Product Info Summary
SKU: | M02421-3 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CD247 Monoclonal Antibody
SKU/Catalog Number
M02421-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CD247 Monoclonal Antibody catalog # M02421-3. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD247 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02421-3)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
4B10
Isotype
IgG
Immunogen
Purified recombinant fragment of human CD247 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02421-3 is reactive to CD247 in Human
Applications
M02421-3 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
18.696kDa
Background of CD247
Anti-Procalcitonin antibody detects human Procalcitonin. Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, a N terminal residue, calcitonin and katacalcin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or shock. This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 4. Flow Cytometry validation of CD247 using Anti-CD247 Monoclonal Antibody (M02421-3).
Flow cytometric (FCM) analysis of Jurkat cells using CD247 Monoclonal Antibody (green) and negative control (purple).Click image to see more details
Figure 3. Immunofluorescent staining data of CD247 using Anti-CD247 Monoclonal Antibody (M02421-3).
Immunofluorescence (IF) analysis of K562 cells using CD247 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.Click image to see more details
Figure 2. Immunohistochemistry validation of CD247 using Anti-CD247 Monoclonal Antibody (M02421-3).
Immunohistochemistry (IHC) analysis of paraffin-embedded human Thymus tissues with AEC staining using CD247 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-CD247 Monoclonal Antibody M02421-3
Western Blot (WB) analysis using CD247 Monoclonal Antibody against CD247-hIgGFc transfected HEK293 cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CD247 (Source: Uniprot.org, NCBI)
Gene Name
CD247
Full Name
T-cell surface glycoprotein CD3 zeta chain
Weight
18.696kDa
Superfamily
CD3Z/FCER1G family
Alternative Names
CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain CD247 CD3-ZETA, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCRZ CD247 molecule T-cell surface glycoprotein CD3 zeta chain|CD247 antigen, zeta subunit|CD3Z antigen, zeta polypeptide (TiT3 complex)|CD3zeta chain|T-cell antigen receptor complex, zeta subunit of CD3|T-cell receptor T3 zeta chain|TCR zeta chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD247, check out the CD247 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD247: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD247 Monoclonal Antibody (M02421-3)
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No publications found for M02421-3
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Customer Reviews
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