|Application:||Flow Cytometry, IHC, ICC|
Data & Images
|Product Name||Anti-CD3 Antibody (Monoclonal, CA-3)|
|Description||Mouse IgG monoclonal antibody for CD3, CD3e molecule, epsilon (CD3-TCR complex) (CD3E) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CD3 Antibody (Monoclonal, CA-3) CA-3 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1015)|
|Replacement Item||This antibody may replace the following items: sc-5765|sc-59614|sc-5767|sc-20768|sc-376262|sc-20768|sc-376262|sc-5765|sc-5767|sc-59614 from Santa Cruz Biotechnology.|
|Application||Flow Cytometry, IHC, ICC
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Human thymocytes followed by Sezary T cells.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||T-cell surface glycoprotein CD3 epsilon chain|
|Molecular Weight||21312 MW|
|Protein Function||CD3E: The CD3 complex mediates signal transduction.|
|Sequence Similarities||Contains 1 Ig-like (immunoglobulin-like) domain.|
|Subcellular Localization||CD3E: Membrane; Single-pass type I membraneprotein.
Cd3e: Cell membrane
|Alternative Names||CD3 antigen, epsilon polypeptide (Predicted) ;Protein Cd3e ;Cd3e ;Cd3e_predicted ;rCG_58471 ;|
|Research Areas|||immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|hematopoietic stem cells|human lineage negative| immunology|regulatory t cells||
Background for T-cell surface glycoprotein CD3 epsilon chain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD3 Antibody (Monoclonal, CA-3) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 1μg/ml, Human|
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD3 Antibody (Monoclonal, CA-3) Images
Click the images to enlarge.
Overlay histogram showing human PBMC cells stained with MA1015 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD3 Antibody (MA1015,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.