Anti-CD3 Antibody (Monoclonal, CA-3)
|Ig Isotype||Mouse IgG1|
|Applications||Flow Cytometry, IHC, ICC|
|Product Name||Anti-CD3 Antibody (Monoclonal, CA-3)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for CD3, CD3e molecule, epsilon (CD3-TCR complex) (CD3E) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CD3 Antibody (Monoclonal, CA-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1015)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Human thymocytes followed by Sezary T cells.|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
Images And Assay Conditions
Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD3 antibody (MA1015).
Overlay histogram showing human PBMC cells stained with MA1015 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD3 Antibody (MA1015,1Î¼g/1x106 cells) for 30 min at 20Â°C. DyLightÂ®488 conjugated goat anti-mouse IgG (BA1126, 5-10Î¼g/1x106 cells) was used as secondary antibody for 30 minutes at 20Â°C. Isotype control antibody (Green line) was mouse gG (1Î¼g/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info (Source: Uniprot.org)
|Protein Name||CD3e molecule, epsilon(CD3-TCR complex)|
|Alternative Names||CD3 antigen, epsilon polypeptide (Predicted) ;Protein Cd3e ;Cd3e ;Cd3e_predicted ;rCG_58471 ;|
|Subcellular Localization||CD3E: Membrane; Single-pass type I membraneprotein.|Cd3e: Cell membrane|
|Molecular Weight||21312 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||CD3E: The CD3 complex mediates signal transduction.|
*You can search these to find other products in these research areas.
|Background||The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3(formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.|
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Q: What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applicationsA: The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact firstname.lastname@example.org
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to email@example.com.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to cd3 antibody, t cell receptor antibody, t3 antibody