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Product Info Summary
| SKU: | A06553 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD328 SIGLEC7 Antibody
SKU/Catalog Number
A06553
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD328 SIGLEC7 Antibody catalog # A06553. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD328 SIGLEC7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06553)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human SIGLEC7. AA range:51-100
Reactive Species
A06553 is reactive to SIGLEC7 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
51.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06553 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Recommend Dilution
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of K562 cells using CD328 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from K562 cells, using SIGLEC7 Antibody.
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Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:100
Specific Publications For Anti-CD328 SIGLEC7 Antibody (A06553)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CD328 SIGLEC7 Antibody
Question
Does A06553 anti-CD328 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Taylor
Verified customer
Asked: 2019-08-13
Answer
It shows on the product datasheet, A06553 anti-CD328 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-13
Question
We are currently using anti-CD328 antibody A06553 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The anti-CD328 antibody (A06553) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD328 antibody A06553. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-22
Question
Can you help my question with product A06553, anti-CD328 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06553 anti-CD328 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-11
Question
Is there a BSA free version of anti-CD328 antibody A06553 available?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD328 antibody A06553 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-17
Question
Would anti-CD328 antibody A06553 work for WB with liver?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
According to the expression profile of liver, SIGLEC7 is highly expressed in liver. So, it is likely that anti-CD328 antibody A06553 will work for WB with liver.
Boster Scientific Support
Answered: 2018-05-18
Question
Is a blocking peptide available for product anti-CD328 antibody (A06553)?
V. Lewis
Verified customer
Asked: 2013-09-13
Answer
We do provide the blocking peptide for product anti-CD328 antibody (A06553). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-09-13
