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Product Info Summary
|Application:||Flow Cytometry, IHC, ICC|
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Anti-CD3 CD3E Antibody (Monoclonal, CA-3)
Boster Bio Anti-CD3 CD3E Antibody (Monoclonal, CA-3) catalog # MA1015. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD3 CD3E Antibody (Monoclonal, CA-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1015)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Human thymocytes followed by Sezary T cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
MA1015 is reactive to CD3E in Human
MA1015 is guaranteed for Flow Cytometry, IHC, ICC Boster Guarantee
Background of CD3E
The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD3 antibody (MA1015).
Overlay histogram showing human PBMC cells stained with MA1015 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD3 Antibody (MA1015,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse gG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Gene/Protein Information For CD3E (Source: Uniprot.org, NCBI)
T-cell surface glycoprotein CD3 epsilon chain
CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e antigen, epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on CD3E, check out the CD3E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD3E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
MA1015 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Chen B, Liu L, Xu H, Yang Y, Zhang L, Zhang F. Exp Ther Med. 2015 Mar;9(3):1063-1067. Epub 2015 Jan 26. Effectiveness Of Immune Therapy Combined With Chemotherapy On The Immune Function And Recurrence Rate Of Cervical Cancer.
Yu X, Li L, Li Q, Zang X, Liu Z. Biol Trace Elem Res. 2011 Nov;143(2):1064-76. Doi: 10.1007/S12011-010-8941-5. Epub 2011 Jan 12. Trail And Dr5 Promote Thyroid Follicular Cell Apoptosis In Iodine Excess-Induced Experimental Autoimmune Thyroiditis I...
Zhang R, Zhou Sj, Li Cj, Wang Xn, Tang Yz, Chen R, Lv L, Zhao Q, Xing Ql, Yu Dm, Yu P. Lipids Health Dis. 2013 Mar 26;12:42. Doi: 10.1186/1476-511X-12-42. C-Reactive Protein/Oxidised Low-Density Lipoprotein/??2-Glycoprotein I Complex Promotes Athe...
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...
Xie Zj, Jia Lm, He Yc, Gao Jt. World J Gastroenterol. 2006 Mar 21;12(11):1757-60. Morphological Observation Of Tumor Infiltrating Immunocytes In Human Rectal Cancer.
Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
Li Y, Geng S, Du X, Chen S, Yang L, Wu X, Li B, Schmidt Ca, Przybylski Gk. Hematology. 2011 Jan;16(1):43-9. Doi: 10.1179/102453311X12902908411634. Restricted Trbv Repertoire In Cd4+ And Cd8+ T-Cell Subsets From Cml Patients.
Liu Yz, Cao Yg, Ye Jq, Wang Wg, Song Kj, Wang Xl, Wang Ch, Li Rt, Deng Xm. Fitoterapia. 2010 Mar;81(2):108-14. Doi: 10.1016/J.Fitote.2009.08.005. Epub 2009 Aug 15. Immunomodulatory Effects Of Proanthocyanidin A-1 Derived In Vitro From Rhododendron...
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