Anti-CD3 CD3E Antibody (Monoclonal, 35C62)

Product Name

Anti-CD3 CD3E Antibody (Monoclonal, 35C62)

View all CD3E Antibodies

SKU/Catalog Number

M02675-11

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD3 CD3E Antibody (Monoclonal, 35C62) catalog # M02675-11. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD3 CD3E Antibody (Monoclonal, 35C62) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02675-11)

Host

Mouse

Contents

Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.

Clonality

Monoclonal

Clone Number

35C62

Isotype

Mouse IgG1

Immunogen

Human thymocytes followed by Sezary T cells.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

M02675-11 is reactive to CD3E in Human

Background of CD3E

The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02675-11 is guaranteed for Flow Cytometry, IHC, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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See full target information CD3E

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