|Application:||Flow Cytometry, IHC, ICC|
Data & Images
|Product Name||Anti-CD3 Antibody (Monoclonal, CA-3)|
|Description||Mouse IgG monoclonal antibody for CD3, CD3e molecule, epsilon (CD3-TCR complex) (CD3E) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CD3 Antibody (Monoclonal, CA-3) CA-3 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1015)|
|Replacement Item||This antibody may replace the following items: sc-5765|sc-59614|sc-5767|sc-20768|sc-376262|sc-20768|sc-376262|sc-5765|sc-5767|sc-59614 from Santa Cruz Biotechnology.|
|Application||Flow Cytometry, IHC, ICC
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Human thymocytes followed by Sezary T cells.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||T-cell surface glycoprotein CD3 epsilon chain|
|Molecular Weight||21312 MW|
|Protein Function||CD3E: The CD3 complex mediates signal transduction.|
|Sequence Similarities||Contains 1 Ig-like (immunoglobulin-like) domain.|
|Subcellular Localization||CD3E: Membrane; Single-pass type I membraneprotein.
Cd3e: Cell membrane
|Alternative Names||CD3 antigen, epsilon polypeptide (Predicted) ;Protein Cd3e ;Cd3e ;Cd3e_predicted ;rCG_58471 ;|
|Research Areas|||immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|hematopoietic stem cells|human lineage negative| immunology|regulatory t cells||
Background for T-cell surface glycoprotein CD3 epsilon chain
Anti-CD3 Antibody (Monoclonal, CA-3) Images
Click the images to enlarge.
Overlay histogram showing human PBMC cells stained with MA1015 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD3 Antibody (MA1015,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse gG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.