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Product Info Summary
| SKU: | M02220-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335
View all B7-H3/CD276 Antibodies
SKU/Catalog Number
M02220-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335 (Catalog # M02220-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02220-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM335
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD276 (B7-H3).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the cytoplasmic domain of human CD276 (B7-H3).
Reactive Species
M02220-1 is reactive to CD276 in Human
Applications
M02220-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
57.235kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:5000-1:10,000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of LNCaP cell lysate using anti-CD276 (B7-H3) rabbit monoclonal antibody (Clone RM335) at a 1:10,000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-CD276 (B7-H3) rabbit monoclonal antibody (Clone RM335) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD276 (Source: Uniprot.org, NCBI)
Gene Name
CD276
Full Name
CD276 antigen
Weight
57.235kDa
Superfamily
immunoglobulin superfamily
Alternative Names
B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule CD276 4Ig-B7-H3, B7-H3, B7H3, B7RP-2 CD276 molecule CD276 antigen|B7 homolog 3|costimulatory molecule
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD276, check out the CD276 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD276: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335 (M02220-1)
Hello CJ!
No publications found for M02220-1
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335
Question
We are currently using anti-CD276 (B7-H3) Rabbit Monoclonal antibody, Clone#RM335 M02220-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The anti-CD276 (B7-H3) Rabbit Monoclonal antibody, Clone#RM335 (M02220-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
