Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody

Product Name

Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody

View all CD27/TNFRSF7 Antibodies

SKU/Catalog Number

M01148

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody (Catalog # M01148). Tested in ELISA, Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01148)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: LPFS2/1611

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human CD27 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01148 is reactive to CD27 in Human

Applications

M01148 is guaranteed for ELISA, Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

29.137kDa

Background of CD27/TNFRSF7

Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD27 Mouse Monoclonal Antibody (LPFS2/1611)

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD27 Mouse Monoclonal Antibody (LPFS2/1611).

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Formalin-fixed, paraffin-embedded human Spleen stained with Anti-CD27 Mouse Monoclonal Antibody (LPFS2/1611).

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Formalin-fixed, paraffin-embedded human Colon stained with Anti-CD27 Mouse Monoclonal Antibody (LPFS2/1611).

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Flow Cytometric analysis of Ramos cells using Anti-CD27 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence staining of Ramos cells using Anti-CD27 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Gene/Protein Information For CD27 (Source: Uniprot.org, NCBI)

Alternative Names

CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7 CD27 S152, S152. LPFS2, T14, TNFRSF7, Tp55 CD27 molecule CD27 antigen|CD27L receptor|T cell activation antigen S152|T-cell activation antigen CD27|tumor necrosis factor receptor superfamily, member 7

For more info on CD27, check out the CD27 Infographic

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In this infographic, you will see the following information for CD27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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