Anti-CD40 / TNFRSF5 Monoclonal Antibody

Product Name

Anti-CD40 / TNFRSF5 Monoclonal Antibody

View all CD40/TNFRSF5 Antibodies

SKU/Catalog Number

M00068

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD40 / TNFRSF5 Monoclonal Antibody (Catalog # M00068). Tested in IF, Flow Cytometry, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD40 / TNFRSF5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00068)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: C40/1605

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human CD40 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00068 is reactive to CD40 in Human

Applications

M00068 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

30.619kDa

Background of CD40/TNFRSF5

CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunofluorescence (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD40 Mouse Monoclonal Antibody (C40/1605).

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Formalin-fixed, paraffin-embedded human Hodgkin’s Lymphoma stained with Anti-CD40 Mouse Monoclonal Antibody (C40/1605).

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Formalin-fixed, paraffin-embedded human Spleen stained with Anti-CD40 Mouse Monoclonal Antibody (C40/1605).

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SDS-PAGE analysis purified Anti-CD40 Mouse Monoclonal Antibody (C40/1605). Confirmation of integrity and purity of antibody.

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Flow Cytometric analysis of U2OS cells using Anti-CD40 Mouse Monoclonal Antibody (C40/1605) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Gene/Protein Information For CD40 (Source: Uniprot.org, NCBI)

Alternative Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface antigen CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor

For more info on CD40, check out the CD40 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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