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Product Info Summary
| SKU: | A09624 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD315 PTGFRN Antibody
SKU/Catalog Number
A09624
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD315 PTGFRN Antibody catalog # A09624. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD315 PTGFRN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09624)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human PTGFRN. AA range:601-650
Reactive Species
A09624 is reactive to PTGFRN in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
98.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09624 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:100-300
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using CD315 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200
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Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.
Specific Publications For Anti-CD315 PTGFRN Antibody (A09624)
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5 Customer Q&As for Anti-CD315 PTGFRN Antibody
Question
We are currently using anti-CD315 antibody A09624 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The anti-CD315 antibody (A09624) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD315 antibody A09624. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-30
Question
Is a blocking peptide available for product anti-CD315 antibody (A09624)?
J. Edwards
Verified customer
Asked: 2018-01-04
Answer
We do provide the blocking peptide for product anti-CD315 antibody (A09624). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-04
Question
Will anti-CD315 antibody A09624 work on bovine IHC with embryo?
S. Jones
Verified customer
Asked: 2017-11-22
Answer
Our lab technicians have not validated anti-CD315 antibody A09624 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CD315 antibody A09624 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine embryo in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-22
Question
Is there a BSA free version of anti-CD315 antibody A09624 available?
N. Parker
Verified customer
Asked: 2016-08-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD315 antibody A09624 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-08-01
