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Pack Size:100μg/vial
Validated Species:Human
Clone Number:CA-3
Application:Flow Cytometry, IHC, ICC
Data & Images


Product Name Anti-CD3 Antibody (Monoclonal, CA-3)
SKU/Catalog Number MA1015
Description Mouse IgG monoclonal antibody for CD3, CD3e molecule, epsilon (CD3-TCR complex) (CD3E) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.
Cite This Product Anti-CD3 Antibody (Monoclonal, CA-3) CA-3 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1015)
Replacement Item This antibody may replace the following items: sc-5765|sc-59614|sc-5767|sc-20768|sc-376262|sc-20768|sc-376262|sc-5765|sc-5767|sc-59614 from Santa Cruz Biotechnology.
Host Mouse
Validated Species Human
Application Flow Cytometry, IHC, ICC

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen Human thymocytes followed by Sezary T cells.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Monoclonal
Clone Number CA-3
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cd3e
Protein Name T-cell surface glycoprotein CD3 epsilon chain
Molecular Weight 21312 MW
Protein Function CD3E: The CD3 complex mediates signal transduction.
Sequence Similarities Contains 1 Ig-like (immunoglobulin-like) domain.
Subcellular Localization CD3E: Membrane; Single-pass type I membraneprotein.
Cd3e: Cell membrane
Uniprot ID D4A5M2
Alternative Names CD3 antigen, epsilon polypeptide (Predicted) ;Protein Cd3e ;Cd3e ;Cd3e_predicted ;rCG_58471 ;
Research Areas |immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|hematopoietic stem cells|human lineage negative| immunology|regulatory t cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for T-cell surface glycoprotein CD3 epsilon chain

The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3(formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.

Anti-CD3 Antibody (Monoclonal, CA-3) Images

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Anti-CD3 Antibody (Monoclonal, CA-3)
Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD3 antibody (MA1015).
Overlay histogram showing human PBMC cells stained with MA1015 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD3 Antibody (MA1015,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse gG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Customer Q&As

Q: What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applications
A: The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd3 antibody, t cell receptor antibody, t3 antibody