Anti-CD328 SIGLEC7 Antibody

Siglec-7/CD328 antibody

Boster Bio Anti-CD328 SIGLEC7 Antibody catalog # A06553. Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A06553
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CD328 SIGLEC7 Antibody

View all Siglec-7/CD328 Antibodies

SKU/Catalog Number

A06553

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CD328 SIGLEC7 Antibody catalog # A06553. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD328 SIGLEC7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06553)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2B4

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human CD328.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06553 is reactive to SIGLEC7 in Human

Applications

A06553 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

51.143kDa

Background of Siglec-7/CD328

Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC-P, 1:100-300

Validation Images & Assay Conditions

Gene/Protein Information For SIGLEC7 (Source: Uniprot.org, NCBI)

Gene Name

SIGLEC7

Full Name

Sialic acid-binding Ig-like lectin 7

Weight

51.143kDa

Superfamily

immunoglobulin superfamily

Alternative Names

Adhesion inhibitory receptor molecule 1; adhesion inhibitory receptor molecule 1, siglec-7; AIRM1; AIRM-1; AIRM1QA79 membrane protein; CD328 antigen; CD328; CDw328; D-siglec; p75; p75/AIRM1; QA79; sialic acid binding Ig-like lectin 7; sialic acid binding immunoglobulin-like lectin 7; sialic acid-binding Ig-like lectin 7; Siglec7; Siglec-7 SIGLEC7 AIRM-1, AIRM1, CD328, CDw328, D-siglec, QA79, SIGLEC-7, SIGLEC19P, SIGLECP2, p75, p75/AIRM1 sialic acid binding Ig like lectin 7 sialic acid-binding Ig-like lectin 7|QA79 membrane protein|adhesion inhibitory receptor molecule 1, siglec-7|sialic acid binding immunoglobulin-like lectin 7

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SIGLEC7, check out the SIGLEC7 Infographic

SIGLEC7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SIGLEC7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-CD328 SIGLEC7 Antibody

Question

Does A06553 anti-CD328 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Taylor

Verified customer

Asked: 2019-08-13

Answer

It shows on the product datasheet, A06553 anti-CD328 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-13

Question

We are currently using anti-CD328 antibody A06553 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

The anti-CD328 antibody (A06553) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-05

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD328 antibody A06553. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-22

Question

Can you help my question with product A06553, anti-CD328 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-10-11

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06553 anti-CD328 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-10-11

Question

Is there a BSA free version of anti-CD328 antibody A06553 available?

Verified Customer

Verified customer

Asked: 2018-07-17

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD328 antibody A06553 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-07-17

Question

Would anti-CD328 antibody A06553 work for WB with liver?

Verified Customer

Verified customer

Asked: 2018-05-18

Answer

According to the expression profile of liver, SIGLEC7 is highly expressed in liver. So, it is likely that anti-CD328 antibody A06553 will work for WB with liver.

Boster Scientific Support

Answered: 2018-05-18

Question

Is a blocking peptide available for product anti-CD328 antibody (A06553)?

V. Lewis

Verified customer

Asked: 2013-09-13

Answer

We do provide the blocking peptide for product anti-CD328 antibody (A06553). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-09-13

Order DetailsPrice
A06553

100uL

$399

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A06553
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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