Anti-CD276 Antibody

Product Name

Anti-CD276 Antibody

View all B7-H3/CD276 Antibodies

SKU/Catalog Number

M02220-3

Size

50 µl

Description

Boster Bio Anti-CD276 Antibody (Catalog # M02220-3). Tested in WB, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-CD276 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02220-3)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

2009CT652.35.9

Isotype

IgG1,κ

Immunogen

This CD276 antibody is generated from a mouse immunized with a recombinant protein from the human region of human CD276.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02220-3 is reactive to CD276 in Human

Applications

M02220-3 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57.235kDa

Background of B7-H3/CD276

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:4000
IHC: 1:400
IHC-P: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-CD276 Antibody at 1:4000 dilution
Lane 1: 293T-17 whole cell lysate
Lane 2: A-431 whole cell lysate
Lane 3: U-20S whole cell lysate
Lane 4: LNCap whole cell lysate
Lane 5: Hela whole cell lysate
Lane 6: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1. CD276 Antibody was diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

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Immunohistochemical analysis of CD276 Antibody in 293T(untransfected) cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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Immunohistochemical analysis of CD276 Antibody in MCF-7 cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Gene/Protein Information For CD276 (Source: Uniprot.org, NCBI)

Alternative Names

B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule CD276 4Ig-B7-H3, B7-H3, B7H3, B7RP-2 CD276 molecule CD276 antigen|B7 homolog 3|costimulatory molecule

For more info on CD276, check out the CD276 Infographic

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In this infographic, you will see the following information for CD276: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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