Anti-CD3 CD3E Antibody (Monoclonal, CA-3)

Boster Bio Anti-CD3 CD3E Antibody (Monoclonal, CA-3) catalog # MA1015. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human. Cited in 11 publication(s).

Product Info Summary

SKU: MA1015
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IHC, ICC

Product Name

Anti-CD3 CD3E Antibody (Monoclonal, CA-3)

See all CD3E products

SKU/Catalog Number

MA1015

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD3 CD3E Antibody (Monoclonal, CA-3) catalog # MA1015. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD3 CD3E Antibody (Monoclonal, CA-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1015)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

CA-3

Isotype

Mouse IgG1

Immunogen

Human thymocytes followed by Sezary T cells.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1015 is reactive to CD3E in Human

Applications

MA1015 is guaranteed for Flow Cytometry, IHC, ICC Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD3E (Source: Uniprot.Org, NCBI)

Uniprot ID

P07766

Gene ID

916

Gene Name

CD3E

Full Name

T-cell surface glycoprotein CD3 epsilon chain

Weight

23.147kDa

Alternative Names

CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e antigen, epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD3E, check out the CD3E Infographic

CD3E infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD3E: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1015 has been cited in 11 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis

Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients

Chen B, Liu L, Xu H, Yang Y, Zhang L, Zhang F. Exp Ther Med. 2015 Mar;9(3):1063-1067. Epub 2015 Jan 26. Effectiveness Of Immune Therapy Combined With Chemotherapy On The Immune Function And Recurrence Rate Of Cervical Cancer.

Yu X, Li L, Li Q, Zang X, Liu Z. Biol Trace Elem Res. 2011 Nov;143(2):1064-76. Doi: 10.1007/S12011-010-8941-5. Epub 2011 Jan 12. Trail And Dr5 Promote Thyroid Follicular Cell Apoptosis In Iodine Excess-Induced Experimental Autoimmune Thyroiditis I...

Zhang R, Zhou Sj, Li Cj, Wang Xn, Tang Yz, Chen R, Lv L, Zhao Q, Xing Ql, Yu Dm, Yu P. Lipids Health Dis. 2013 Mar 26;12:42. Doi: 10.1186/1476-511X-12-42. C-Reactive Protein/Oxidised Low-Density Lipoprotein/??2-Glycoprotein I Complex Promotes Athe...

Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

Xie Zj, Jia Lm, He Yc, Gao Jt. World J Gastroenterol. 2006 Mar 21;12(11):1757-60. Morphological Observation Of Tumor Infiltrating Immunocytes In Human Rectal Cancer.

Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.

Li Y, Geng S, Du X, Chen S, Yang L, Wu X, Li B, Schmidt Ca, Przybylski Gk. Hematology. 2011 Jan;16(1):43-9. Doi: 10.1179/102453311X12902908411634. Restricted Trbv Repertoire In Cd4+ And Cd8+ T-Cell Subsets From Cml Patients.

Liu Yz, Cao Yg, Ye Jq, Wang Wg, Song Kj, Wang Xl, Wang Ch, Li Rt, Deng Xm. Fitoterapia. 2010 Mar;81(2):108-14. Doi: 10.1016/J.Fitote.2009.08.005. Epub 2009 Aug 15. Immunomodulatory Effects Of Proanthocyanidin A-1 Derived In Vitro From Rhododendron...

Jia H, Wang Y, Tong Xj, Liu Gb, Li Q, Zhang Lx, Sun Xh. Artif Cells Blood Substit Immobil Biotechnol. 2011 Dec;39(6):366-75. Doi: 10.3109/10731199.2011.618133. Epub 2011 Oct 14. Biocompatibility Of Acellular Nerves Of Different Mammalian Species F...

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17 Customer Q&As for Anti-CD3 CD3E Antibody (Monoclonal, CA-3)

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD3 antibody (Monoclonal, CA-3) MA1015. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-20

Question

We are currently using anti-CD3 antibody (Monoclonal, CA-3) MA1015 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

The anti-CD3 antibody (Monoclonal, CA-3) (MA1015) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-25

Question

I was wanting to use your anti-CD3 antibody (Monoclonal, CA-3) for Flow Cytometry for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

You can see on the product datasheet, MA1015 anti-CD3 antibody (Monoclonal, CA-3) has been validated for Flow Cytometry, IHC, ICC on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-12

Question

I am looking for to test anti-CD3 antibody (Monoclonal, CA-3) MA1015 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD3 antibody (Monoclonal, CA-3) MA1015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

The products we sell, including anti-CD3 antibody (Monoclonal, CA-3) MA1015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-14

Question

Does anti-CD3 antibody (Monoclonal, CA-3) MA1015 work on zebrafish Flow Cytometry with blood?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

Our lab technicians have not tested anti-CD3 antibody (Monoclonal, CA-3) MA1015 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CD3 antibody (Monoclonal, CA-3) MA1015 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish blood in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-09

Question

Is a blocking peptide available for product anti-CD3 antibody (Monoclonal, CA-3) (MA1015)?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

We do provide the blocking peptide for product anti-CD3 antibody (Monoclonal, CA-3) (MA1015). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-06

Question

I see that the anti-CD3 antibody (Monoclonal, CA-3) MA1015 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-19

Question

Do you have a BSA free version of anti-CD3 antibody (Monoclonal, CA-3) MA1015 available?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD3 antibody (Monoclonal, CA-3) MA1015 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-29

Question

Would anti-CD3 antibody (Monoclonal, CA-3) MA1015 work for Flow Cytometry with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

According to the expression profile of leukemic t-cell, CD3E is highly expressed in leukemic t-cell. So, it is likely that anti-CD3 antibody (Monoclonal, CA-3) MA1015 will work for Flow Cytometry with leukemic t-cell.

Boster Scientific Support

Answered: 2019-06-13

Question

Does MA1015 anti-CD3 antibody (Monoclonal, CA-3) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-24

Answer

You can see on the product datasheet, MA1015 anti-CD3 antibody (Monoclonal, CA-3) as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-24

Question

Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-CD3 antibody (Monoclonal, CA-3) MA1015. Please let me know if you require anything else.

L. Johnson

Verified customer

Asked: 2018-07-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-17

Question

We ordered your anti-CD3 antibody (Monoclonal, CA-3) for ICC on blood a few years ago. I am using human, and We intend to use the antibody for Flow Cytometry next. I was wanting to use examining blood as well as leukemic t-cell in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?

A. Parker

Verified customer

Asked: 2018-05-22

Answer

I looked at the website and datasheets of our anti-CD3 antibody (Monoclonal, CA-3) and it seems that MA1015 has been validated on human in both ICC and Flow Cytometry. Thus MA1015 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-05-22

Question

My question regarding product MA1015, anti-CD3 antibody (Monoclonal, CA-3). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-21

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1015 anti-CD3 antibody (Monoclonal, CA-3), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-21

Question

We were content with the WB result of your anti-CD3 antibody (Monoclonal, CA-3). However we have been able to see positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is CD3E supposed to be expressed?

C. Roberts

Verified customer

Asked: 2016-09-22

Answer

From what I have seen in literature, blood does express CD3E. Generally CD3E expresses in cell membrane. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-09-22

Question

We are interested in using your anti-CD3 antibody (Monoclonal, CA-3) for t cell receptor signaling pathway studies. Has this antibody been tested with western blotting on human pbmc cells? We would like to see some validation images before ordering.

A. Zhao

Verified customer

Asked: 2016-09-15

Answer

We appreciate your inquiry. This MA1015 anti-CD3 antibody (Monoclonal, CA-3) is tested on human pbmc cells. It is guaranteed to work for Flow Cytometry, IHC, ICC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-09-15

Question

Is this MA1015 anti-CD3 antibody (Monoclonal, CA-3) reactive to the isotypes of CD3E?

P. Jha

Verified customer

Asked: 2015-04-28

Answer

The immunogen of MA1015 anti-CD3 antibody (Monoclonal, CA-3) is Human thymocytes followed by Sezary T cells. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-04-28

Question

We have observed staining in human leukocyte. What should we do? Is anti-CD3 antibody (Monoclonal, CA-3) supposed to stain leukocyte positively?

R. Kulkarni

Verified customer

Asked: 2015-03-11

Answer

Based on literature leukocyte does express CD3E. Based on Uniprot.org, CD3E is expressed in leukocyte, blood, thymus, leukemic t-cell, among other tissues. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2015-03-11

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MA1015
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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