CD244 molecule, natural killer cell receptor 2B4|NK cell activation inducing ligand NAIL|NK cell activation-inducing ligand|NK cell type I receptor protein 2B4|SLAM family member 4|h2B4|signaling lymphocytic activation molecule 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD244, check out the CD244 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD244: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD244 Antibody (PA2057)
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No publications found for PA2057
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CD244 Antibody
Question
Does PA2057 anti-CD244 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
As indicated on the product datasheet, PA2057 anti-CD244 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-10
Question
Does anti-CD244 antibody PA2057 work on monkey WB with natural killer cell?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
Our lab technicians have not validated anti-CD244 antibody PA2057 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CD244 antibody PA2057 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey natural killer cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-06
Question
We are currently using anti-CD244 antibody PA2057 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
M. Jones
Verified customer
Asked: 2016-07-06
Answer
The anti-CD244 antibody (PA2057) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-06
Question
Please see the WB image, lot number and protocol we used for lymphoid tissue using anti-CD244 antibody PA2057. Please let me know if you require anything else.
R. Krishna
Verified customer
Asked: 2015-06-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-19
