SKU MA1015
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number CA-3
Host Mouse
Ig Isotype Mouse IgG1
Applications Flow Cytometry, IHC, ICC

Overview

Product Name Anti-CD3 Antibody (Monoclonal, CA-3)
SKU/Catalog Number MA1015
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for CD3, CD3e molecule, epsilon (CD3-TCR complex) (CD3E) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.
Cite This Product Anti-CD3 Antibody (Monoclonal, CA-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1015)
Host Mouse
Immunogen Human thymocytes followed by Sezary T cells.
Reactivity Human

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Images And Assay Conditions

/m/a/ma1015 cd3_ primary antibodies fc testing 1.jpg

Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD3 antibody (MA1015).
Overlay histogram showing human PBMC cells stained with MA1015 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD3 Antibody (MA1015,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse gG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id D4A5M2
Gene Name CD3E
Protein Name CD3e molecule, epsilon(CD3-TCR complex)
Alternative Names CD3 antigen, epsilon polypeptide (Predicted) ;Protein Cd3e ;Cd3e ;Cd3e_predicted ;rCG_58471 ;
Subcellular Localization CD3E: Membrane; Single-pass type I membraneprotein.|Cd3e: Cell membrane
Molecular Weight 21312 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function CD3E: The CD3 complex mediates signal transduction.
Background The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3(formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.

Order Product (MA1015)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships in 5-7 business days.

Troubleshooting

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Publications

The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis
Effectiveness of immune therapy combined with chemotherapy on the immune function and recurrence rate of cervical cancer
S100+cells: A new neuro-immune cross-talkers in lymph organs
Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients
Morphological observation of tumor infiltrating immunocytes in human rectal cancer
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Chen B, Liu L, Xu H, Yang Y, Zhang L, Zhang F. Exp Ther Med. 2015 Mar;9(3):1063-1067. Epub 2015 Jan 26. Effectiveness Of Immune Therapy Combined With Chemotherapy On The Immune Function And Recurrence Rate Of Cervical Cancer.
Yu X, Li L, Li Q, Zang X, Liu Z. Biol Trace Elem Res. 2011 Nov;143(2):1064-76. Doi: 10.1007/S12011-010-8941-5. Epub 2011 Jan 12. Trail And Dr5 Promote Thyroid Follicular Cell Apoptosis In Iodine Excess-Induced Experimental Autoimmune Thyroiditis I...
Zhang R, Zhou Sj, Li Cj, Wang Xn, Tang Yz, Chen R, Lv L, Zhao Q, Xing Ql, Yu Dm, Yu P. Lipids Health Dis. 2013 Mar 26;12:42. Doi: 10.1186/1476-511X-12-42. C-Reactive Protein/Oxidised Low-Density Lipoprotein/??2-Glycoprotein I Complex Promotes Ather...
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...
Xie Zj, Jia Lm, He Yc, Gao Jt. World J Gastroenterol. 2006 Mar 21;12(11):1757-60. Morphological Observation Of Tumor Infiltrating Immunocytes In Human Rectal Cancer.
Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
Li Y, Geng S, Du X, Chen S, Yang L, Wu X, Li B, Schmidt Ca, Przybylski Gk. Hematology. 2011 Jan;16(1):43-9. Doi: 10.1179/102453311X12902908411634. Restricted Trbv Repertoire In Cd4+ And Cd8+ T-Cell Subsets From Cml Patients.
Liu Yz, Cao Yg, Ye Jq, Wang Wg, Song Kj, Wang Xl, Wang Ch, Li Rt, Deng Xm. Fitoterapia. 2010 Mar;81(2):108-14. Doi: 10.1016/J.Fitote.2009.08.005. Epub 2009 Aug 15. Immunomodulatory Effects Of Proanthocyanidin A-1 Derived In Vitro From Rhododendron...
Jia H, Wang Y, Tong Xj, Liu Gb, Li Q, Zhang Lx, Sun Xh. Artif Cells Blood Substit Immobil Biotechnol. 2011 Dec;39(6):366-75. Doi: 10.3109/10731199.2011.618133. Epub 2011 Oct 14. Biocompatibility Of Acellular Nerves Of Different Mammalian Species F...

Customer Q&As

Q: What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applications
A: The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd3 antibody, t cell receptor antibody, t3 antibody