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- Table of Contents
Cd33 antibody (RP1105) performs well in IHC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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CD33 was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody (Catalog # RP1105) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Designing a Western blot for CD33? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CD33 Western Blot GuideBundle Save
Anti-CD33 Antibody Picoband®
Cd33 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | IHC, WB |
Tested Applications
Cd33 antibody was positively detected by WB in:
mosue lung extract (mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse
Western blot
0.1-0.5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-CD33 Antibody Picoband®
View all CD33 Antibodies
View all CD33 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.01 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD33 recombinant protein (Position: D18-E240). Mouse CD33 shares 61.6% amino acid (aa) sequence identity with human CD33.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated Molecular Weight
44.8 kDa
Background of Cd33
CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.
Cite This Product
Anti-CD33 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1105)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
87%
Moderate Homology
Dog
61%
Low homology
Human
60%
Low homology
Monkey
58%
Low homology
Cow
54%
Low homology
Zebrafish
24%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cd33 antibody
Publications
Application:
Species:
Inhibition of lung cancer in diethylnitrosamine-induced mice by Rhizoma paridis saponins
Published: 2016-12-19
Journal: MOLECULAR CARCINOGENESIS
Application: WB
Impact Factor: 4.72
Species: Mouse
PMID: 27991692
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Product Promise
We promise Anti-CD33 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
RP1105
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 25, 2026