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Product Info Summary
| SKU: | A05953-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CD300A Antibody Picoband®
SKU/Catalog Number
A05953-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD300A Antibody Picoband® catalog # A10247-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD300A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05953-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human CD300A recombinant protein (Position: L156-T299). Human CD300A shares 54.7% and 55.7% amino acid (aa) sequence identity with mouse and rat CD300A, respectively.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05953-1 is reactive to CD300A in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
33.2 kDa
Background of CD300A
CD300A (Cluster of Differentiation 300A) is a human gene. This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05953-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HL-60 whole cell, human Raji whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse Raw2647 whole cell, mouse J774A1 whole cell
FCM: U20S cell
Validation Images & Assay Conditions
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Western blot analysis of CD300A using anti-CD300A antibody (A05953-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse Raw264.7 whole cell lysates,
Lane 6: mouse J774A.1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD300A antigen affinity purified polyclonal antibody (Catalog # A05953-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD300A at approximately 68 kDa. The expected band size for CD300A is at 33 kDa.
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Flow Cytometry analysis of U20S cells using anti-CD300A antibody (A05953-1).
Overlay histogram showing U20S cells stained with A05953-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD300A Antibody (A05953-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-CD300A Antibody Picoband® (A05953-1)
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